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3.6.5.1: heterotrimeric G-protein GTPase

This is an abbreviated version!
For detailed information about heterotrimeric G-protein GTPase, go to the full flat file.

Word Map on EC 3.6.5.1

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

cone transducin, EC 3.6.1.46, EF-2, EF-G, elongation factor 2, G alpha i2, G alpha q, G alpha11, G alphaQ, G betagamma, G12, G13, Galpha protein, Galpha-subunit, Galpha16, Galphai1, Galphai3, Galphao, Galphaq, GI, GmGalpha protein, GPA1, Gq, Gs alpha, GTP phosphohydrolase, GTPase, guanosine 5'-triphosphatase, guanosine triphosphatase, heterotrimeric G protein, heterotrimeric G protein alpha subunit, heterotrimeric G protein family, heterotrimeric G protein Gsalpha, heterotrimeric G-protein, heterotrimeric G-protein GTPase, heterotrimeric GTPase, phosphatase, guanosine tri-, photoreceptor-specific G protein, Rab1, Rab1 GTPase, Rab1a, ribosomal GTPase, SsEF-2, transducin, transducin GTPase

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.1 heterotrimeric G-protein GTPase

Cloned

Cloned on EC 3.6.5.1 - heterotrimeric G-protein GTPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of heterotrimeric G proteins, DNA and amino acid sequence determination and analysis
-
cloning of the complete heterotrimeric G-protein repertoire in the Capsicum annuum genome which consists of one Galpha, one Gbeta and three Ggamma genes, phylogenetic analysis, DNA and amino acid sequence determination and analysis, real time quantitative PCR tissue-specific enzyme expression analysis, transgenic expression of Gbeta, Ggamma1-3 genes, from pSAT5-DEST-cEYFP-C1 destination vectors containing cEYFP at the N-terminal end via Agrobacterium tumefaciens strain GV3101 transformation, in Nicotiana tabaccum leaves, co-expression of RGS protein
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expressed in HEK-293T cells
-
expression in Escherichia coli
-
Expression of the N-terminally His6-tagged mutant Galphai/q chimeric gene, in which the native N-terminus of Galphaq is replaced with that of Galphai1, the gene also posseses a TEV cleavage site, amino acids 1-28 of rat Galphai1, a linker of Arg and Ser, and the 37-359 amino acid region of mouse Galphaq, expression of recombinant wild-type Galphaq and Galphaq mutant Q209E in Mus musculus Galphaq/11-deficient embryonic fibroblasts, Swiss3T3 cells, expression of alpha subunit cDNAs of heterotrimeric GTPases, Gs, Gi-2, G13 and G11 in human 293T cells
-
Galpha subunit is subcloned into pcDNA3.1+
-
genes gna 1-3 encoding the three subunits of the enzyme, transfection of Neurospora crassa with mutant enzymes
Q05425; Q05424; Q9HFW7
Gtalpha and GtalphaR238E for expression in Escherichia coli strains DH5alpha and BL21(DE3)
-
into pcDNA3.1 vector for transfection of HEK-293 cells
-
into the pT7-7 vector for expession in Escherichia coli JM109DE3 cells
-
into vector for expression in Escherichia coli
-
overexpression of heterotrimeric G-proteins Galphai1 and Galphai3 in Spodoptera frugiperda Sf 9 cell membranes using the baculovirus transfection method
-
pBRH Gtalpha transgenic construct containing the mouse Gtalpha genomic sequence flanked by the mouse opsin promoter fragment and the polyadenylation signal, EE-tagged
-
Rab1a is cloned from a mouse cardiac cDNA library, adenoviruses expressing FLAG-Rab1a are generated for infection of rat ventricular myocytes
-
recombinant expression of His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 in Escherichia coli strain M15
-
the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified
-