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3.6.1.1: inorganic diphosphatase

This is an abbreviated version!
For detailed information about inorganic diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.1

Reaction

diphosphate
+
H2O
= 2 phosphate

Synonyms

acid PPase, AT727_13205, AtPPA1, AtPPsPase1, BT2127, CBS-PPase, CBS-PPase1, chloroplast inorganic pyrophosphatase 1, class A type PPA, cobalt-dependent inorganic pyrophosphatase, CPE2055, cytosolic PPase, D1C enzyme, dhPPase, E-PPase, EcPpA, EcPPiase, ehPPase, family I inorganic pyrophosphatase, family I PPase, family I pyrophosphatase, family II inorganic pyrophosphatase, family II PPase, H+-PPase, H+-pyrophosphatase, H+-translocating/vacuolar inorganic pyrophosphatase, Hpp1 protein, HVO_0729, HvPPA, hyperthermophilic inorganic pyrophosphatase, inorganic diphosphatase, inorganic PPase, inorganic pyrophosphatase, inorganic pyrophosphatase 1, inorganic pyrophosphatase-like protein, inorganic pyrophosphorylase, IPP, IPP1, Ipp1p, IPPase, LHPPase, M-PPase, manganese-dependent inorganic pyrophosphatase, MazG, MdPPa, membrane-integral inorganic pyrophosphatase, membrane-integral pyrophosphatase, miPPase, mitochondrial pyrophosphatase, Mn2+-bound canonical Family II PPase, More, Mtb PPiase, Mthe_0236, MtPPA1, MtPPiase, nucleotide-regulated pyrophosphatase, OVP1, p26.1a, p26.1b, PfPPase, PH1907, Pho-PPase, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, phosphoprotein p26.1, PPA, PPA1, PpaC, PPase, PPase 1, PPase 2, ppase-1, PPase-2, PPase1, PPase2, PPase4, PPias, PPIase, proton-translocating inorganic pyrophosphatase, PYP-1, pyrophosphatase, pyrophosphatase, inorganic, Pyrophosphate phospho-hydrolase, pyrophosphate phospho-hydrolase 1, Pyrophosphate phosphohydrolase, Pyrophosphate-energized inorganic pyrophosphatase, RmPPase, Rv3628, siPPiase, soluble inorganic pyrophosphatase, soluble inorganic pyrophosphatase 2, soluble pyrophosphatase, soluble-type yeast PPase, sPPAse, ST2226, Tc-sPPase, TC004566, TcasGA2_TC004566, TgPPase, ThPP1, Tm-PPase, TM0913, V-PPase, vacuolar H(+)-PPase, vacuolar H(+)-pyrophosphatase, vacuolar H+-pyrophosphatase, vacuolar H+-translocating inorganic pyrophosphatase, vacuolar H+-translocating pyrophosphatase, vacuolar pyrophosphatase, VHP, VP1, VSP, VVPP1

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.1 inorganic diphosphatase

Purification

Purification on EC 3.6.1.1 - inorganic diphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and anion exchange column chromatography
-
ammonium sulfate precipitation and Hi-Trap Q column chromatography
-
DEAE-Sepharose column chromatography
-
native enzyme
native enzyme from germinated seeds by acetone precipitation, gel ifltration, and anion exchange chromatography, 51.7fold
-
native enzyme from muscles, 114.5fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, and gel filtration to homogeneity
-
native enzyme from seedlings 30fold by acetone precipitation, gel filtration, and anion exchange chromatography
-
native enzyme, and recombinant enzyme from Escherichia coli strain XL1-blue
native isozyme sPPase-I by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration
native isozyme sPPase-II by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration
Ni-IMAC HisTrap column chromatography and S-200 gel filtration
Ni-NTA column chromatography
Ni2+-charged resin column chromatography and gel filtration
-
nickel affinity column chromatography
partially
partially by vacuole membrane-enriched fraction preparation
-
Q Sepharose column chromatography and Sephadex S-200 gel filtration
recombinand His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli BL21(DE3) by anion exchange chromatography and gel filtration
-
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration
recombinant enzyme from Escherichia coli strain BL21
-
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, removal of the tag by thrombin
recombinant GST-tagged isozymes from Escherichia coli by glutathione affinity chromatography, elution with ethylene glcol and Mg2+
recombinant His-tagged enzyme by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography to near homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-Gami B (DE3)pLysS by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
recombinant His-tagged liver enzyme from Escherichia coli
DQ978330
recombinant His-tagged selenomethionine-enzyme from Escherichia coli strain B834(DE3) by metal affinity and anion exchange chromatography, and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, followed by gel filtration and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and nickel affinity chromatography
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, tag cleavage, and again gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, anion exchange chromatography, and gel filtration
-
recombinant MBP-His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by amylose affinity and nickel affinity chromatography, followed by TEV protease cleavage of both tags, dialysis, anion exchange chromatography, and ultrafiltration
recombinant MBP-tagged S1, S2, S3, S9-RNase and His6-tagged A14 from Escherichia coli and pulldown assay
XM_008360526
recombinant N-terminally His6-tagged enzyme 322fold from Haloferax volcanii strain H26-pJAM2920 to homogeneity by a nickel affinity chromatography, tag cleavage by thrombin, and gel filtration, followed by ultrafiltration
recombinant protein
recombinant soluble wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography
Superdex 200 gel filtration
Talon metal affinity chromatography
-
Talon metal affinity resin column chromatography and Superdex 200 gel filtration