3.6.1.56: 2-hydroxy-dATP diphosphatase
This is an abbreviated version!
For detailed information about 2-hydroxy-dATP diphosphatase, go to the full flat file.
Word Map on EC 3.6.1.56
-
3.6.1.56
-
mutt
-
triphosphates
-
glycosylase
-
8-oxo-dg
-
sanitization
-
8-oxo-2'-deoxyguanosine
-
8-oxodgtp
-
8-oxo-dgtpase
-
dgtp
-
hnpcc
-
2-oh-datp
-
8-ohdg
-
8-hydroxy-2'-deoxyguanosine
-
medicine
- 3.6.1.56
-
mutt
- triphosphates
- glycosylase
-
8-oxo-dg
-
sanitization
- 8-oxo-2'-deoxyguanosine
- 8-oxodgtp
- 8-oxo-dgtpase
- dgtp
-
hnpcc
-
2-oh-datp
-
8-ohdg
-
8-hydroxy-2'-deoxyguanosine
- medicine
Reaction
Synonyms
(2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase, 8-oxo-dGTP hydrolase, hMTH1, MTH1, MTH2, MutT homologue 1, Nud2, Nudix hydrolase 2, NUDT1
ECTree
3.6.1.56 2-hydroxy-dATP diphosphataseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.6.1.56 - 2-hydroxy-dATP diphosphatase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
8-oxo-GDP + H2O
8-oxo-GMP + phosphate
-
Vmax/Km is 4% of the value for 8-oxo-GTP
-
-
?
2-hydroxy-ATP + H2O
2-hydroxy-AMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy-ATP to monophosphates, and thus avoids errors caused by their misincorporation during DNA replication or transcription, which may result in carcinogenesis or neurodegeneration
-
-
?
2-hydroxy-dATP + 2 H2O
2-hydroxy-dAMP + 2 phosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 protein is an antimutagenic (2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase that prevents the incorporation of oxidatively modified nucleotides into nucleic acids. It decomposes most specifically the miscoding products of oxidative damage to purine nucleic acid precursors (e.g. 8-oxo-dGTP, 2-hydroxy-dATP, 2-hydroxy-ATP, 8-oxo-GTP) that may cause point mutations or transcription errors when incorporated into DNA and RNA, respectively
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
-
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy-ATP to monophosphates, and thus avoids errors caused by their misincorporation during DNA replication or transcription, which may result in carcinogenesis or neurodegeneration
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
MTH1 protects cells from H2O2-induced cell dysfunction and death by hydrolyzing oxidized purine nucleotides including 8-oxo-dGTP and 2-OH-dATP. These alterations may be partly attributed to a mitochondrial dysfunction
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
-
hydrolyzed more efficiently and with higher affinity than 8-oxo-dATP. Preferential hydrolysis of 2-oxo-dATP over 8-oxo-dGTP is observed at all of the pH values tested (pH 7.2 to pH 8.8). A 5fold difference in the hydrolysis efficiencies for 2-oxo-dATP over 8-oxo-dGTP is found at pH 7.2. In addition to the normal form of hMTH1 (valine 83), the variant form of the protein (methionine 83) also hydrolyzes 2-oxo-dATP more efficiently than 8-oxo-dGTP
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
-
is more efficiently hydrolyzed to the monophosphate than is 8-oxo-dGTP
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
is more efficiently hydrolyzed to the monophosphate than is 8-oxo-dGTP
-
-
?
8-oxo-dATP + H2O
8-oxo-dAMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy-ATP to monophosphates, and thus avoids errors caused by their misincorporation during DNA replication or transcription, which may result in carcinogenesis or neurodegeneration
-
-
?
8-oxo-dATP + H2O
8-oxo-dAMP + diphosphate
-
hydrolyzed as efficiently as 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate is produced during cellular metabolism, and its misincorporation into DNA causes mutation. MTH1 hydrolyzes 8-oxo-dGTP to the corresponding nucleoside monophosphate, thereby preventing misincorporation of 8-oxo-7,8-dihydroguanine into DNA. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy-ATP to monophosphates, and thus avoids errors caused by their misincorporation during DNA replication or transcription, which may result in carcinogenesis or neurodegeneration. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
the enzyme is responsible for preventing misincorporation of 8-oxoguanine into DNA. The enzyme protects genetic information from the untoward effects of endogenous oxygen radicals. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP. Trp117 is essential for MTH1 to recognize both 8-oxodGTP and 2-hydroxy-dATP, whereas Asp119 is only essential for recognizing 2-hydroxy-dATP, thus suggesting that origins of the substrate-binding pockets for MTH1 and MutT (from Escherichia coli) are different
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
MTH1 catalyzes hydrolysis of 8-oxo-dGTP through nucleophilic substitution of water at the beta-phosphate. The side chains of Asp119 and Asn33 play an essential role in 2-OH-dATP recognition, whereas the indole ring of Trp117 may be important for interacting with the purine bases of substrate nucleotides. Solution structure of MTH1 solved by multidimensional heteronuclear NMR spectroscopy, identification of the substrate interaction pocket. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP) is formed in the nucleotide pool of a cell during normal cellular metabolism, and when it is incorporated into DNA causes mutation. Organisms possess 8-oxo-dGTPase, an enzyme that specifically degrades 8-oxo-dGTP to 8-oxo-dGMP. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. MTH1 protein is an antimutagenic (2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase that prevents the incorporation of oxidatively modified nucleotides into nucleic acids. It decomposes most specifically the miscoding products of oxidative damage to purine nucleic acid precursors (e.g. 8-oxo-dGTP, 2-hydroxy-dATP, 2-hydroxy-ATP, 8-oxo-GTP) that may cause point mutations or transcription errors when incorporated into DNA and RNA, respectively. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. The MTH2 protein prevents mutations caused by 8-oxoguanine nucleotides. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
-
highest specific activity toward 8-oxo-dGTP
-
-
?
8-oxo-GTP + H2O
8-oxo-GMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate
-
-
?
8-oxo-GTP + H2O
8-oxo-GMP + diphosphate
-
this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-GTP + H2O
8-oxo-GMP + diphosphate
-
the MTH1 protein has the ability to cleave the phosphoanhydride bond between the alpha and the beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP
-
-
?
8-oxo-GTP + H2O
8-oxo-GMP + diphosphate
-
8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate
-
-
?
additional information
?
-
-
no hydrolysis of (R)-8,5'-cyclodATP, 5-oxo-dCTP, and 5-formyl-dUTP
-
-
?
additional information
?
-
-
Vmax/Km for GDP is 0.3% of the value for 8-oxo-GTP
-
-
?
