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3.6.4.6: vesicle-fusing ATPase

This is an abbreviated version!
For detailed information about vesicle-fusing ATPase, go to the full flat file.

Word Map on EC 3.6.4.6

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

AAA ATPase Vps4, AAA-ATPase, ATP phosphohydrolase, ATP6V0D1, ATP6V0D2, ATPase, ATPase N-ethylmaleimide sensitive factor, C06A1.1, C41C4.8, CDC-48.1, CDC-48.2, Cdc48, Cdc48p, Csc1p, Did6p, dNSF, dNSF-1, dNSF1, End13p, Grd13p, Helar-NSF, Hez-NSF, HsPEX1, HsPex1p, HsPex6p, membrane deformation AAA ATPase, N-ethyl maleimide sensitive factor, N-ethylmaleimide sensitive factor, N-ethylmaleimide sensitive fusion protein, N-ethylmaleimide sensitive-factor, N-ethylmaleimide-sensitive factor, N-ethylmaleimide-sensitive fusion protein, N-ethylmeleimide-sensitive factor, NEM-sensitive fusion protein, NSF, NSF ATPase, NSF protein, NSF-1, NSF2, nsfA, NSFII, p97, p97/VCP/Cdc48p, p97/VCP/Cdc48p homologue, p97ATPase, peptide p97, Pex1, Pex1p, pex22, Pex6p, PpPex1p, PpPex6p, rat Pex6p, Sec18, SEC18 gene product, SEC18p, SKD1, SKD1/VPS4B, SKD2, SKD2 protein, Sso0909, SsoVps4, suppresor of potassium transport growth defect, Suppressor of K+ Transport Growth Defect1, triple-A ATPase, V-ATPase, vacular protein sorting 4 B, vacuolar protein sorting 4, vacuolar protein sorting 4 protein, vasolin-containing protein, VCP, vesicle-fusing ATPase, Vesicular-fusion protein NSF, Vpl4p, Vps 4B, Vps4, Vps4 ATPase, VPS4 gene product, Vps4p, Vps4p AAA ATPase, Vpt10p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.6 vesicle-fusing ATPase

Engineering

Engineering on EC 3.6.4.6 - vesicle-fusing ATPase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E232Q
-
ATPase-deficient mutant
E329Q
-
in contrast to wild-type NFS E329Q mutant is enriched on various structures throughout the cell and is only minimally released by saponin permeabilization
E219K
R154C
naturally occurring mutation, constitutes variations in surface-exposed locations, but does not alter the ATPase activity
R154C/E219K
naturally occurring mutation, constitutes variations in surface-exposed locations. The E219K mutation leads to an almost complete loss of activity, which is partially recuperated in the R154C/E219K double-mutant indicating p97 inter-domain communication
E209K
site-directed mutagenesis
E233Q
-
inactive
E329Q
-
no ATPase activity
K266A
-
no nucleotide binding
K549A
-
decrease in NSF vesicular transport activity
R155C
naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity, and a twofold decreased kd for human protein UBXD9
R155H
naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity, no change in the kd value for human protein UBXD9
R93C
naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity and a twofold increased kd for human protein UBXD9
D100A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
D142A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
E153A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
G282E
-
mutant protein has no ATPase activity
E235Q
-
no ATPase activity, mutation leads to perturbation of various membrane transports via endosomes
E305Q
-
mutant assembles into oligomer like wild-type, similar ATPase activity as wild-type
E305Q/E578Q
-
no ATPase activity
E578Q
-
mutant assembles into oligomer like wild-type, severely impaired ATPase activity
S569A
-
mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1
K251A
K524A
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
R359A
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
R635A
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
S569A
-
mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1
A505L
-
lethal mutation
E233Q
E243A
site-directed mutagenesis, a pore loop mutant, displays essentially unchanged oligomerization, introducing E243A point mutation increases the affinity of peptide C binding by 3fold, shows reduced ATPase activity compared to the wild-type
E243A/E247A
site-directed mutagenesis, pore loop mutant, displays essentially unchanged oligomerization, the double mutant binds peptides 4fold (peptide C) or 10fold (peptide B) more tightly than wild-type Vps4p, shows reduced ATPase activity compared to the wild-type
E247A
site-directed mutagenesis, a pore loop mutant, displays essentially unchanged oligomerization, introducing E247A point mutation increases the affinity of peptide C binding by 4fold, shows reduced ATPase activity compared to the wild-type
E329Q
-
hydrolysis mutant
E350Q
G89D
-
higher intrinsic ATPase activity than wild-type, no stimulation by Sec17
K179A
-
no ATP binding, enhanced binding to Vps20p and Vta1p in vitro
K266A
-
ATP-binding mutant
Q216A
monomeric mutant form
R241A
site-directed mutagenesis, a pore loop 2 mutant, does not bind peptides with appreciable affinity
R251A
site-directed mutagenesis, a pore loop 2 adjacent mutant, does not bind peptides with appreciable affinity
T240A
site-directed mutagenesis, a pore loop mutant, displays essentially unchanged oligomerization
T240F
site-directed mutagenesis, a pore loop 2 mutant
T240K
site-directed mutagenesis, a pore loop 2 mutant
T240V
site-directed mutagenesis, a pore loop 2 mutant
W206A
site-directed mutagenesis, a pore loop 1 mutant, does not bind peptides with appreciable affinity
additional information