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0.045
2''-O-acetyl-ADP-ribose
-
at pH 7.5 and 37°C
0.0035 - 0.0038
8-oxo-dGDP
0.0209 - 0.3213
ADP-D-ribose
3.16
ADP-glucose
-
25°C, pH 7.6, wild-type enzyme
0.083 - 0.154
ADP-mannose
0.46
ADPribose 2'-phosphate
-
-
3.9 - 31
CDP-ethanolamine
1.36
CDP-ribose
-
25°C, pH 7.6, wild-type enzyme
1.1
GDP-ribose
-
25°C, pH 7.6, wild-type enzyme
additional information
additional information
-
0.76
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
1.05
2',3'-cAMP
mutant N110A, pH 7.5, 37°C
1.25
2',3'-cAMP
mutant H111A, pH 7.5, 37°C
1.7
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
1.8
2',3'-cAMP
mutant H111N, pH 7.5, 37°C
2.3
2',3'-cAMP
mutant Q27H, pH 7.5, 37°C
2.4
2',3'-cAMP
wild-type, pH 7.5, 37°C
2.5
2',3'-cAMP
mutant F37Y, pH 7.5, 37°C
2.6
2',3'-cAMP
mutant C253A, pH 7.5, 37°C
3.4
2',3'-cAMP
mutant L196A, pH 7.5, 37°C
4.8
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37°C
5.1
2',3'-cAMP
mutant F37A, pH 7.5, 37°C
5.2
2',3'-cAMP
mutant F37A/L196F/C253A, pH 7.5, 37°C
7.1
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37°C
7.6
2',3'-cAMP
mutant F210A, pH 7.5, 37°C
7.6
2',3'-cAMP
mutant R43A, pH 7.5, 37°C
0.0035
8-oxo-dGDP
at pH 10.0 and 37°C
0.0038
8-oxo-dGDP
at pH 8.0 and 37°C
2.65
ADP
wild-type, pH 7.5, temperature not specified in the publication
19
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
0.0209
ADP-D-ribose
mutant enzyme D164N, at pH 7.0 and 37°C
0.0223
ADP-D-ribose
wild type enzyme, at pH 7.0 and 37°C
0.0231
ADP-D-ribose
mutant enzyme E166Q, at pH 7.0 and 37°C
0.0257
ADP-D-ribose
mutant enzyme E116Q, at pH 7.0 and 37°C
0.0279
ADP-D-ribose
mutant enzyme R111Q, at pH 7.0 and 37°C
0.0389
ADP-D-ribose
mutant enzyme E93Q, at pH 7.0 and 37°C
0.0402
ADP-D-ribose
mutant enzyme E115Q, at pH 7.0 and 37°C
0.0434
ADP-D-ribose
mutant enzyme D164A, at pH 7.0 and 37°C
0.0456
ADP-D-ribose
mutant enzyme D133N, at pH 7.0 and 37°C
0.0463
ADP-D-ribose
mutant enzyme C139A, at pH 7.0 and 37°C
0.048
ADP-D-ribose
-
at pH 7.5 and 37°C
0.0702
ADP-D-ribose
mutant enzyme E112Q, at pH 7.0 and 37°C
0.0879
ADP-D-ribose
mutant enzyme D133A, at pH 7.0 and 37°C
0.1084
ADP-D-ribose
mutant enzyme R84Q, at pH 7.0 and 37°C
0.1197
ADP-D-ribose
mutant enzyme R196Q, at pH 7.0 and 37°C
0.1265
ADP-D-ribose
mutant enzyme W46A, at pH 7.0 and 37°C
0.1271
ADP-D-ribose
mutant enzyme Q82A, at pH 7.0 and 37°C
0.1283
ADP-D-ribose
mutant enzyme L98A, at pH 7.0 and 37°C
0.1497
ADP-D-ribose
mutant enzyme W28A/W46A, at pH 7.0 and 37°C
0.184
ADP-D-ribose
mutant enzyme W28A, at pH 7.0 and 37°C
0.3213
ADP-D-ribose
mutant enzyme R51Q, at pH 7.0 and 37°C
0.083
ADP-mannose
pH 7.0, 37°C
0.154
ADP-mannose
-
pH 9.0, 37°C
0.0019
ADP-ribose
at pH 8.0 and 37°C
0.0169
ADP-ribose
-
37°C, pH 8.0, AtNUDT2
0.018
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E70Q
0.0223
ADP-ribose
pH 7.0, 37°C, wild-type enzyme
0.023
ADP-ribose
-
37°C, pH 8.0, AtNUDT6
0.0232
ADP-ribose
-
37°C, pH 8.0, AtNUDT7
0.027
ADP-ribose
-
25°C, pH 7.6, mutant enzyme R81Q
0.0274
ADP-ribose
-
37°C, pH 8.0, AtNUDT10
0.032
ADP-ribose
pH 7.0, 37°C
0.036
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E73Q
0.036
ADP-ribose
at pH 10.0 and 37°C
0.038
ADP-ribose
-
pH 9.0, 37°C
0.043
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E85Q
0.05
ADP-ribose
-
25°C, pH 7.6, mutant enzyme T155A
0.053
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
0.06
ADP-ribose
pH 7.5, 37°C
0.06
ADP-ribose
wild-type, pH 7.5, 37°C
0.06
ADP-ribose
-
25°C, pH 7.6, mutant enzyme T110A
0.065
ADP-ribose
-
37°C, pH 8.0, ADP-ribose pyrophosphatase Slr0920
0.071
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
0.077
ADP-ribose
mutant F37Y, pH 7.5, 37°C
0.078
ADP-ribose
mutant H111N, pH 7.5, 37°C
0.09
ADP-ribose
-
25°C, pH 7.6, mutant enzyme Y99F
0.094
ADP-ribose
mutant C253A, pH 7.5, 37°C
0.1
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E86Q
0.1
ADP-ribose
-
