3.6.1.11: exopolyphosphatase
This is an abbreviated version!
For detailed information about exopolyphosphatase, go to the full flat file.
Word Map on EC 3.6.1.11
Reaction
Synonyms
40 kD exopolyphosphatase, 40-kDa-exopolyphosphatase, acid phosphoanhydride phosphohydrolase, CJJ81176_0377, CJJ81176_1251, CT0099, CT1713, ecPpx, exopoly(P)ase, ExopolyPase, exopolyphosphatase, exopolyphosphatase 1, exopolyphosphatase 2, Gra-Pase, h-prune, high molecular mass exopolyphosphatase, high molecular weight exopolyphosphatase, high-molecular exopolyphosphatase, LmPPX, major cytosolic exopolyphosphatase PPX1, membrane-bound exopolyphosphatase, metaphosphatase, More, Msed_0981, MT0516, NMB1467, nuclear exopolyphosphatase, Nudix hydrolase, paPpx, phosphatase, exopoly-, polyphosphate phosphatase, polyphosphate phosphohydrolase, polyphosphate-phosphohydrolase, Ppn1, PPX, Ppx protein, Ppx/GppA phosphatase, PPX1, PPX2, PPXI, PPXMsed, Rv0496, Tb927.5.4350, Tb927.6.2670, TbDcp2, TbNH2, TbNH4, vacuolar exopolyphosphatase, YHR201C, Za10_0559, ZmPPX
ECTree
3.6.1.11 exopolyphosphataseAdvanced search results
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results (Storage Stability
Storage Stability on EC 3.6.1.11 - exopolyphosphatase
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STORAGE STABILITY 
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM Tris-HCl buffer, pH 7.6, 10 mM MgCl2, 0.5 mM EDTA, 150 mM NaCl, 20% loss of activity after 2 months
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-20°C, in 50 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl2, 0.5 mM EDTA, 50 mM NaCl, 0.1% w/v bovine serum albumin, 40% v/v glycerol, exopolyphosphatase I and II, less than 30% loss of activity after 2 months
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-4°C, enzyme desorbed from heparin-agarose with 1 M KCl and 0.1% Triton X-100, 4 days, no loss of activity
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-80°C, 300 mM NaCl, 250 mM sucrose, 2 mM dithiothreitiol, several months, no activity loss
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4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
4°C, enzyme obtained by desorption with polyphosphate, 2 h, 30% loss of activity
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4°C, in absence of detergents, complete loss of activity after 24 h, can be stabilized by 0.1% Triton X-100 and protease inhibitors
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92°C, in the presence of 0.1% Triton X-100, 5 days, 8% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography
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92°C, without 0.1% Triton X-100, 5 days, 66% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography
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