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3.6.4.10: non-chaperonin molecular chaperone ATPase

This is an abbreviated version!
For detailed information about non-chaperonin molecular chaperone ATPase, go to the full flat file.

Word Map on EC 3.6.4.10

Reaction

ATP
+
H2O
=
ADP
+
phosphate

Synonyms

70 kD heat shock protein, 90 kDa heat shock protein, 90-kDa heat shock protein, AAA+ ATPase, AAA+ ATPase ClpB, AAA+ chaperone, AAA+ chaperone ClpB, ATP-dependent foldase, ATPase, ATPase chaperone, ATPase ClpB, bATPase, BiP, bona fide chaperone, chaperone ClpB, chaperone hsp90, chaperone Hsp90alpha, chaperone Hsp90beta, ClpB, ClpB ATPase, ClpB chaperone, ClpB disaggregase, ClpB1, ClpB2, ClpB2/HSP101, ClpC, ClpC chaperone, ClpX, ClpX heat-shock protein, disaggregase, DnaK, DnaK ATPase, DnaK chaperone, endoplasmic reticulum chaperone, ER lumenal hsc70 BiP, eukaryotic Hsc70 ATPase, GroEl, GRP75, Grp78, hATPase, heat shock cognate 70, heat shock cognate protein, heat shock protein, heat shock protein 70, heat shock protein 90, heat shock protein 90-alpha, heat shock protein GroEl, heat shock protein-70, heat shock protein-90, heat-shock cognate protein 70, heat-shock protein 70, heat-shock protein 90, Hsc66, Hsc70, Hsc82, Hsp100, HSP101, Hsp104, Hsp14.0, Hsp19.7, Hsp40, Hsp60, Hsp70, Hsp70 chaperone, Hsp70 chaperone Ssa1, Hsp70-1, Hsp70-2, Hsp70-3, HSP70.1, HSP70.2, Hsp82, Hsp82p, Hsp90, Hsp90 molecular chaperone, Hsp90a, Hsp90alpha, HSPA1L, HspA8, HspA9, HtpG, human alphabeta crystallin, human Hsp70 molecular chaperone, human stress70c, inducible heat shock protein 70, Kar2, maize stress70er, MecB protein, mitochondrial chaperone, mitochondrial heat shock protein 40, mitochondrial heat shock protein 70, mitochondrial heat-shock protein 70, mitochondrial hsp70, molecular chaperone, molecular chaperone BiP, molecular chaperone GroEl, molecular chaperone Hsc70 ATPase, mortalin, mortalin/mtHsp70, mouse alphabeta crystallin, mtHsp40, mtHSP70, p97-valosin-containing protein, p97-VCP, PBP74, PfClpB1, PfHsp70, PoHsp70, Pt-Hsp70, Ssq1, stress70 protein, SyClpC, tomato stress70c, vacuolar H+-ATPase, vacuolar membrane ATPase, yHsp90

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.10 non-chaperonin molecular chaperone ATPase

Purification

Purification on EC 3.6.4.10 - non-chaperonin molecular chaperone ATPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
both wild-type and recombinant proteins
Co-NTA agarose column chromatography and Superdex S200 gel filtration
DEAE-Sephacel, ATP-agarose
-
HisPur cobalt resin column chromatography and Superdex S200 gel filtration
-
HisTrap column chromatography and Superdex S200 gel filtration
-
isolation of the 44-kDa ATPase domain after limited proteolysis
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex S200 gel filtration
nucleotide-binding domain (residue 1-381), 44 kDa
-
Q-Sepharose column chromatography and Sephacryl S-200 gel filtration
recombinant ClpB
-
recombinant ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446, Q-Sepharose, Superdex-200
-
recombinant DnaK
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration
recombinant His-tagged SUMO fusion enzyme mtHSP70 from Escherichia coli by nickel affinity chromatography, His-tag and SUMO cleavage and again nickel affinity exchange chromatography for tag removal, followed by gel filtration
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 pLysS by nickel affinity chromatograhy
recombinant Hsp104
-
recombinant Hsp90
-
recombinant Hsp90, metal affinity, gel filtration, ion-exchange chromatography
-
recombinant murine BiP, expressed in Escherichia coli
-
recombinant p97-VCP
-
recombinant PfHsp70, nickel-chelating Sepharose affinity chromatography
-
recombinant protein
-
recombinant wild-type and mutant enzymes by gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type Hsc7, expressed in Escherichia coli
-
recombinant yHsp90
-