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0.006
-
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium
0.01
-
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium
0.014
-
40-kDa-exopolyphosphatase from Saccharomyces cerevisiae strain CRN grown on glucose
0.035
-
high-molecular weight exopolyphosphatase from Saccharomyces cerevisiae strain CRX grown on glucose
0.05
1.25 mM polyphosphate 3 as substrate, in the presence of 2.5 mM Mg2+
0.055
-
strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium
0.058
-
soluble form of exopolyphospatase from Saccharomyces cerevisiae strain CRX grown on lactate
0.07
-
strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium
0.097
-
membrane-bound exopolyphosphatase from Saccharomyces cerevisiae strain CRY grown on glucose
0.119
-
soluble form of exopolyphospatase from Saccharomyces cerevisiae strain CRY grown on lactate
0.12
wild type, samples containing 50 mM PIPES, pH 6.8, 25 mM KCl, and 2 mM MgCl2
0.136
-
40-kDa-exopolyphosphatase from Saccharomyces cerevisiae strain CRY grown on glucose
0.18
-
parent strain CRY, cytosol preparation in exponential growth phase in phosphate-rich medium
0.21
0.13 mM polyphosphate 15 as substrate, in the presence of 1 mM Co2+
0.23
0.13 mM polyphosphate 15 as substrate, in the presence of 0.1 mM Co2+
0.28
0.01 mM polyphosphate 208 as substrate, in the presence of 1 mM Co2+
0.3
2.0 mM polyphosphate 208 as substrate, in the presence of 0.1 mM Co2+
0.43
overexpression of ppx1 increase the specific activity of exopolyphosphatase by 4fold compared to that of the empty vector control
0.47
-
enzyme from cystosol
0.8
crude cell extracts of wild type strain pVWEx1-ppx2 show 6fold higher exopolyphosphatase activity than those of wild type pVWEx1
1.11
-
after ammonium sulfate precipitation
1.26
-
mitochondrial fraction
126
substrate (phosphate)300, pH 6.5, 25°C
15
substrate (phosphate)300, pH 6.5, 25°C
170
substrate (phosphate)13-18, pH 6.5, 25°C
180
substrate (phosphate)13-18, pH 6.5, 25°C
2070
-
purified recombinant enzyme
290
substrate (phosphate)208, presence of 0.1 mM Co2+, pH 7.2, 30°C
3 - 8
substrate (phosphate)3, presence of 0.1 mM Co2+, pH 7.2, 30°C
5
-
after DEAE-Toyopearl 650 M column chromatography
590
substrate (phosphate)3, pH 6.5, 25°C
7.24
+/- 0.34, p-nitrophenyl phosphate
0.034
-
membrane-bound form of exopolyphospatase from Saccharomyces cerevisiae strain CRX grown on lactate
0.034
-
membrane-bound form of exopolyphospatase from Saccharomyces cerevisiae strain CRY grown on lactate
0.04
analysis of the constructed deletion mutant ppx2 reveal reduced exopolyphosphatase activity, samples containing 50 mM PIPES, pH 6.8, 25 mM KCl, and 2 mM MgCl2
0.04
1.25 mM diphosphate as substrate, in the presence of 2.5 mM Mg2+
0.04
-
strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium
0.08
analysis of the constructed deletion mutant ppx1 reveal reduced exopolyphosphatase activity, samples containing 50 mM PIPES, pH 6.8, 25 mM KCl, and 2 mM MgCl2
0.08
-
membrane-bound exopolyphosphatase from Saccharomyces cerevisiae strain CRX grown on glucose
0.095
0.01 mM polyphosphate 208 as substrate, in the presence of 2.5 mM Mg2+
0.095
-
strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium
0.1
+/- 0.01, polyphosphate
0.1
0.13 mM polyphosphate 15 as substrate, in the presence of 2.5 mM Mg2+
0.1
-
parent strain CRY, cytosol preparation in exponential growth phase in phosphate-deficient medium
150
-
pH 7.2, 30°C, polyP208 as substrate
150
-
after 319fold purification with heparin agarose, at 30°C in 1 ml of reaction mixture containing 50 mM Tris-HCl buffer, pH 7.2, 0.1 mM CoSO4, 200 mM ammonium chloride, and 0.01 mM polyphosphate 208
240
substrate (phosphate)15, presence of 0.1 mM Co2+, pH 7.2, 30°C
240
purified recombinant enzyme, pH 7.2, 30°C
additional information
when various short- to medium-chain PolyPs are tested, His-tagged PPX2 is most active with polyphosphates of 3-20 phosphate residues
additional information
when various short- to medium-chain PolyPs are tested, His-tagged PPX2 is most active with polyphosphates of 3-20 phosphate residues
additional information
-
when various short- to medium-chain PolyPs are tested, His-tagged PPX2 is most active with polyphosphates of 3-20 phosphate residues
additional information
-
-
additional information
-
h-prune efficiently hydrolyzes short-chain polyphosphates, including inorganic tripoly- and tetrapolyphosphates and nucleoside 5'-tetraphosphates, long-chain inorganic polyphosphates (more than 25 phosphate residues) are converted more slowly
additional information
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increasing amounts of total RNA extracted from eggs progressively enhance nuclear PPX activity, whereas it exerts no effect on mitochondrial PPX activity, nuclear PPX activity increases throughout embryogenesis
additional information
-
-
additional information
-
-
additional information
-
the exopolyphosphatase activity is reduced 6.5fold in the mutant CRN lacking endopolyphosphatase activity
additional information
-
no specific activity on diphosphate, ATP and p-nitrophenyl phosphate
additional information
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polyphosphate levels in different cell compartments, overview
additional information
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polyphosphate hydrolysis in the CRX strain cytosol completes in 120 min