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3.6.1.45: UDP-sugar diphosphatase

This is an abbreviated version!
For detailed information about UDP-sugar diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.45

Reaction

UDP-sugar
+
H2O
=
UMP
+
alpha-D-aldose 1-phosphate

Synonyms

5'-nucleotidase/UDP-sugar hydrolase, hydrolase, uridine diphosphoglucose (Escherichia coli precursor reduced), hydrolase, uridine diphosphoglucose (Escherichia coli reduced), hydrolase, uridine diphosphoglucose (Salmonella typhimurium clone pAGS5 gene ushB isoenzyme reduced), hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme precursor reduced), hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme reduced), nucleosidediphosphate-sugar diphosphatase, nucleosidediphosphate-sugar pyrophosphatase, Nudix hydrolase-like nucleotide hydrolase, toxin A, toxin B, UDP-glucose pyrophosphatase, UDP-sugar diphosphatase, UDP-sugar hydrolase, UDP-sugar pyrophosphatase, UDPG pyrophosphatase, UGPPase, UshA, UshB protein

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.45 UDP-sugar diphosphatase

Cloned

Cloned on EC 3.6.1.45 - UDP-sugar diphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
from the gene ushA
-
gene UGPP, overexpression in Escherichia coli strain AD494(DE3), subcloning in strain JM109
gene ushA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR
gene ushA, overexpression of the enzyme
-
overexpressed in Corynebacterium glutamicum
-
overexpression of wild-type and mutant UshA enzyme lacking signal peptide in Escherichia coli 5A-1, UshA lacking the signal peptide is an active cytoplasmic 5' nucleotidase which causes conditional lethality
-