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3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

5,10-CH+-H4PteGlu cyclohydrolase, 5,10-CH+-THF cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyl-H4folate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, CHF4CH, Citrovorum factor cyclodehydrase, cyclohydrolase, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH 1, DHCH 2, DHCH1, FchA, FolD, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), methenyl-H4F cyclohydrolase, methenyl-tetrahydrofolate cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, methyleneH4folate cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, MIS1, More, mosquito cyclohydrolase, MTHFD1, MTHFD2, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Cloned

Cloned on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6His-tagged enzyme is expressed in Escherichia coli
amplified genomic library transformed into yest YA3-1, yeast-DNA transformed into Escherichia coli K38 pBKe, gene folD encoding PPDC expressed
-
bifunctional dehydrogenase/cyclohydrolase portion of the cytoplasmic trifunctional enzyme cloned and heterologous expressed in the yeast strain YA3-1
-
cDNA encoding bifunctional enzyme cloned and expressed in Escherichia coli K38
-
cDNA encoding trifunctional enzyme used to complete the purine auxotrophic yeast strain YA3-1
-
deletion of the MIS1 gene has little effect
DNA fragment containing the bifunctional enzyme from Escherichia coli BE cloned and transformed into Escherichia coli BL21 for protein expression
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain BL21 (DE3)
-
expression in Escherichia coli BL21
expression in Escherichia coli BL21(DE3)
expression of the His-tagged enzyme in Escherichia coli
expression of the N-terminally 6-His-tagged MTHFDC in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant enzymes in Escherichia coli BL21 as C-terminally His-tagged enzymes
expression of wild-type and mutant enzymes in Escherichia coli JM109 as C-terminally His-tagged enzymes
-
gene folD for bifunctional dehydrogenase/cyclohydrolase cloned and sequenced
-
heterologously overproduced in Escherichia coli with a C-terminal His6-tag
hexa-His tagged DHCH1 is expressed in Escherichia coli, production of a genetic knock-out
-
null mutation is embryonic lethal at about 12 days gestation, knockout of enzyme establishes the important role of formate production during embryogenesis
-
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein