3.5.3.22: proclavaminate amidinohydrolase

This is an abbreviated version!
For detailed information about proclavaminate amidinohydrolase, go to the full flat file.

Reaction

Synonyms

PAH, PAH2, proclavaminate amidino hydrolase, proclavaminate amidinohydrolase, Proclavaminic acid amidino hydrolase, proclavaminic acid amidino hydrolaseproclavaminic acid amidino hydrolase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.3 In linear amidines

                3.5.3.22 proclavaminate amidinohydrolase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
144	AA Sequence
2	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Crystallization (Commentary)
1	Expression
1	General Information
2	KM Value [mM]
5	Metals/Ions
5	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
4	Pathway
1	pH Optimum
1	Protein Variants
2	Purification (Commentary)
3	Reaction
1	Reaction Type
8	Reference
13	Substrates and Products (Substrate)
4	Subunits
9	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 3.5.3.22 - proclavaminate amidinohydrolase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Streptomyces clavuligerus

-

639016, 639018

amplified from Streptomyces clavuligerus genomic DNA. Cloned in the pSET152 integration and pIBR25 expression vectors containing the strong ermE promoter. Plasmids are introduced into Streptomyces clavuligerus by protoplast transformation

Streptomyces clavuligerus

-

719152

expressed in Streptomyces clavuligerus strain SMF5704

Streptomyces clavuligerus

-

688326

expression in Streptomyces venezuelae

Streptomyces clavuligerus

B5GLC8, P0DJQ3

742159

Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028

Streptomyces clavuligerus

B5GLC8, P0DJQ3

742159