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3.6.5.4: signal-recognition-particle GTPase

This is an abbreviated version!
For detailed information about signal-recognition-particle GTPase, go to the full flat file.

Word Map on EC 3.6.5.4

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

54-kDa GTPase, chloroplast signal recognition particle, chloroplast signal recognition particle protein, chloroplast signal recognition particle receptor, chloroplast SRP, cpFtsY, cpSRP, cpSRP43, cpSRP54, CtSR, EC 3.6.1.49, Ffh, FlhF, FtsH, FtsY, FtsY GTPase, GTPase, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, PAB0955, ribosomal GTPase, signal recognition particle, signal recognition particle 54 kDa protein, signal recognition particle receptor, signal recognition particle receptor beta subunit, signal recognition particle receptor subunit alpha, signal recognition particle receptor subunit beta, signal recognition particle-like GTPase, signal-recognition-particle GTPase, SR, SR GTPase, SRalpha, SRbeta, SRbeta GTPase, SRP, SRP GTPase, SRP GTPase Ffh, SRP receptor, SRP receptor GTPase, SRP14, SRP19, SRP21, SRP54, SRP54 GTPase, Srp72p, SRP:SR GTPase, SRP:SR guanine triphosphatase, SRPRA, SRPRB

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.4 signal-recognition-particle GTPase

Cloned

Cloned on EC 3.6.5.4 - signal-recognition-particle GTPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a construct of Thermus aquaticus FtsY in which the first 20 amino acids are deleted is subsequently expressed
-
cpFtsY is cloned and expressed in Escherichia coli
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expressed in Escherichia coli
-
expressed in Escherichia coli B834(DE3)-Rosetta2 cells
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)-Rosetta2 cells
expressed in Escherichia coli BL21-AI cells
expressed in Escherichia coli BL21-CodonPlus(DE3) cells and in COS-1 cells
-
expression in Escherichia coli
expression of wild-type and mutant cpSRP54 in Escherichia coli strain Rosetta BL21
-
Ffh NG is expressed
-
gene CPSRP54, recombinant overexpression of His6-tagged signal recognition particle protein cpSRP54 in Escherichia coli strain Rosetta, coexpression with signal recognition particle activator protein cpFtsY forming the cpSRP54-cpFtsY complex
gene CTHT_0050420, sequence comparisons
Thermochaetoides thermophila
genes ftsY and ffh, sequence comparisons
into the vectors pTD37 and pET22b
-
N and G domains of Ffh, expression in Escherichia coli BL21(DE3)-Rosetta
-
recombinant expression of C-terminally His6-tagged wild-type and mutant mature cpSRP54 (residues 76-564) in Escherichia coli strain BL21 Star
recombinant expression of His-tagged chimeric enzyme constructs in Escherichia coli strain BL21 (DE3)
Q9DBG7; P47758
recombinant expression of His-tagged chimeric enzyme constructs in Escherichia coli strain BL21(DE3)
recombinant large-scale production of all human SRP components and the reconstitution of homogeneous signal recognition particle (SRP) and signal recognition particle receptor (SR) complexes
P08240; Q9Y5M8
the Escherichia coli strain TOP10 is used for general cloning stategies and the strain SCS110 as an intermediary host in Bacillus cereus transformation experiments, the vectors pRN5101 and pDG148 are used
-
the NG domain of Ffh is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells
-
the NG domain of FtsY is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells
-
the NG domain of Thermus aquaticus Ffh is cloned and subsequently expressed
-
the vector pJGS3 expressing the protein FtsY NGd20, in which the N-terminal residues 2-20 of FtsY are deleted, is constructed by PCR-based site-directed mutagenesis of pTB88 for expression in Escherichia coli Rosetta-2DE3 pLysS cells
-