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3.5.4.16: GTP cyclohydrolase I

This is an abbreviated version!
For detailed information about GTP cyclohydrolase I, go to the full flat file.

Word Map on EC 3.5.4.16

Reaction

GTP
+
H2O
=
formate
 +
7,8-dihydroneopterin 3'-triphosphate

Synonyms

dihydroneopterin triphosphate synthase, FelE, folE, GCH, GCH-1, GCH1, GCHI, GCYH-IA, GCYH-IB, GTP 8-formylhydrolase, GTP CHase I, GTP cyclohydrolase, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP cyclohydrolase IA, GTP cyclohydrolase IB, GTP cyclohydrolase-1, GTP-CH, GTP-CH-I, GTP-CH1, GTP-cyclohydrolase 1, GTP-cyclohydrolase I, GTPCH, GTPCH I, GTPCH-1, GTPCH1, GTPCHI, guanosine 5'-triphosphate-cyclohydrolase I, guanosine triphosphate 8-deformylase, guanosine triphosphate cyclohydrolase, guanosine triphosphate cyclohydrolase 1, guanosine triphosphate cyclohydrolase I, guanosine-5'-triphosphate cyclohydrolase 1, hydrolase, guanosine triphosphate cyclo-, MptA, Punch, Punch protein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.16 GTP cyclohydrolase I

Purification

Purification on EC 3.5.4.16 - GTP cyclohydrolase I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-300HR gel filtration
-
expression in Escherichia coli
-
expression of wild-type and mutant proteins in Escherichia coli
-
GCH1 in complexes with cellular proteins from organs liver, heart, brain, and kidney, by immunoaffinity chromatography, isolation of mitochondria from liver, heart and kidney
-
Mono Q HR column chromatography
-
Ni-NTA resin column chromatography
-
Ni2+-Sepharose column chromatography, and gel filtration
recombinant DELTA42-hGTP-CH-I from Escherichia coli M15
recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography
recombinant HAT-tagged enzyme from Escherichia coli, 6.8fold
recombinant His-tagged wild-type GTPCH maltose-binding protein fusion protein from Escherichia coli by amylose affinity chromatography and cleavage of the fusion tag by TEV protease, purification of His-tagged DELTA45-GTPCH mutant from HEK-293 cells by metal affinity chromatography
-
recombinant maltose binding protein fusion enzyme fromn Escherichia coli strain DH5alpha
-
recombinant wild-type and mutant enzymes
-
two forms
-