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3.6.1.11: exopolyphosphatase

This is an abbreviated version!
For detailed information about exopolyphosphatase, go to the full flat file.

Word Map on EC 3.6.1.11

Reaction

(Polyphosphate)n
+
H2O
=
(polyphosphate)n-1
 +
phosphate

Synonyms

40 kD exopolyphosphatase, 40-kDa-exopolyphosphatase, acid phosphoanhydride phosphohydrolase, CJJ81176_0377, CJJ81176_1251, CT0099, CT1713, ecPpx, exopoly(P)ase, ExopolyPase, exopolyphosphatase, exopolyphosphatase 1, exopolyphosphatase 2, Gra-Pase, h-prune, high molecular mass exopolyphosphatase, high molecular weight exopolyphosphatase, high-molecular exopolyphosphatase, LmPPX, major cytosolic exopolyphosphatase PPX1, membrane-bound exopolyphosphatase, metaphosphatase, More, Msed_0981, MT0516, NMB1467, nuclear exopolyphosphatase, Nudix hydrolase, paPpx, phosphatase, exopoly-, polyphosphate phosphatase, polyphosphate phosphohydrolase, polyphosphate-phosphohydrolase, Ppn1, PPX, Ppx protein, Ppx/GppA phosphatase, PPX1, PPX2, PPXI, PPXMsed, Rv0496, Tb927.5.4350, Tb927.6.2670, TbDcp2, TbNH2, TbNH4, vacuolar exopolyphosphatase, YHR201C, Za10_0559, ZmPPX

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.11 exopolyphosphatase

Purification

Purification on EC 3.6.1.11 - exopolyphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by metal chelate chromatography
-
DEAE Sepharose filtration
-
DEAE-Toyopearl 650 M column chromatography
-
exopolyphosphatase I and II
-
gel filtration
-
glutathione-Sepharose resin column chromatography or amylose-resin column chromatography
HP-SP-Sepharose column chromatography
-
ion-exchange chromatography
isolation of a recombinant His-tagged protein by affinity chromatography followed by cleavage of the polyhistidine tag
-
isolation of two isoenzymes by cellular fractionation and gel filtration
-
partial, using ion-exchange chromatography and gel filtration
-
recombinant enzyme PPX1 from Saccharomyces cerevisiae strain CRN by ammonium sulfate fractionation, anion exchange chromatography, heparin affinity chromatography, all alternating with ultrafiltration steps
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-Gami B (DE3)pLysS by nickel affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration, followed by cleavage of the His tag by PreScission protease, and gel filtration
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus by nickel affinity chromatography, dialysis, tag cleavage thriugh thrombin, and again dialysis
-
recombinant PPX1 17.4fold in a two-step procedure
-
recombinant ppx2 enzyme is purified by Ni-nitrilotriacetic acid chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis