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3.6.1.10: endopolyphosphatase

This is an abbreviated version!
For detailed information about endopolyphosphatase, go to the full flat file.

Word Map on EC 3.6.1.10

Reaction

Polyphosphate
+ n H2O = (n+1) oligophosphate

Synonyms

DDP1, dual exo/endopolyphoshatase, endopolyPase, endopolyphosphatase, metaphosphatase, More, Nudix hydrolase, phosphatase, endopoly-, polymetaphosphatase, polyphosphatase, polyphosphate depolymerase, PPN, Ppn1, Ppn2, short-chain polyphosphate, Tb927.6.2670, TbDcp2, TbNH4, YDR452W, YNL217W, YOR163W

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.10 endopolyphosphatase

Cloned

Cloned on EC 3.6.1.10 - endopolyphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
gene ddp1, recombinant overexpression in Saccharomyces cerevisiae strain CRN
gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview
gene ppn1, DNA and amino acid sequence determination and analysis, expression of wild-type pre-Ppn1 and mutants in Escherichia coli and in HEK-293 cells
gene ppn1, recombinant overexpression in Saccharomyces cerevisiae strain CRN
gene ppn2, recombinant overexpression of the soluble enzyme in Saccharomyces cerevisiae strain CRN
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)