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3.6.1.25: triphosphatase

This is an abbreviated version!
For detailed information about triphosphatase, go to the full flat file.

Word Map on EC 3.6.1.25

Reaction

Triphosphate
+
H2O
=
diphosphate
+
phosphate

Synonyms

inorganic triphosphatase, triphosphate tunnel metalloenzyme, tripolyphosphatase, TTM

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.25 triphosphatase

Specific Activity

Specific Activity on EC 3.6.1.25 - triphosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.001
-
R265S mutant, 2 mM methionine and 2 mM ATP as substrate
0.0012
-
tripolyphosphatase activity of R265H mutant, 2 mM ATP as substrate
0.0019
-
tripolyphosphatase activity of R265H mutant, 2 mM pyrophosphate as substrate
0.002
-
R265H mutant, 2 mM methionine and 2 mM ATP as substrate
0.004
-
tripolyphosphatase activity of R265H mutant, 2 mM metatripolyphosphate as substrate
0.029
-
tripolyphosphatase activity of R265S mutant, 2 mM tripolyphosphate as substrate
0.067
-
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM pyrophosphate
0.128
-
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate
0.129
-
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM ATP
0.156
-
tripolyphosphatase activity of wildtype, 2 mM tripolyphosphate as substrate
0.588
-
wildtype, 2 mM methionine and 2 mM ATP as substrate
additional information
-
tripolyphosphate activity of R265H mutant is specific for tripolyphosphate and depends on magnesium ions, potassium is not required