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3.5.4.3: guanine deaminase

This is an abbreviated version!
For detailed information about guanine deaminase, go to the full flat file.

Word Map on EC 3.5.4.3

Reaction

guanine
+
H2O
=
xanthine
 +
NH3

Synonyms

Agdap, cypin, cytosolic PSD-95 interactor, E. coli guanine deaminase, GAH, GDA, GDEase, GUA, guaD, guanase, guanine aminase, Guanine aminohydrolase, guanine deaminase, GUD1, human guanine deaminase, KLLAOD9492, More, NE0047, p51-nedasin, YgfP

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.3 guanine deaminase

Storage Stability

Storage Stability on EC 3.5.4.3 - guanine deaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, isoenzyme A loses about 60% of activity on thawing after 7 days, isoenzyme B is unaltered in activity
-
-20°C stable for 2 weeks with only a few percent loss
-
-20°C, 28 days, 123% activity
-
-20°C, with 20% (v/v) glycine, 28 days, 65% residual activity
-
0-4°C, activity of isoenzyme A completely lost in 16 days, isoenzyme B loses 30% activity
-
0°C, 10 mM phosphate buffer, pH 7.0, 0.1 mM dithiothreitol, can be stored for more than 2 weeks without appreciable loss of activity
-
0°C, retains its activity unaltered during storage for a month
-
22°C, 28 days, 34% residual activity
-
4°C stable for 2 weeks with only a few percent loss
-
4°C, 0.1 M phosphate buffer, 2 mM 2-mercaptoethanol, pH 7.5, stable for at least 3 months
-
4°C, 28 days, 44% residual activity
-
4°C, 50 mM Tris, 100 mM NaCl, 10% glycerol and pH 7.0, stable for a month
-
purified enzyme stabilized by storage in 30-40% glycerol
-