3.5.4.23: blasticidin-S deaminase

This is an abbreviated version!
For detailed information about blasticidin-S deaminase, go to the full flat file.

Word Map on EC 3.5.4.23

3.5.4.23

license

bipolar

freely

hyperthermia

user

python

open-source

phantom

linux

disinfestation

github

autometallography

hypomanic

deep-seated

strut

molecular biology

biotechnology

Reaction

Synonyms

Bc S deaminase, blasticidin S deaminase, BS deaminase, BSD

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.4 In cyclic amidines

3.5.4.23 blasticidin-S deaminase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
6	Activating Compound
87	AA Sequence
4	Application
1	CAS Registry Number
19	Cloned(Commentary)
3	Crystallization (Commentary)
2	General Stability
25	Inhibitors
8	KM Value [mM]
3	Metals/Ions
14	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
40	Organism
26	PDB ID
3	pH Optimum
2	pH Range
3	pH Stability
12	Protein Variants
9	Purification (Commentary)
3	Reaction
3	Reaction Type
37	Reference
2	Renatured (Commentary)
1	Specific Activity [micromol/min/mg]
2	Storage Stability
77	Substrates and Products (Substrate)
9	Subunits
15	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
6	Turnover Number [1/s]

Application

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Application on EC 3.5.4.23 - blasticidin-S deaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Application Search

biotechnology

Phaeodactylum tricornutum

the enzyme is a selection marker for genetic transformation of the diatom Phaeodactylum tricornutum. An easy handle system of antibiotic selection with blasticidin-S and the resistance gene bsr in Phaeodactylum tricornutum is reported. Blasticidin-S works by blocking protein translation and therefore it is not expected to affect the genomic DNA of transformed cells. Especially for reverse genetics studies via genome editing, when phenotypes should be determined based on cell-linages with as less off-target genetic changes as possible, utilization of this antibiotic is a major improvement. Additionally, blasticidin-S selection can be used in combination with nourseothricin- and potentially also with Zeocin-resistant cells. This extension of the genetic toolbox can be useful for further molecular characterization or biotechnological application of the model diatom Phaeodactylum tricornutum and possibly for other species of diatoms

757914

molecular biology

3 entries