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3.5.99.10: 2-iminobutanoate/2-iminopropanoate deaminase

This is an abbreviated version!
For detailed information about 2-iminobutanoate/2-iminopropanoate deaminase, go to the full flat file.

Word Map on EC 3.5.99.10

Reaction

2-iminobutanoate
+
H2O
=
2-oxobutanoate
+
NH3

Synonyms

ACIAD3089, AtRidA, enamine/imine deaminase, endoribonuclease L-PSP family protein, imine deaminase, PA0814, PA5083, PFL_1385, PSPTO_0102, PSPTO_3006, reactive intermediate deaminase, reactive intermediate deaminase A, Rid1, Rid2, Rid3, ridA, ST0811, yjgF, YjgF_endoribonc domain-containing protein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.99 In other compounds
                3.5.99.10 2-iminobutanoate/2-iminopropanoate deaminase

Crystallization

Crystallization on EC 3.5.99.10 - 2-iminobutanoate/2-iminopropanoate deaminase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme RidA in the apo form, as well as RidA complexed with pyruvate, sitting drop vapor diffusion method, crystallization from reservoir solution containing 18% PEG 3350, 0.1 M MES, pH 6.0, 25°C, 10 days, soaking of apo-crystals in 20 mM pyruvate for 1 hour, X-ray diffraction structure determination and analysis at 2.3 A resolution
sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate