3.5.4.32: 8-oxoguanine deaminase
This is an abbreviated version!
For detailed information about 8-oxoguanine deaminase, go to the full flat file.
Reaction
Synonyms
8-OGD, 8-oxoGD, Pa0142
ECTree
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Substrates Products on EC 3.5.4.32 - 8-oxoguanine deaminase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
8-oxoguanine + H2O
8-oxoxanthine + NH3
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8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions
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-
?
8-oxoguanine + H2O
urate + NH3
8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions
-
-
?
8-oxoguanine + H2O
urate + NH3
kcat/Km value of 8-oxoguanine is an order of magnitude larger than that for the next best compound tested
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?
additional information
?
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the rate-limiting step corresponds to the nucleophilic attack from the zinc-coordinating hydroxide group to free 8-oxoguanine. Residues Trp115, Trp123 and Leu119 connect to O8 of 8-oxoguanine with hydrogen bonds. Both Glu218 and a water molecule can be used as proton shuttles, and a water molecule plays a major role in proton transfer to the substrate
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?
additional information
?
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the rate-limiting step corresponds to the nucleophilic attack from the zinc-coordinating hydroxide group to free 8-oxoguanine. Residues Trp115, Trp123 and Leu119 connect to O8 of 8-oxoguanine with hydrogen bonds. Both Glu218 and a water molecule can be used as proton shuttles, and a water molecule plays a major role in proton transfer to the substrate
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?
