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3.6.1.11: exopolyphosphatase

This is an abbreviated version!
For detailed information about exopolyphosphatase, go to the full flat file.

Word Map on EC 3.6.1.11

Reaction

(Polyphosphate)n
+
H2O
=
(polyphosphate)n-1
+
phosphate

Synonyms

40 kD exopolyphosphatase, 40-kDa-exopolyphosphatase, acid phosphoanhydride phosphohydrolase, CJJ81176_0377, CJJ81176_1251, CT0099, CT1713, ecPpx, exopoly(P)ase, ExopolyPase, exopolyphosphatase, exopolyphosphatase 1, exopolyphosphatase 2, Gra-Pase, h-prune, high molecular mass exopolyphosphatase, high molecular weight exopolyphosphatase, high-molecular exopolyphosphatase, LmPPX, major cytosolic exopolyphosphatase PPX1, membrane-bound exopolyphosphatase, metaphosphatase, More, Msed_0981, MT0516, NMB1467, nuclear exopolyphosphatase, Nudix hydrolase, paPpx, phosphatase, exopoly-, polyphosphate phosphatase, polyphosphate phosphohydrolase, polyphosphate-phosphohydrolase, Ppn1, PPX, Ppx protein, Ppx/GppA phosphatase, PPX1, PPX2, PPXI, PPXMsed, Rv0496, Tb927.5.4350, Tb927.6.2670, TbDcp2, TbNH2, TbNH4, vacuolar exopolyphosphatase, YHR201C, Za10_0559, ZmPPX

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.11 exopolyphosphatase

Specific Activity

Specific Activity on EC 3.6.1.11 - exopolyphosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.006
-
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium
0.01
-
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium
0.014
-
40-kDa-exopolyphosphatase from Saccharomyces cerevisiae strain CRN grown on glucose
0.034
0.035
-
high-molecular weight exopolyphosphatase from Saccharomyces cerevisiae strain CRX grown on glucose
0.05
1.25 mM polyphosphate 3 as substrate, in the presence of 2.5 mM Mg2+
0.055
-
strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium
0.058
-
soluble form of exopolyphospatase from Saccharomyces cerevisiae strain CRX grown on lactate
0.07
-
strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium
0.095
0.097
-
membrane-bound exopolyphosphatase from Saccharomyces cerevisiae strain CRY grown on glucose
0.119
-
soluble form of exopolyphospatase from Saccharomyces cerevisiae strain CRY grown on lactate
0.12
wild type, samples containing 50 mM PIPES, pH 6.8, 25 mM KCl, and 2 mM MgCl2
0.136
-
40-kDa-exopolyphosphatase from Saccharomyces cerevisiae strain CRY grown on glucose
0.18
-
parent strain CRY, cytosol preparation in exponential growth phase in phosphate-rich medium
0.21
0.13 mM polyphosphate 15 as substrate, in the presence of 1 mM Co2+
0.23
0.13 mM polyphosphate 15 as substrate, in the presence of 0.1 mM Co2+
0.28
0.01 mM polyphosphate 208 as substrate, in the presence of 1 mM Co2+
0.3
2.0 mM polyphosphate 208 as substrate, in the presence of 0.1 mM Co2+
0.43
overexpression of ppx1 increase the specific activity of exopolyphosphatase by 4fold compared to that of the empty vector control
0.47
-
enzyme from cystosol
0.8
crude cell extracts of wild type strain pVWEx1-ppx2 show 6fold higher exopolyphosphatase activity than those of wild type pVWEx1
1.11
-
after ammonium sulfate precipitation
1.26
-
mitochondrial fraction
10
+/- 0.61, 5'-AMP
12.1
+/- 0.90, 3'-CMP
126
substrate (phosphate)300, pH 6.5, 25°C
15
substrate (phosphate)300, pH 6.5, 25°C
16.4
+/- 0.80, 5'-dGMP
170
substrate (phosphate)13-18, pH 6.5, 25°C
180
substrate (phosphate)13-18, pH 6.5, 25°C
20.1
+/- 0.76, 3'-AMP
2070
-
purified recombinant enzyme
22.4
+/- 1.78, 5'-GMP
290
substrate (phosphate)208, presence of 0.1 mM Co2+, pH 7.2, 30°C
3 - 8
substrate (phosphate)3, presence of 0.1 mM Co2+, pH 7.2, 30°C
5
-
after DEAE-Toyopearl 650 M column chromatography
590
substrate (phosphate)3, pH 6.5, 25°C
7.24
+/- 0.34, p-nitrophenyl phosphate
additional information