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3.5.4.23: blasticidin-S deaminase

This is an abbreviated version!
For detailed information about blasticidin-S deaminase, go to the full flat file.

Word Map on EC 3.5.4.23

Reaction

blasticidin S
+
H2O
=
deaminohydroxyblasticidin S
 +
NH3

Synonyms

Bc S deaminase, blasticidin S deaminase, BS deaminase, BSD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.23 blasticidin-S deaminase

Cloned

Cloned on EC 3.5.4.23 - blasticidin-S deaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bsd overexpressed in Escherichia coli BL 21
-
bsr gene employed as marker gene for Escherichia coli, Bacillus subtilis, Nicotiana tabacum, Rhizopus niveus and cultured mammalian cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)/pLysE cells
-
expression of deletion mutants as soluble enzymes in Escherichia coli
generating of bicistronic vectors express both blastidicin S deaminase gene and a fusion gene consisting of a sarcomeric protein, selection with blasticidin S results in a relatively pure population of ES cell-derived cardiomycetes
-
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3), Cys91 mutants are expressed in inclusion bodies
-
pBSR8 with structural gene bsr cloned and expressed in both Escherichia coli JM109 and Bacillus subtilis 168
-
PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD
-
plasmid pAUR123-BSD, transformation of industrial yeast Saccharomyces cerevisiae X-2180AB
-
pTPBS1, Arabidopsis thaliana and Nicotiana tabacum transformed to blasicidin S resistance
-
recombinant enzyme overexpressed in Escherichia coli
-
selectable marker for mammalian cells
-