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3.6.4.B10: chaperonin ATPase (protein-folding, protecting from aggregation, protein stabilizing)

This is an abbreviated version!
For detailed information about chaperonin ATPase (protein-folding, protecting from aggregation, protein stabilizing), go to the full flat file.

Word Map on EC 3.6.4.B10

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

alpha/beta-thermosome, ApCpnB, archaeal chaperonin, CCT, CCT ATPase, CCT chaperonin, CCT complex, CCT/TRiC, CCT/TRiC chaperonin, CCT/TRiC complex, CCT2, chaperonin B, chaperonin containing t-complex polypeptide-1, chaperonin containing TCP-1, chaperonin containing TCP1, chaperonin TCP-1 ring complex, chaperonin TRiC, chaperonin TRiC/CCT, chaperonin-containing t-complex polypeptide 1, cPKA, CpkB, CtCCT, cytosolic chaperonin TRiC, eukaryotic chaperone complex, eukaryotic chaperonin, eukaryotic chaperonin TRiC, eukaryotic group II chaperonin, GaTRiC, group II chaperonin, group II chaperonin CCT/TRiC, group II CPN, HsTRiC, Mm-cpn, MmCpn, More, PfCPN, PfTRiC, PhCPN, Plasmodium TRiC, protein P45, T-complex polypeptide-1 ring complex, T-complex protein 1, T-complex protein 1 ring complex, T-complex protein-1 ring complex, TCP-1, TCP-1 ring complex, TCP1, TCP1 ring complex, thermosome, TKS1-CPN, TriC, TRiC chaperonin, TRiC chaperonin complex, TRiC/CCT, type II chaperonin

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.B10 chaperonin ATPase (protein-folding, protecting from aggregation, protein stabilizing)

Purification

Purification on EC 3.6.4.B10 - chaperonin ATPase (protein-folding, protecting from aggregation, protein stabilizing)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
each of the recombinant alpha- and beta- subunits is purified to 92% and 94% by using anion exchange chromatography
native enzyme by anion exchange chromatography and gel filtration
A0A1S6LQX4; A0A1S6LQU3; A0A1S6LQU0; A0A1S6LQU6; A0A1S6LQU1; A0A1S6LQU9; A0A1S6LQW6; A0A1S6LQW7
native enzyme by calmodulin affinity chromatography and gel filtration
P12612; P39076; P39077; P39078; P40413; P39079; P42943; P47079
native enzyme from bovine testes by anion exchange chromatography and gel filtration
Q32L40; Q3ZBH0; Q3T0K2; F1N0E5; F1MWD3; Q3MHL7; Q2NKZ1; Q3ZCI9
native enzyme from HeLa cells by anion exchange chromatography and gel filtration
P17987; P78371; P49368; P50991; P48643; P40227; Q99832; P50990
native enzyme is purified from HeLa cells by a process including anion exchange chromatography and gel filtration
P17987; P78371; P49368; P50991; P48643; P40227; Q99832; P50990
recombinant alpha-subunit from Escherichia coli strains Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) by heat shock treatment and anion exchange chromatography
recombinant beta-subunit from Escherichia coli strains Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) by heat shock treatment and anion exchange chromatography
recombinant chaperonin Mm-cpn from Escherichia coli strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, and gel filtation, followed by ultrafiltration
recombinant enzyme
-
recombinant enzyme CPN from Escherichia coli strain BLR(DE3)/pET23a by heat treatment for 15 min at 90°C, anion exchange chromatography, ultrafiltration, and gel filtration
-
recombinant His-tagged wild-type and mutant enzyme oligomer rings from Escherichia coli
recombinant Strep-tagged wild-type and mutant CCT complexes from Escherichia coli strain BL21 Star(DE3)pRARE by affinity and anion exchange chromatography, and gel filtration
Thermochaetoides thermophila
-
recombinant subunit alpha and subunit beta
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) RIL by heat treatment at 85°C for 30 min, anion exchange chromatography, and gel filtration
the recombinant enzyme is purified to 91% by heat-shock treatment and anion-exchange chromatography