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3.5.4.5: cytidine deaminase

This is an abbreviated version!
For detailed information about cytidine deaminase, go to the full flat file.

Word Map on EC 3.5.4.5

Reaction

2'-deoxycytidine
+
H2O
=
2'-deoxyuridine
 +
NH3

Synonyms

activation-induced cytidine deaminase, AICDA, canine hepatic cyd deaminase, CDA, CDABcald, CDABpsy, CDABsub, CDase, CDD, CR deaminase, cyd deaminase, Cytidine aminohydrolase, cytidine deaminase, cytidine/2'-deoxycytidine aminohydrolase, cytosine nucleoside deaminase, DCD, dCDA, deoxycytidine deaminase, EC 3.5.4.14, mammalian deminase, T-CDA, yeast cytidine deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase

Crystallization

Crystallization on EC 3.5.4.5 - cytidine deaminase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of tetrameric cytidine deaminase at 2.0 A resolution, hanging drop vapour-diffusion method
hanging drop vapour diffusion method. R56A and R56Q crystallize in the same space group as the wild-type enzyme with two subunits in the asymmetric unit, whereas C53H/R56Q can not crystallize in this crystal form but is crystallized in another space group, P3(2)21, with a full tetramer in the asymmetric unit
comparison of theoretically predicited model and experimentally elucidated structure
-
in complex with inhibitor diazepinone riboside. Inhibitor is able to establish a canonical pi/pi interaction with key active site residue F137
-
to 2.07 A resolution. Two 1,4-dioxane moleules are present at the active site
in complex with tetrahydrouridine, 3-deazauridine, or cytidine. Two alternate conformations of R68 influence zinc-product interaction
purified recombinant CDA complexed with either tetrahydrouridine, 3-deazauridine, or cytidine, hanging drop vapor diffusion technique, 25°C, protein solution, containing 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 1 mM DTT, and 5 mM ligand, is mixed with crystallization solution containing 2.0 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, crystals appear after a few hours or several days, respectively, X-ray diffraction structure determination and analysis at 1.48-2.36 A resolutions, molecular replacement
hanging drop vapor diffusion method
purified CDA in complex with uridine and deoxyuridine, hanging drop vapor diffusion method, 0.002 ml of 12 mg/ml protein in 20 mM Tris-HCl pH 7.5 is mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5 and 4.3 M sodium chloride, ligands uridine and deoxyuridine are added by soaking method, X-ray diffractiuon structure determination and analysis at 2.4 and 1.9 A resolution,molecular dynamics simulation, structure modeling
structures of apomutants E47D and E47Q