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3.5.4.3: guanine deaminase

This is an abbreviated version!
For detailed information about guanine deaminase, go to the full flat file.

Word Map on EC 3.5.4.3

Reaction

guanine
+
H2O
=
xanthine
+
NH3

Synonyms

Agdap, cypin, cytosolic PSD-95 interactor, E. coli guanine deaminase, GAH, GDA, GDEase, GUA, guaD, guanase, guanine aminase, Guanine aminohydrolase, guanine deaminase, GUD1, human guanine deaminase, KLLAOD9492, More, NE0047, p51-nedasin, YgfP

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.3 guanine deaminase

Engineering

Engineering on EC 3.5.4.3 - guanine deaminase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A386S
4.8fold decrease in kcat/Km value
D327L
no residual activity
E240D
no residual activity
F210A
20fold decrease in kcat/Km value
H237R
no residual activity
L305A
no residual activity
Q87N
no residual activity
W101H
8.9fold decrease in kcat/Km value
E143X
the mutation results in a complete loss of activity
E79X
the mutation results in a complete loss of activity
D330A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H240A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H279A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H71A
-
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme
H82A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H84A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
I83A
-
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme
additional information