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3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

5,10-CH+-H4PteGlu cyclohydrolase, 5,10-CH+-THF cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyl-H4folate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, CHF4CH, Citrovorum factor cyclodehydrase, cyclohydrolase, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH 1, DHCH 2, DHCH1, FchA, FolD, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), methenyl-H4F cyclohydrolase, methenyl-tetrahydrofolate cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, methyleneH4folate cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, MIS1, More, mosquito cyclohydrolase, MTHFD1, MTHFD2, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Engineering

Engineering on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C58Y
kcat/Km for 5,10-methenyltetrahydrofolate is 13.2fold lower than wild-type value
D121A
kcat/Km for 5,10-methenyltetrahydrofolate is 2348fold lower than wild-type value
G122D
kcat/Km for 5,10-methenyltetrahydrofolate is 283fold lower than wild-type value
K54S
very low activity with 5,10-methenyltetrahydrofolate
Q98K
very low activity with 5,10-methenyltetrahydrofolate
R191E
kcat/Km for 5,10-methenyltetrahydrofolate is 1.3fold higher than wild-type value
Y50S
very low activity with 5,10-methenyltetrahydrofolate
D121A
-
kcat/Km for 5,10-methenyltetrahydrofolate is 2348fold lower than wild-type value
-
G122D
-
kcat/Km for 5,10-methenyltetrahydrofolate is 283fold lower than wild-type value
-
K54S
-
very low activity with 5,10-methenyltetrahydrofolate
-
R191E
-
kcat/Km for 5,10-methenyltetrahydrofolate is 1.3fold higher than wild-type value
-
Y50S
-
very low activity with 5,10-methenyltetrahydrofolate
-
D125A
site-directed mutagenesis, inactive mutant
D125C
site-directed mutagenesis, inactive mutant
D125E
site-directed mutagenesis, 95% reduced activity in the forward reaction compared to the wild-type enzyme
D125M
site-directed mutagenesis, inactive mutant
D125N
site-directed mutagenesis, inactive mutant
D125Q
site-directed mutagenesis, inactive mutant
K56Q/Q100K
Q100K
site-directed mutagenesis, inactive mutant
Q100M
site-directed mutagenesis, inactive mutant
Q100N
site-directed mutagenesis, inactive mutant
R173A
-
site-directed mutagenesis, at least 500fold increased Km for NADP+ compared to the wild-type, 163fold increased activity in the forward and reduced activity in the reverse reaction of the cyclohydrolase, unaltered channeling efficiency
R173E
-
site-directed mutagenesis, no dehydrogenase forward reaction activity, no forward channeling
R173K
-
site-directed mutagenesis, at least 500fold increased Km for NADP+ compared to the wild-type, 46fold increased activity in the forward and reduced activity in the reverse reaction of the cyclohydrolase, unaltered channeling efficiency
R250A
site-directed mutagenesis, 29.7% reduced activity in forward and reduced activity in the reverse reaction compared to the wild-type enzyme
R653Q
S197A
-
site-directed mutagenesis, 20fold increased Km for NADP+ compared to the wild-type, reduced activity in the reverse reaction of the cyclohydrolase, no stimulation by 2',5'-ADP, unaltered channeling efficiency
S197D
-
site-directed mutagenesis, 8.5fold increased activity in the forward reaction of the cyclohydrolase, unaltered channeling efficiency
S197R
-
site-directed mutagenesis, 10fold increased Km for methenyltetrahydrofolate, reduced activity in the forward and reverse reaction of the cyclohydrolase, unaltered channeling efficiency
S197T
-
site-directed mutagenesis, slightly increased activity in the forward reaction of the cyclohydrolase, unaltered channeling efficiency
Y240A
site-directed mutagenesis, 52,5% reduced activity in forward and reduced activity in the reverse reaction compared to the wild-type enzyme
additional information