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3.6.1.23: dUTP diphosphatase

This is an abbreviated version!
For detailed information about dUTP diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.23

Reaction

dUTP
+
H2O
=
dUMP
 +
diphosphate

Synonyms

DCD-DUT, dCTP deaminase-dUTPase, dCTP deaminase:dUTPase, deoxyuridine 5'-triphosphate, deoxyuridine 5'-triphosphate nucleotide hydrolase, deoxyuridine 5'-triphosphate nucleotidohydrolase, deoxyuridine 5'-triphosphate pyrophosphatase, deoxyuridine 50-triphosphate nucleotidohydrolase, deoxyuridine 5’-triphosphate nucleotidohydrolase, deoxyuridine nucleotidohydrolase, deoxyuridine triphosphatase, deoxyuridine triphosphate nucleotidohydrolase, deoxyuridine triphosphate pyrophosphatase, deoxyuridine-5'-triphosphate nucleotidohydrolase, deoxyuridine-triphosphate pyrophosphatase, desoxyuridine 5'-triphosphatase, desoxyuridine 5'-triphosphate nucleotidohydrolase, desoxyuridine-triphosphatase, DURP, DUT, DUT-N, DUT1, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPae, dUTPase, dUTPase-dUDPase, dUTPase-related protein, E165R, More, ORF 54, ORF8, P18, PIP4, Pr95, STSV2_30, Tb927.7.5160, U45, YncF, YosS

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.23 dUTP diphosphatase

Purification

Purification on EC 3.6.1.23 - dUTP diphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
large-scale
-
native enzyme from virus
-
Ni-NTA agarose affinity column chromatography and Superose 12 gel filtration
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex S200 gel filtration
Ni-NTA column chromatography and Superose 12 gel filtration
-
nickel affinity resin column chromatography and Superdex-200 gel filtration
partial
-
phosphocellulose column chromatography
phosphocellulose column chromatography and Superdex 200 gel filtration
recombinant C-terminal domain nucleocapsid fusion protein or free enzyme from Escherichia coli by heparin affinity chromatography and gel filtration
-
recombinant enzyme
recombinant enzyme by gel filtration
-
recombinant enzyme by ion exchange chromatography and gel filtration
recombinant enzyme from Escherichia coli by phosphocellulose chromatography and gel filtration to near homogeneity
recombinant enzyme from Escherichia coli strain BL21 by hydroxyapatite and anion exchange chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by cation exchange chromatography, gel filtration, and anion exchange chromatography to near homogeneity
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography, and two steps of anion exchange chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by streptomycin precipitation, anion exchange chromatography, dialysis, and ammonium sulfate fractionation to homogeneity
recombinant enzyme from to homogeneity
recombinant enzyme of Bacillus subtilis temperate bacteriophage SPbeta from Escherichia coli strain BL21(DE3) by phosphocellulose chromatography to homogeneity
Spbetavirus SPbeta
-
recombinant His-tagged enzyme from Escherichia coli by cobalt chelating affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography, cleavage of the tag
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type dUTPase and mutant F158W by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration
-
recombinant N-terminally His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration
-
recombinant protein
recombinant protein using His-tag
recombinant proteins
recombinant wild-type and mutant enzymes from yeast strains
subcellular fractionation
-