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3.5.3.9: allantoate deiminase

This is an abbreviated version!
For detailed information about allantoate deiminase, go to the full flat file.

Word Map on EC 3.5.3.9

Reaction

Allantoate
+
H2O
=
(S)-ureidoglycine
+
NH3
+
CO2

Synonyms

AAH, ADE2, ALAH, Allantoate amidinohydrolase, allantoate amidohydrolase, allantoate-degrading enzyme, ALLC, AtAAH

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.9 allantoate deiminase

Purification

Purification on EC 3.5.3.9 - allantoate deiminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
579fold purified ond DEAE-Sephadex column, ADE2 pool
-
gel filtration with Sephacryl S-200 and Sephadex A50 columns, and dialysis against Tris-HCl pH 7.5 at 4°C
-
native enzyme 22.4fold by ultracentrifugation and batch separation using an anion exchanger resin
-
recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography
StrepTactin Sepharose column chromatography