Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.6.4.10: non-chaperonin molecular chaperone ATPase

This is an abbreviated version!
For detailed information about non-chaperonin molecular chaperone ATPase, go to the full flat file.

Word Map on EC 3.6.4.10

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

70 kD heat shock protein, 90 kDa heat shock protein, 90-kDa heat shock protein, AAA+ ATPase, AAA+ ATPase ClpB, AAA+ chaperone, AAA+ chaperone ClpB, ATP-dependent foldase, ATPase, ATPase chaperone, ATPase ClpB, bATPase, BiP, bona fide chaperone, chaperone ClpB, chaperone hsp90, chaperone Hsp90alpha, chaperone Hsp90beta, ClpB, ClpB ATPase, ClpB chaperone, ClpB disaggregase, ClpB1, ClpB2, ClpB2/HSP101, ClpC, ClpC chaperone, ClpX, ClpX heat-shock protein, disaggregase, DnaK, DnaK ATPase, DnaK chaperone, endoplasmic reticulum chaperone, ER lumenal hsc70 BiP, eukaryotic Hsc70 ATPase, GroEl, GRP75, Grp78, hATPase, heat shock cognate 70, heat shock cognate protein, heat shock protein, heat shock protein 70, heat shock protein 90, heat shock protein 90-alpha, heat shock protein GroEl, heat shock protein-70, heat shock protein-90, heat-shock cognate protein 70, heat-shock protein 70, heat-shock protein 90, Hsc66, Hsc70, Hsc82, Hsp100, HSP101, Hsp104, Hsp14.0, Hsp19.7, Hsp40, Hsp60, Hsp70, Hsp70 chaperone, Hsp70 chaperone Ssa1, Hsp70-1, Hsp70-2, Hsp70-3, HSP70.1, HSP70.2, Hsp82, Hsp82p, Hsp90, Hsp90 molecular chaperone, Hsp90a, Hsp90alpha, HSPA1L, HspA8, HspA9, HtpG, human alphabeta crystallin, human Hsp70 molecular chaperone, human stress70c, inducible heat shock protein 70, Kar2, maize stress70er, MecB protein, mitochondrial chaperone, mitochondrial heat shock protein 40, mitochondrial heat shock protein 70, mitochondrial heat-shock protein 70, mitochondrial hsp70, molecular chaperone, molecular chaperone BiP, molecular chaperone GroEl, molecular chaperone Hsc70 ATPase, mortalin, mortalin/mtHsp70, mouse alphabeta crystallin, mtHsp40, mtHSP70, p97-valosin-containing protein, p97-VCP, PBP74, PfClpB1, PfHsp70, PoHsp70, Pt-Hsp70, Ssq1, stress70 protein, SyClpC, tomato stress70c, vacuolar H+-ATPase, vacuolar membrane ATPase, yHsp90

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.10 non-chaperonin molecular chaperone ATPase

Cloned

Cloned on EC 3.6.4.10 - non-chaperonin molecular chaperone ATPase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from spleen cDNA, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 pLysS, co-expression with His-tagged EhAha1c
DnaK (residue 1-638, residue 1-605, residue 1-388)
-
Escherichia coli-based overexpression system
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain M15
-
expressed in Escherichia coli strain Rosetta(DE3)
-
expression in Escherichia coli
expression of ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446 in Escherichia coli
-
expression of GFP-Hsp70 fusion protein in HeLa cells
-
expression of His-tagged PfHsp70 in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene coding for ClpX sequenced
-
gene PnHSP70, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant transient expression of GFP-tagged enzyme expression in Arabidopsis thaliana mesophyll protoplast mitochondria, real-time PCR enzyme expression analysis
-
heat-shock cognate protein 70
Hsc 70, Hsc70 nucleotide binding domain (residue 1-381)
-
Hsp104 is expressed as a transgene in a human leukemic T-cell line (PEER). Hsp104 inhibited heat-shock-induced loss of viability in PEER cells, and Hsp104 cooperates with endogenous human Hsp70 and Hsc70 molecular chaperones and their J-domain-containing cochaperones Hdj1 and Hdj2 to produce a functional hybrid bichaperone network capable of refolding aggregated luciferase. Hsp104 shuttles across the nuclear envelope and enhances the chaperoning capacity of both the cytoplasm and nucleoplasm of intact cells
-
Hsp90beta, Hsp90beta-delta, Hsp90 C-terminal domain (residue 546-724), Hsp90beta-MC (residue 546-724)
-
nucleotide-binding domain (residue 1-381), 44 kDa
-
p9/25PCR1 consists of a 2.4-kb cDNA encoding maize pre-stress70er cloned and expressed in Escherichia coli
-
pLeHsc70-2 consists of a 2.1-kb cDNA encoding tomato stress70c cloned into pUC19
-
pT7-mBiP containing the cDNA sequence encoding mature mouse BiP cloned and expressed in Escherichia coli BL21
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), production of folded and soluble recombinant mortalin when co-expressed with the human Hsp70-escort protein 1, but Hsp70 is still likely prone to self-association
recombinant expression of His-tagged enzyme mtHSP70 as N-terminal SUMO fusion protein, lacking the gene sequence coding for N-terminal mitochondrial targeting peptide, in Escherichia coli
-
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant wild-type Hsc70, cloned and expressed in Escherichia coli
-
strains carrying plasmid pRLM163 designed for overexpression of a cloned dnaK gene
-
two different hsp90 cDNAs are isolated and characterized. Although nearly identical, an additional eight nucleotide sequence is present at the end of the 3'UTP of one of the two cDNAs
-