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3.5.4.4: adenosine deaminase

This is an abbreviated version!
For detailed information about adenosine deaminase, go to the full flat file.

Word Map on EC 3.5.4.4

Reaction

adenosine
+
H2O
=
Inosine
 +
NH3

Synonyms

AD, ADA, ADA1, ADA2, ADAI, ADAII, ADAIII, Adenosine aminohydrolase, adenosine deaminase, adenosine deaminase 1, adenosine deaminase 2, ciADA, HadA, LADA, MadA, MJ1541, PvADA, SADA, SCO4901, TadA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.4 adenosine deaminase

Cloned

Cloned on EC 3.5.4.4 - adenosine deaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme expression in an enzyme-deficient Escherichia coli strain Sphi3834
-
expressed in Escherichia coli BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli S Phi 3834
-
expression of His-tagged enzyme in Escherichia coli strain BL21
-
isozyme ADA2 is expressed in S2 Drosophila cells
overexpression in Escherichia coli BL21 (DE3)
the activity of human adenosine deaminase (hADA) expressed in transgenic seeds of three different plant species: pea (Pisum sativum L.), Nicotiana benthamiana L. and tarwi (Lupinus mutabilis Sweet) is compared. All three species are transformed with the same expression vector containing the hADA gene driven by the seed-specific promoter LegA2 with an apoplast targeting pinII signal peptide. During the study, several independent transgenic lines are generated and screened from each plant species and only lines with a single copy of the gene of interest are used for hADA expression analysis. A stable transgenic canola line expressing the ADA protein, under the control of 35S constitutive promoter is used as both as a positive control and for comparative study with the seed specific promoter. The highest activity of the hADA enzyme (U/g seed) is reported in tarwi (4.26 U/g) followed by pea (3.23 U/g) and Nicotiana benthamiana (1.69 U/g)
wild type enzyme is expressed in Escherichia coli strain TOP10, mutant enzymes are expressed in Escherichia coli strain BL21-codon plus (DE3)-RIPL
-