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0.00173 - 0.0061
2'-deoxy-5-fluoro-UTP
0.438 - 0.523
beta-L-2'-dUTP
0.0447
dITP
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.0027
dUTP-Co2+
-
pH 7.0, 25°C, recombinant enzyme
0.0019
dUTP-Mg2+
-
pH 7.0, 25°C, recombinant enzyme
0.0137
dUTP-Mn2+
-
pH 7.0, 25°C, recombinant enzyme
additional information
additional information
-
0.00173
2'-deoxy-5-fluoro-UTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0061
2'-deoxy-5-fluoro-UTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.438
beta-L-2'-dUTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.523
beta-L-2'-dUTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0094
dATP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
1.13
dATP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.077
dCTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.465
dCTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.687
dCTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.21
dGTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
1.96
dGTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.11
dTTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.29
dTTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.64
dTTP
-
pH 7.5, 25°C
0.761
dTTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0062
dUDP
-
25°C
0.0627
dUDP
-
pH 8, 25°C
0.0001
dUTP
-
-
0.00017
dUTP
-
pH and temperature not specified in the publication
0.00022
dUTP
-
pH and temperature not specified in the publication
0.0004
dUTP
-
pH 7.5, 25°C
0.0004
dUTP
at pH 7.6 and 25°C
0.0006
dUTP
Spbetavirus SPbeta
-
pH 7.5, 25°C, recombinant enzyme
0.0006
dUTP
mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.00066
dUTP
-
pH 8.0, 25°C, recombinant enzyme
0.0007
dUTP
-
pH 7.6, 25°C, recombinant enzyme, in presence of 1 mM MgCl2
0.00071
dUTP
wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0008
dUTP
wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0009
dUTP
wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C
0.00093
dUTP
-
wild type enzyme, at pH 7.5 and 20°C
0.00096
dUTP
-
pH and temperature not specified in the publication
0.001
dUTP
-
wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.0011
dUTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0011
dUTP
DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C
0.0011
dUTP
mutant enzyme R140K/H145W, at pH 7.5 and 20°C
0.0012
dUTP
-
recombinant enzyme
0.0013
dUTP
mutant enzyme H145W, at pH 7.5 and 20°C
0.0014
dUTP
wild type enzyme, at pH 7.5 and 20°C
0.0015
dUTP
-
in presence of Mg2+, at low concentrations of dUTP
0.0016
dUTP
wild type enzyme, at pH 8.0 and 25°C
0.0017
dUTP
mutant enzyme I117A, at pH 8.0 and 25°C
0.0017
dUTP
wild type enzyme, at pH 7.5 and 20°C
0.00179
dUTP
-
pH 7.4, 37°C, recombinant enzyme
0.0018
dUTP
at pH 7.5 and 20°C
0.0018
dUTP
mutant enzyme F46A, at pH 8.0 and 25°C
0.00187
dUTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0019
dUTP
-
pH 7.0, 25°C, recombinant enzyme
0.0019
dUTP
mutant enzyme K96A, at pH 8.0 and 25°C
0.002
dUTP
mutant enzyme V160D, at pH 7.5 and 20°C
0.0021
dUTP
-
pH 8, 25°C
0.0021
dUTP
mutant enzyme D170G, at pH 7.5 and 20°C
0.0024
dUTP
-
pH 5.7-6.2, recombinant enzyme
0.0025
dUTP
enzyme form DUT-N and DUT-M
0.0025
dUTP
mutant enzyme K150A, at pH 7.5 and 20°C
0.0026
dUTP
mutant enzyme K150E, at pH 7.5 and 20°C
0.0026
dUTP
mutant enzyme R158A, at pH 7.5 and 20°C
0.0026
dUTP
mutant enzyme R158E, at pH 7.5 and 20°C
0.0028
dUTP
mutant enzyme DELTA170-171, at pH 7.5 and 20°C
0.0029
dUTP
mutant enzyme R24T, at pH 7.5 and 20°C
0.0032
dUTP
mutant enzyme R158W, at pH 7.5 and 20°C
0.0036
dUTP
pH 7.4, 20°C, recombinant wild-type enzyme
0.0036
dUTP
-
mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.0037
dUTP
-
in presence of 1 mM Mg2+
0.004
dUTP
-
enzyme from uninfected KB cells
0.004
dUTP
-
in absence of Mg2+, at low concentrations of dUTP
0.004
dUTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.0042
