3.6.1.59: 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase
This is an abbreviated version!
For detailed information about 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase, go to the full flat file.
Word Map on EC 3.6.1.59
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3.6.1.59
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heterodimer
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m7gdp
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disability
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nucleoside
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hint
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exosome
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diauxie
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craniofacial
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trehalase
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exonucleolytic
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intellectual
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medicine
- 3.6.1.59
- heterodimer
- m7gdp
- disability
- nucleoside
-
hint
-
exosome
-
diauxie
-
craniofacial
- trehalase
-
exonucleolytic
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intellectual
- medicine
Reaction
Synonyms
Dcps, Dcs1, EC 3.6.1.30, m7GpppN m7GMP phosphohydrolase, m7GpppX pyrophosphatase, scavenger mRNA decapping enzyme, yDcps, YLR270W
ECTree
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Substrates Products
Substrates Products on EC 3.6.1.59 - 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase
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REACTION DIAGRAM
5'-[GpppA]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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5'-[GpppC]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[GpppG]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[GpppU]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7AraGpppA]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7AraGpppC]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7AraGpppG]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7AraGpppU]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7dGpppA]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7dGpppC]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7dGpppG]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7dGpppU]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GppPAm]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppA]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppCm]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppC]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppGm]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppG]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7Gpppm6Am]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7Gpppm6A]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppUm]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7GpppU]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
5'-[m7Gppp]GUAGAACUUCGUCGAGUACGCUCAA[FAM]-3' + H2O
?
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the substrate is 95% decapped at 0.0027 mM enzyme
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?
7-dimethylguanosine 5'-[3-(5'-guanosinyl)-3-boranotriphosphate] + H2O
7-methylguanosine 5'-phosphate + ?
7-methylguanosin-5'-yl-[3-(5'-guanosinyl)-2-boranotriphosphate] + H2O
7-methylguanosine 5'-phosphate + ?
7-methylguanosine 5'-diphosphate + nucleotidyl 5'-diphosphate + H2O
7-methylguanosine 5'-phosphate + ?
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?
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O
N7-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA]
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the enzyme decaps RNA transcripts as long as 1400 nucleotides
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?
7-methylguanosine 5'-phosphate + ?
boranophosphate analog of the mRNA cap. 90 Min reaction time, 6% cleavage of S-enantiomer, 97% cleavage of R-enantiomer
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?
7-dimethylguanosine 5'-[3-(5'-guanosinyl)-3-boranotriphosphate] + H2O
7-methylguanosine 5'-phosphate + ?
boranophosphate analog of the mRNA cap. 90 Min reaction time, 11% cleavage of S-enantiomer, 98% cleavage of R-enantiomer
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?
7-methylguanosine 5'-phosphate + ?
boranophosphate analog of the mRNA cap. 10 Min reaction time, 90-100% cleavage
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?
7-methylguanosin-5'-yl-[3-(5'-guanosinyl)-2-boranotriphosphate] + H2O
7-methylguanosine 5'-phosphate + ?
boranophosphate analog of the mRNA cap. 90 Min reaction time, 12-19% cleavage
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?
7-methylguanosine 5'-phosphate + phosphate
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?
7-methylguanosine 5'-diphosphate + H2O
7-methylguanosine 5'-phosphate + phosphate
eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA
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?
7-methylguanosine 5'-diphosphate + H2O
7-methylguanosine 5'-phosphate + phosphate
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?
7-methylguanosine 5'-diphosphate + H2O
7-methylguanosine 5'-phosphate + phosphate
cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway
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?
7-methylguanosine 5'-diphosphate + H2O
7-methylguanosine 5'-phosphate + phosphate
mRNA degradation occurs through distinct pathways, one primarily from the 5' end of the mRNA and the second from the 3' end. Decay from the 3' end generates the m7G5'ppp5'N cap dinucleotide, which is subsequently hydrolyzed to m7Gp and nucleotidyl 5'-diphosphate in Saccharomyces cerevisiae by a scavenger decapping activity termed Dcs1p
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?
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids
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m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8, decapping is an important process in the control of eukaryotic mRNA degradation. The scavenger decapping enzyme DcpS functions to clear the cell of cap structure following decay of the RNA body by catalyzing the hydrolysis of m7GpppN to m7Gp and ppN
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8, eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8, DcpS is capable of acting on an mRNA once it is degraded down to 10 nuceotides
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8, the recombinant protein specifically hydrolyzes methylated cap analog but does not hydrolyze unmethylated cap analog nor does it function on intact capped RNA. DcpS is capable of acting on an mRNA once it is degraded down to 10 nucleotides
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
Tyr273 seems to play an important role in cap binding and product release
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8
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?
m7G5'ppp5'N(3'ppp5'N)n + H2O
7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
n = 1-8, mRNA degradation occurs through distinct pathways, one primarily from the 5' end of the mRNA and the second from the 3' end. Decay from the 3' end generates the m7GpppN cap dinucleotide, which is subsequently hydrolyzed to m7Gp and ppN in Saccharomyces cerevisiae by a scavenger decapping activity termed Dcs1p
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?
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Dcps shows no activity with 7-methylguanosine 5'-diphosphate
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additional information
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the enzyme can decap most guanosine caps. In contrast, it shows no activity towards adenosine, cytidine or uridine capped RNAs
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