3.5.4.34: tRNAAla(adenine37) deaminase
This is an abbreviated version!
For detailed information about tRNAAla(adenine37) deaminase, go to the full flat file.
Word Map on EC 3.5.4.34
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3.5.4.34
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deaminases
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inosine
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pre-mrnas
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deamination
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trna-specific
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adats
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melanogaster
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anticodon
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drosophila
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sequence-related
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dsrnas
- 3.5.4.34
- deaminases
- inosine
- pre-mrnas
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deamination
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trna-specific
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adats
- melanogaster
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anticodon
- drosophila
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sequence-related
- dsrnas
Reaction
Synonyms
ADAT1, hADAT1, Tad1p/scADAT1
ECTree
3.5.4.34 tRNAAla(adenine37) deaminaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.5.4.34 - tRNAAla(adenine37) deaminase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
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?
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
the enzyme is specific for adenosine 37 of insect alanine tRNA, it has no activity on dsRNA substrates. Drosophila ADAT1 binds and deaminates tRNAAla from Bombyx mori 100fold more efficiently than the tRNAAla from Saccharomyces cerevisiae
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?
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
specifically deaminates adenosine 37 to inosine in eukaryotic tRNAAla. This unique modification in tRNAAla is conserved from yeast to man
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?
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
in vitro, the recombinantly expressed and purified hADAT1 protein efficiently and specifically deaminates A37 in the anticodon loop of tRNAAla from higher eukaryotes and with lower efficiency from lower eukaryotes. It does not modify adenosines residing in double-stranded RNA or in pre-mRNAs that serve as substrates for ADAR1 or ADAR2. The anticodon stemloop of tRNAAla alone is not a functional substrate for hADAT1
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?
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
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?
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
pre-mRNA and extended dsRNA are not used as substrates by Tad1p in vitro. Tad1p requires both the local conformation of the anticodon loop and a correct folding of the L-shaped tRNAAla substrate to recognize and deaminate A37
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?
