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3.6.1.1: inorganic diphosphatase

This is an abbreviated version!
For detailed information about inorganic diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.1

Reaction

diphosphate
+
H2O
= 2 phosphate

Synonyms

acid PPase, AT727_13205, AtPPA1, AtPPsPase1, BT2127, CBS-PPase, CBS-PPase1, chloroplast inorganic pyrophosphatase 1, class A type PPA, cobalt-dependent inorganic pyrophosphatase, CPE2055, cytosolic PPase, D1C enzyme, dhPPase, E-PPase, EcPpA, EcPPiase, ehPPase, family I inorganic pyrophosphatase, family I PPase, family I pyrophosphatase, family II inorganic pyrophosphatase, family II PPase, H+-PPase, H+-pyrophosphatase, H+-translocating/vacuolar inorganic pyrophosphatase, Hpp1 protein, HVO_0729, HvPPA, hyperthermophilic inorganic pyrophosphatase, inorganic diphosphatase, inorganic PPase, inorganic pyrophosphatase, inorganic pyrophosphatase 1, inorganic pyrophosphatase-like protein, inorganic pyrophosphorylase, IPP, IPP1, Ipp1p, IPPase, LHPPase, M-PPase, manganese-dependent inorganic pyrophosphatase, MazG, MdPPa, membrane-integral inorganic pyrophosphatase, membrane-integral pyrophosphatase, miPPase, mitochondrial pyrophosphatase, Mn2+-bound canonical Family II PPase, More, Mtb PPiase, Mthe_0236, MtPPA1, MtPPiase, nucleotide-regulated pyrophosphatase, OVP1, p26.1a, p26.1b, PfPPase, PH1907, Pho-PPase, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, phosphoprotein p26.1, PPA, PPA1, PpaC, PPase, PPase 1, PPase 2, ppase-1, PPase-2, PPase1, PPase2, PPase4, PPias, PPIase, proton-translocating inorganic pyrophosphatase, PYP-1, pyrophosphatase, pyrophosphatase, inorganic, Pyrophosphate phospho-hydrolase, pyrophosphate phospho-hydrolase 1, Pyrophosphate phosphohydrolase, Pyrophosphate-energized inorganic pyrophosphatase, RmPPase, Rv3628, siPPiase, soluble inorganic pyrophosphatase, soluble inorganic pyrophosphatase 2, soluble pyrophosphatase, soluble-type yeast PPase, sPPAse, ST2226, Tc-sPPase, TC004566, TcasGA2_TC004566, TgPPase, ThPP1, Tm-PPase, TM0913, V-PPase, vacuolar H(+)-PPase, vacuolar H(+)-pyrophosphatase, vacuolar H+-pyrophosphatase, vacuolar H+-translocating inorganic pyrophosphatase, vacuolar H+-translocating pyrophosphatase, vacuolar pyrophosphatase, VHP, VP1, VSP, VVPP1

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.1 inorganic diphosphatase

Molecular Weight

Molecular Weight on EC 3.6.1.1 - inorganic diphosphatase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
103000
-
SDS-PAGE
105000
gel filtration
110000
114000
native PAGE
120000
121000
recombinant His-tagged enzyme, gel filtration
122000
-
ultracentrifuge method
128000
-
SDS-PAGE
132000
gel filtration
138000
gel filtration
17000
-
4 * 17000, SDS-PAGE, 4 * 19365, calculated
18600
4 * 18600, calculated from amino acid sequence
19100
-
6 * 19100, SDS-PAGE
19272
-
6 * 19272, amino acid sequence calculation, structure-based phylogenetic tree of diphosphatases, overview
19365
19435
-
x * 19435, calculated
20675
6 * 20675, recombinant His-tagged enzyme, mass spectrometry
20833
x * 24500, recombinant enzyme, SDS-PAGE, x * 20833, amino acid sequence calculation
20850
x * 20850, calculated from amino acid sequence
21000
21740
2 * 21740, calculated from amino acid sequence
22000
24000
240000
-
native PAGE
24350
1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry
24400
-
x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE
24484
x * 24484, isozyme PPa1, DNA sequence calculation, x * 24576, isozyme PPa4, DNA sequence calculation
24500
x * 24500, recombinant enzyme, SDS-PAGE, x * 20833, amino acid sequence calculation
24576
x * 24484, isozyme PPa1, DNA sequence calculation, x * 24576, isozyme PPa4, DNA sequence calculation
25000
26000
1 * 26000, SDS-PAGE
26500
-
x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE
27000
1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+
28000
Aranda Christine
-
gel filtration
28000 - 32000
gel filtration and native PAGE
28900
calculated from predicted amino acid sequence
29728
mass spectrometry
29850
1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry
30000
32000
32042
-
2 * 32042, amino acid sequence
32500
-
4 * 32500, SDS-PAGE
33000
33000 - 37000
gel filtration and native PAGE
33407
2 * 33407, mass spectrometry
33494
4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation
33500
4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation
34000
35000
36000
37000
-
x * 37000, SDS-PAGE
38000
-
gel filtration
41000
-
amino acid sequence
42000
43000
-
ultracentrifuge method
45000
-
x * 45000, His-tagged enzyme, SDS-PAGE
47900
-
x * 47900, calculated from amino acid sequence
50000
55000
-
gel filtration, SDS-PAGE, amino acid sequence
56000
-
ultracentrifuge method
57000
1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+
60000
60800
-
gel filtration
61000
62000
63000 - 70000
-
gel filtration
63000 - 71000
-
sedimentation equilibrium
64000
recombinant mutant I260V, native PAGE, in presence of Mn2+
64080
65000 - 70000
-
gel filtration
65500
-
gel filtration
66000
-
gel filtration
67000
70000
71000
-
gel filtration
78000
recombinant wild-type enzyme, native PAGE, in presence of Mn2+
80000
81000
-
-
additional information