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3.6.5.4: signal-recognition-particle GTPase

This is an abbreviated version!
For detailed information about signal-recognition-particle GTPase, go to the full flat file.

Word Map on EC 3.6.5.4

Reaction

GTP
+
H2O
=
GDP
+
phosphate

Synonyms

54-kDa GTPase, chloroplast signal recognition particle, chloroplast signal recognition particle protein, chloroplast signal recognition particle receptor, chloroplast SRP, cpFtsY, cpSRP, cpSRP43, cpSRP54, CtSR, EC 3.6.1.49, Ffh, FlhF, FtsH, FtsY, FtsY GTPase, GTPase, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, PAB0955, ribosomal GTPase, signal recognition particle, signal recognition particle 54 kDa protein, signal recognition particle receptor, signal recognition particle receptor beta subunit, signal recognition particle receptor subunit alpha, signal recognition particle receptor subunit beta, signal recognition particle-like GTPase, signal-recognition-particle GTPase, SR, SR GTPase, SRalpha, SRbeta, SRbeta GTPase, SRP, SRP GTPase, SRP GTPase Ffh, SRP receptor, SRP receptor GTPase, SRP14, SRP19, SRP21, SRP54, SRP54 GTPase, Srp72p, SRP:SR GTPase, SRP:SR guanine triphosphatase, SRPRA, SRPRB

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.4 signal-recognition-particle GTPase

Purification

Purification on EC 3.6.5.4 - signal-recognition-particle GTPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ffh NG is purified
-
Ffh NG is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column
-
FtsY NGd20 is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column
-
glutathione Sepharose column chromatography
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography
HiPrep heparin Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration
Ni-Sepharose column chromatography
-
proteins are purified using histidine-binding magnetic agarose beads
-
recombinant C-terminally His6-tagged wild-type and mutant mature cpSRP54 (residues 76-564) from Escherichia coli strain BL21 Star
recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration
Q9DBG7; P47758
recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3)
Thermochaetoides thermophila
recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration
P08240; Q9Y5M8
recombinant His6-tagged signal recognition particle protein cpSRP54 from Escherichia coli strain Rosetta by nickel affinity chromatography, cation exchange chromatography, and anion exchange chromatography
recombinant wild-type and mutant cpSRP54 from Escherichia coli strain Rosetta BL21 by two steps of cation exchange chromatography
-
the FtsY NGd20 protein is purified over a HiTrap Blue column, desalted, then passed over a HiTrap SP and a HiTrap Q Sepharose column
-