dUTP
mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C
0.0045
dUTP
mutant enzyme S115R, at pH 7.5 and 20°C
0.0048
dUTP
-
enzyme from herpes simplex virus type 1 infected KB cells
0.005
dUTP
mutant enzyme F166Y, at pH 7.5 and 20°C
0.0052
dUTP
mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0052
dUTP
-
mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.0052
dUTP
-
mutant enzyme R166S, at pH 7.5 and 20°C
0.0054
dUTP
mutant enzyme R161K, at pH 7.5 and 20°C
0.006
dUTP
wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0067
dUTP
mutant enzyme R24T/S115R, at pH 7.5 and 20°C
0.0067
dUTP
mutant enzyme T138STOP, at pH 7.5 and 20°C
0.007
dUTP
mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0071
dUTP
mutant enzyme D170L, at pH 7.5 and 20°C
0.0073
dUTP
-
in the presence of 5 mM Mn2+, at pH 8.2 and 25°C
0.008
dUTP
mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0082
dUTP
mutant enzyme G143STOP, at pH 7.5 and 20°C
0.009
dUTP
mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.0117
dUTP
-
pH 8.5, 50°C, recombinant enzyme
0.013
dUTP
mutant enzyme D34N, at pH 7.5 and 20°C
0.013
dUTP
-
mutant enzyme T22R/R116S, at pH 7.5 and 20°C
0.0132
dUTP
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.0142
dUTP
mutant enzyme Q114A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.021
dUTP
mutant enzyme DELTA134-153, at pH 7.5 and 20°C
0.0315
dUTP
mutant enzyme R111A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.041
dUTP
mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.45
dUTP
D90N mutant enzyme, pH 7.8, room temperature
0.5
dUTP
wild-type enzyme, pH 7.8, room temperature
3.788
dUTP
at pH 8.0 and 37°C
0.035
UTP
mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
0.54
UTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.571
UTP
-
at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
additional information
additional information
lower affinity for dUTP than strict dUTPases
-
additional information
additional information
-
lower affinity for dUTP than strict dUTPases
-
additional information
additional information
-
kinetics of dUTP binding
-
additional information
additional information
-
kinetics of dUTP binding
-
additional information
additional information
-
non-cooperative binding to 2'-dUMP, 1 molecule per enzyme subunit, kinetics and thermodynamics from isothermal titration microcalorimetry under different conditions, overview
-
additional information
additional information
detailed pre-steady-state and steady-state kinetic analysis, quantitative kinetic model of the human dUTPase catalytic cycle, overview
-
additional information
additional information
-
detailed pre-steady-state and steady-state kinetic analysis, quantitative kinetic model of the human dUTPase catalytic cycle, overview
-
additional information
additional information
ligand binding constants
-
additional information
additional information
-
ligand binding constants
-
additional information
additional information
steady-state kinetic analysis of the dual activities of the bifunctional enzyme, overview
-
additional information
additional information
-
Michaelis-Menten and ligand binding kinetics, overview. Thermodynamic parameters for the interaction between Plasmodium falciparum dUTPase and deoxyuridine derivatives at 25.2 °C and pH 7.0
-
additional information
additional information
-
Michaelis-Menten kinetics, kinetics analysis
-
additional information
additional information
transient state kinetics of substrate binding to the S72A mutant dUTPase, stopped-flow measurements, overview. Comparative kinetics of formation of the enzyme-substrate complexes of the wild-type and S72A
-
additional information
additional information
-
transient state kinetics of substrate binding to the S72A mutant dUTPase, stopped-flow measurements, overview. Comparative kinetics of formation of the enzyme-substrate complexes of the wild-type and S72A
-