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3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydromethanopterin cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.27

Reaction

5,10-methenyl-5,6,7,8-tetrahydromethanopterin
+
H2O
=
5-formyl-5,6,7,8-tetrahydromethanopterin

Synonyms

5,10-methenyltetrahydromethanopterin cyclohydrolase, CBH37751, cyclohydrolase, hydrolase, methenyltetrahydromethanopterin cyclo-, Mch, methenyl-H4MPT cyclohydrolase, methenyl-H4MPT+ cyclohydrolase, methenyltetrahydromethanopterin cyclohydrolase, N5,N10-methenyltetrahydromethanopterin cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.27 methenyltetrahydromethanopterin cyclohydrolase

Storage Stability

Storage Stability on EC 3.5.4.27 - methenyltetrahydromethanopterin cyclohydrolase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified native enzyme, 1.32 M KCl, 50 mM Tris-HCl, pH 7.0, under N2 atmosphere, several months without significaant loss of activity
-
-20°C, stable for several weeks
-
-20°C, under N2, 50 mM Tris/HCl buffer, pH 7, containing 1.3 M KCl, no loss of activity for several months
-
-20°C, under N2, in 50 mM Tris/HCl, pH 7, containing 1.3 M KCl, stable for several months
-
0°C, both under anaeobic and aerobic conditions, the enzyme also loses very little activity within 24 h
-
0°C, both under anaerobic and anaerobic conditions, stable for 24 h
-