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3.6.1.17: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)

This is an abbreviated version!
For detailed information about bis(5'-nucleosyl)-tetraphosphatase (asymmetrical), go to the full flat file.

Word Map on EC 3.6.1.17

Reaction

P1,P4-bis(5'-guanosyl) tetraphosphate
+
H2O
=
GTP
+
GMP

Synonyms

(asymmetrical) diadenosine 5',5''‚-P1,P4-tetraphosphate hydrolase, (asymmetrical) dinucleoside tetraphosphatase, Ap4A hydrolase, Ap4A-hydrolase, AP4AASE, Apf, asym-di-GDPase, asymmetric Ap4A hydrolase, asymmetric diadenosine tetraphosphate hydrolase, asymmetrical Ap4A hydrolase, asymmetrical bis-(5'-nucleosidyl)-tetraphosphatase, asymmetrical diadenosine tetraphosphate hydrolase, AtNUDX25 protein, ATP-producing Ap4A hydrolase, bis(5'-adenosyl)-tetraphosphatase, bis(5'-guanosyl)-tetraphosphatase, bis(5'-nucleosyl)-tetraphosphatase (asymmetrical), bis(5-guanosyl)tetraphosphatase, CT771, Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase, diadenosine 5',5'-P1P4 tetraphosphate hydrolase, diadenosine 5,5-P1,P4-tetraphosphate hydrolase, diadenosine P1,P4-tetraphosphatase, Diadenosine tetraphosphatase, diadenosine tetraphosphate pyrophosphohydrolase, diguanosine tetraphosphatase, diguanosinetetraphosphatase (asymmetrical), diguanosinetetraphosphate guanosylhydrolase, dinucleoside tetraphosphatase, dinucleosidetetraphosphatase (asymmetrical), DIPP1, ENPP4, More, MXAN_1509, NDX-4, nudH, Nudix hydrolase, NUDT2, PFE1035c, PrpA

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.17 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)

Cloned

Cloned on EC 3.6.1.17 - bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged Apf in Spodoptera frugiperda Sf21 cells, expression of EGFP-tagged enzyme in Drosophila melanbogaster Mel-2 cells
expressed in Escherichia coli
Lupinus sp.
-
expressed in Escherichia coli BL21(DE3)T cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
expression in Escherichia coli
expression in Escherichia coli, mutant enzymes Y27A, Y27D, H31A, H31V, W32G, K36M, H38G, H38K, E52Q, E56Q, Y76A, Y76/Y121A, K79M, K81M, K83M, E103Q, Y121A
-
expression in insect cells
fused to glutathione S-transferase and expressed in E. coli
overexpression in Escherichia coli