25°C, pH 7.6, mutant enzyme S153A
0.11
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E82Q
0.11
ADP-ribose
-
25°C, pH 7.6, wild-type enzyme
0.11
ADP-ribose
-
37°C, pH 8.0, ADP-ribose pyrophosphatase Sll1054
0.115
ADP-ribose
mutant R43A, pH 7.5, 37°C
0.12
ADP-ribose
-
25°C, pH 7.6, mutant enzyme R18Q
0.13
ADP-ribose
mutant L196A, pH 7.5, 37°C
0.14
ADP-ribose
-
25°C, pH 7.6, mutant enzyme R27Q
0.14
ADP-ribose
mutant H111A, pH 7.5, 37°C
0.2
ADP-ribose
mutant Q27H, pH 7.5, 37°C
0.204
ADP-ribose
-
pH 8.0
0.24
ADP-ribose
-
25°C, pH 7.6, mutant enzyme Q52A
0.25
ADP-ribose
pH 9.0, 25°C
0.29
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E108Q
0.29
ADP-ribose
-
37°C, pH 8.0, ADP-ribose pyrophosphatase Slr1134
0.29
ADP-ribose
mutant N110A, pH 7.5, 37°C
0.31
ADP-ribose
-
25°C, pH 7.6, mutant enzyme L68A
0.4
ADP-ribose
-
25°C, pH 7.6, mutant enzyme E63Q
0.4
ADP-ribose
-
25°C, pH 7.6, mutant enzyme Y28Q
0.42
ADP-ribose
-
25°C, pH 7.6, mutant enzyme H33A
0.52
ADP-ribose
pH 7.5, 25°C
0.65
ADP-ribose
-
25°C, pH 7.6, mutant enzyme R54Q
0.69
ADP-ribose
-
25°C, pH 7.6, mutant enzyme S102A
1.15
ADP-ribose
mutant F37A, pH 7.5, 37°C
1.15
ADP-ribose
mutant F37A/L196A, pH 7.5, 37°C
1.2
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37°C
1.24
ADP-ribose
-
25°C, pH 7.6, mutant enzyme I19A
1.6
ADP-ribose
mutant F37A/L196F, pH 7.5, 37°C
2.1
ADP-ribose
mutant F210A, pH 7.5, 37°C
0.0004
ADPribose
-
pH 7.5, 37°C
0.0005 - 0.001
ADPribose
-
ADPRibase I
0.002 - 0.003
ADPribose
-
-
0.015 - 0.03
ADPribose
-
ADPRibase-Mn
0.031
ADPribose
-
mutant enzyme D126N, in presence of Mg2+
0.044
ADPribose
-
mutant enzyme E129Q, in presence of Mg2+
0.05 - 0.1
ADPribose
-
ADPRibase II
0.058
ADPribose
-
mutant enzyme D128N, in presence of Mg2+
0.071
ADPribose
-
mutant enzyme E127Q, in presence of Mg2+
0.09
ADPribose
-
mutant enzyme E86Q, in presence of Mg2+
0.094
ADPribose
pH 9.5, 37°C
0.11
ADPribose
-
wild-type enzyme, in presence of Mg2+
0.215
ADPribose
-
mutant enzyme E82Q, in presence of Zn2+
0.325
ADPribose
-
mutant enzyme E86Q, in presence of Zn2+
0.36
ADPribose
-
mutant enzyme E82Q, in presence of Mg2+
0.37
ADPribose
-
wild-type enzyme, in presence of Zn2+
0.19
cADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
0.2
cADP-ribose
mutant C253A, pH 7.5, 37°C
0.46
cADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37°C
0.78
cADP-ribose
wild-type, pH 7.5, 37°C
0.35
CDP-choline
wild-type, pH 7.5, 37°C
0.43
CDP-choline
mutant F37Y, pH 7.5, 37°C
0.47
CDP-choline
mutant L196A, pH 7.5, 37°C
0.5
CDP-choline
mutant C253A, pH 7.5, 37°C
0.97
CDP-choline
mutant F37A, pH 7.5, 37°C
1.25
CDP-choline
mutant F37A/L196A, pH 7.5, 37°C
1.5
CDP-choline
mutant F37A/L196F, pH 7.5, 37°C
1.7
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37°C
2.7
CDP-choline
mutant H111N, pH 7.5, 37°C
2.7
CDP-choline
mutant Q27H, pH 7.5, 37°C
4
CDP-choline
mutant N110A, pH 7.5, 37°C
7.5
CDP-choline
mutant H111A, pH 7.5, 37°C
9
CDP-choline
mutant R43A, pH 7.5, 37°C
11
CDP-choline
mutant F210A, pH 7.5, 37°C
12
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
43
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
3.9
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
31
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
2
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
6.3
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
0.3
FAD
pH 7.5, 25°C
additional information
additional information
-
-
-
additional information
additional information
-
effect of N-acetyl-p-benzoquinoneimine on Km
-
additional information
additional information
-
kinetic analysis, Trp28 and Trp46 function synergistically in substrate binding and catalysis, overview
-
additional information
additional information
kinetic analysis, Trp28 and Trp46 function synergistically in substrate binding and catalysis, overview
-
3.6.1.13 ADP-ribose diphosphatase
results (
results (
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