3.6.1.17: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
This is an abbreviated version!
For detailed information about bis(5'-nucleosyl)-tetraphosphatase (asymmetrical), go to the full flat file.
Word Map on EC 3.6.1.17
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3.6.1.17
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nudix
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lupin
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5'-tetraphosphate
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5',5'-p1,p4-tetraphosphate
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mutt
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3.1.4.1
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diphosphoinositol
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angustifolius
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adenosine5'tetraphospho5'adenosine
- 3.6.1.17
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nudix
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lupin
- 5'-tetraphosphate
- 5',5'-p1,p4-tetraphosphate
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mutt
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3.1.4.1
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diphosphoinositol
- angustifolius
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adenosine5'tetraphospho5'adenosine
Reaction
Synonyms
(asymmetrical) diadenosine 5',5''‚-P1,P4-tetraphosphate hydrolase, (asymmetrical) dinucleoside tetraphosphatase, Ap4A hydrolase, Ap4A-hydrolase, AP4AASE, Apf, asym-di-GDPase, asymmetric Ap4A hydrolase, asymmetric diadenosine tetraphosphate hydrolase, asymmetrical Ap4A hydrolase, asymmetrical bis-(5'-nucleosidyl)-tetraphosphatase, asymmetrical diadenosine tetraphosphate hydrolase, AtNUDX25 protein, ATP-producing Ap4A hydrolase, bis(5'-adenosyl)-tetraphosphatase, bis(5'-guanosyl)-tetraphosphatase, bis(5'-nucleosyl)-tetraphosphatase (asymmetrical), bis(5-guanosyl)tetraphosphatase, CT771, Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase, diadenosine 5',5'-P1P4 tetraphosphate hydrolase, diadenosine 5,5-P1,P4-tetraphosphate hydrolase, diadenosine P1,P4-tetraphosphatase, Diadenosine tetraphosphatase, diadenosine tetraphosphate pyrophosphohydrolase, diguanosine tetraphosphatase, diguanosinetetraphosphatase (asymmetrical), diguanosinetetraphosphate guanosylhydrolase, dinucleoside tetraphosphatase, dinucleosidetetraphosphatase (asymmetrical), DIPP1, ENPP4, More, MXAN_1509, NDX-4, nudH, Nudix hydrolase, NUDT2, PFE1035c, PrpA
ECTree
3.6.1.17 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)Advanced search results
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results (Substrates Products
Substrates Products on EC 3.6.1.17 - bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2'-pdA)AppppA + H2O
?
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cleavage to produce ATP and 2'-deoxyadenylated AMP rather than 2'-deoxyadenylated ATP + AMP occurs with a ratio of 60:40 with the wild-type enzyme and the mutant enzymes R54Q, E58D and E125Q. With the mutants R54Q, E58D and E125Q, releasing ATP is increased to 70% of total hydrolysis. Activity of mutants E55Q, E59D and E59Q is to low to be detected
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-
?
2'-deoxyadenosine adenosine tetraphosphate + H2O
ATP + 2'-dAMP + 2'-dATP + AMP
-
-
80% dAMP, 20% AMP
?
5',5''-diadenosine pentaphosphate + H2O
ATP + ADP
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42% of the activity with 5',5''-diadenosine tetraphosphate
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?
5',5'-diadenosine hexaphosphate + H2O
2 ATP
diadenosine hexaphosphate is efficiently hydrolysed to ATP only at pH 7.5 with 20 mM Mg2+
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-
?
A-5'-p-alpha,beta,alpha',beta'-bis(oxymethylene)p-5'-A + H2O
2 alpha,beta-oxymethylene-ADP
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
adenosine 5'-tetraphosphate + AMP
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-
-
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?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
ATP + ADP
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19% of the activity with Ap4A
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-
?
diadenosine 5',5'-P1,P4-tetraphosphate + H2O
ATP + AMP + ?
key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis
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-
?
2-oxo-dATP + H2O
?
less efficient hydrolysis compared to 8-oxo-dGTP
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-
?
2-oxo-dATP + H2O
?
less efficient hydrolysis compared to 8-oxo-dGTP
-
-
?
5',5'-diadenosine tetraphosphate + H2O
ATP + AMP
best substrate
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
-
-
?
8-oxo-dGTP + H2O
8-oxo-dGMP + diphosphate
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
-
-
?
A-5'-p-alpha,beta,alpha',beta'-bis(oxymethylene)p-5'-A + H2O
2 alpha,beta-oxymethylene-ADP
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diadenosine polyphosphate analog
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-
?
A-5'-p-alpha,beta,alpha',beta'-bis(oxymethylene)p-5'-A + H2O
2 alpha,beta-oxymethylene-ADP
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diadenosine polyphosphate analog
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-
?
betabeta'-methyleneoxy-Ap4A + H2O
AMP + beta,gamma-methyleneoxy-ATP
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diadenosine polyphosphate analog
-
-
?
betabeta'-methyleneoxy-Ap4A + H2O
AMP + beta,gamma-methyleneoxy-ATP
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diadenosine polyphosphate analog
-
-
?
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
AMP + ATP
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-
-
?
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
AMP + ATP
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
ATP + AMP
-
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
ATP + AMP
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-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ATP + AMP
best substrate
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-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ATP + AMP
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possible role as reporter for luciferase gene
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?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ATP + AMP
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-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ATP + AMP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ATP + AMP
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stress response
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?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
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in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes Ap4A into two molecules of ADP, reaction of EC 3.6.1.41
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?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
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in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes Ap4A into two molecules of ADP, reaction of EC 3.6.1.41
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?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
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?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
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?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
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?
additional information
?
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enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products
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?
additional information
?
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enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products
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-
?
additional information
?
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Apf always produces an NTP product, with substrate preference depending on pH and divalent ion
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?
additional information
?
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Apf always produces an NTP product, with substrate preference depending on pH and divalent ion
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?
additional information
?
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enzyme is a diphosphoinositol polyphosphate phosphohydrolase, EC 3.6.1.52
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-
?
additional information
?
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PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
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?
additional information
?
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PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
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?
