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3.5.99.8: 5-nitroanthranilic acid aminohydrolase

This is an abbreviated version!
For detailed information about 5-nitroanthranilic acid aminohydrolase, go to the full flat file.

Reaction

5-nitroanthranilate
+
H2O
=
5-nitrosalicylate
+
NH3

Synonyms

5NAA aminohydrolase, 5NAA deaminase, 5NAA-A, 5NAA-aminohydrolase, NAAA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.99 In other compounds
                3.5.99.8 5-nitroanthranilic acid aminohydrolase

Cloned

Cloned on EC 3.5.99.8 - 5-nitroanthranilic acid aminohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene naaA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2/pJS803 and in strain EPI300(pJS800)
gene naaA, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta 2(DE3)