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3.5.4.3: guanine deaminase

This is an abbreviated version!
For detailed information about guanine deaminase, go to the full flat file.

Word Map on EC 3.5.4.3

Reaction

guanine
+
H2O
=
xanthine
 +
NH3

Synonyms

Agdap, cypin, cytosolic PSD-95 interactor, E. coli guanine deaminase, GAH, GDA, GDEase, GUA, guaD, guanase, guanine aminase, Guanine aminohydrolase, guanine deaminase, GUD1, human guanine deaminase, KLLAOD9492, More, NE0047, p51-nedasin, YgfP

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.3 guanine deaminase

Specific Activity

Specific Activity on EC 3.5.4.3 - guanine deaminase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00011
-
crude extract, at 50°C, using 7,8-dihydropterin as substrate, pH not specified in the publication
0.1
-
crude extract, at pH 6.0 and 37°C
0.6435
-
after 5850fold purification, at 50°C, using 7,8-dihydropterin as substrate, pH not specified in the publication
1.14
-
purified liver enzyme
1.36
-
kidney enzyme
11.2
-
intestine enzyme, purification at 0-4°C
1140
-
crude cell extract, pH 7.5, temperature not specified in the publication
13.2
-
brain enzyme
13.7
-
purified brain enzyme
16.8
-
brain enzyme, purification at room temperature
17.2
-
liver enzyme, purification at room temperature
19.3
-
liver enzyme, purification at 0-4°C
2.42
-
brain enzyme
21.5
-
-
21.7
-
-
22.1
-
-
22.4
-
brain enzyme, purification at 0-4°C
23.5
-
lung enzyme
25.2
-
liver enzyme
25.4
-
intestine enzyme, purification at room temperature
33.6
-
isoenzyme B
4.7
-
after 51.5fold purification, at pH 6.0 and 37°C
5.1
-
liver enzyme
530
-
crude cell extract, pH 7.5, temperature not specified in the publication
8.61
-
-
9.53
recombinant enzyme
9.9
-
liver enzyme, isoenzyme A
additional information
-
colorimetric and HPLC assay method using guanosine as a prosubstrate. Method is suitable for routine assays for measuring plasma enzyme over a wide range of activites