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3.5.4.5: cytidine deaminase

This is an abbreviated version!
For detailed information about cytidine deaminase, go to the full flat file.

Word Map on EC 3.5.4.5

Reaction

2'-deoxycytidine
+
H2O
=
2'-deoxyuridine
 +
NH3

Synonyms

activation-induced cytidine deaminase, AICDA, canine hepatic cyd deaminase, CDA, CDABcald, CDABpsy, CDABsub, CDase, CDD, CR deaminase, cyd deaminase, Cytidine aminohydrolase, cytidine deaminase, cytidine/2'-deoxycytidine aminohydrolase, cytosine nucleoside deaminase, DCD, dCDA, deoxycytidine deaminase, EC 3.5.4.14, mammalian deminase, T-CDA, yeast cytidine deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase

Storage Stability

Storage Stability on EC 3.5.4.5 - cytidine deaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity
-
-20°C, 0.005 M dithiothreitol, enzymatic activity is stable for many weeks
-
-20°C, 20 mM Tris-HCl, pH 7.5, 1 mM DDT, 1 mM EDTA, 20% glycerol, enzyme retains full activity for at least 1 month, 80% of activity lost in absence of glycerol
-
-20°C, 20% glycerol appears to be necessary to prevent inactivation from occuring after freezing and thawing
-
-20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity
-
-20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity
-
-20°C, 50% glycerol, very stable, litle loss of enzymatic activity after 1 year
-
-20°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
-
-20°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
-
-20°C, preparations kept 4 months retains original activity
-
-20°C, shows no appreciable loss of activity within 1 month
-
4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks
-
4°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
-
4°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
-
4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle
-
4°C, reasonably stable, retains 55% of its activity after 3 weeks
-
4°C, stable for several days, may be kept for months without significant loss of activity if frozen
-
4°C, stable for some hours at pH 5.5-9.5
-
4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature
-
4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks
-