3.5.4.42: N-isopropylammelide isopropylaminohydrolase

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For detailed information about N-isopropylammelide isopropylaminohydrolase, go to the full flat file.

Reaction

Synonyms

amidase, N-isopropylammelide, AtzC, EC 3.5.99.4, IAIA, N-isopropylammelide amidase, N-isopropylammelide isopropylamidohydrolase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.4 In cyclic amidines

3.5.4.42 N-isopropylammelide isopropylaminohydrolase

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Results	in table
1	Activating Compound
537	AA Sequence
15	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Crystallization (Commentary)
2	Expression
3	General Information
10	Inhibitors
14	kcat/KM [mM/s]
3	Ki Value [mM]
22	KM Value [mM]
4	Metals/Ions
4	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
50	Organism
6	Pathway
1	PDB ID
16	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
38	Reference
1	Renatured (Commentary)
2	Source Tissue
1	Specific Activity [micromol/min/mg]
53	Substrates and Products (Substrate)
2	Subunits
56	Synonyms
1	Systematic Name
3	Temperature Stability [°C]
14	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 3.5.4.42 - N-isopropylammelide isopropylaminohydrolase

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1,10-phenanthroline

Pseudomonas sp.

5 mM, 30% residual activity, Mn2+, Co2+ or Ni2+ restores

651707

2-amino-4-hydroxy-1,3,5-s-triazine

Pseudomonas sp.

i.e, 5-azacytosine, competitive

651707

8-Hydroxyquinoline-5-sulfonic acid

Pseudomonas sp.

5 mM, 30% residual activity, Mn2+, Ni2+ or Co2+ restores

ammeline

competitive

EDTA

5 mM, about 10% loss of activity

651707

N-ethylammeline

Pseudomonas sp.

651707

N-hydroxyethylammeline

competitve

N-isopropylammeline

nitrate

nitrate completely inhibits atrazine degradation activity in strain KU001

720208

additional information

Arthrobacter sp.

ammonium and urea have no effect on atrazine degradation activity in strain KU001

720208