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3.5.3.18: dimethylargininase

This is an abbreviated version!
For detailed information about dimethylargininase, go to the full flat file.

Word Map on EC 3.5.3.18

Reaction

Nomega,Nomega'-dimethyl-L-arginine
+
H2O
=
Dimethylamine
 +
L-citrulline

Synonyms

Adv-DDAH, DDAH, DDAH-1, DDAH-2, DDAH-I, DDAH1, DDAH2, DDAHI, DDAHII, dimethlarginine dimethylaminohydrolase, dimethylaminohydrolase, Dimethylargininase, dimethylarginine dimethylamino-hydrolase, dimethylarginine dimethylaminohydrolase, dimethylarginine dimethylaminohydrolase 1, dimethylarginine dimethylaminohydrolase isoform 1, dimethylarginine dimethylaminohydrolase-2, dimethylarginine dimethylaminohydrolase1, dimethylarginine-dimethylaminohydrolase, G6a, hDDAH-1, human DDAH-1, human dimethylarginine dimethylaminohydrolase-1, Nomega,Nomega-dimethyl-L-arginine dimethylaminohydrolase-1, PA1195, PaDDAH

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.18 dimethylargininase

Engineering

Engineering on EC 3.5.3.18 - dimethylargininase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C274A
C275A
-
retains a kcat value about half of wild type protein
H173A
-
no detectable activity
C249S
D244N
-
wild type and D244N DDAH variants both form covalent adducts upon incubation with 2-hydroxymethyl-4-chloropyridine, showing mass additions that are consistent with covalent attachment of one equivalent of hydroxymethylpyridine to each enzyme. These results also indicate that Asp244 is not essential for covalent modification to occur
D66N
-
the C249S mutant and D66N mutant are both incapable of forming a covalent adduct when incubated with 2-hydroxymethyl-4-chloropyridine
E33H
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
E33Q
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
H162G
N36D
expressed in Escherichia coli at levels similar to the wild type
N36H
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
N36W
more stable homodimer than the wild type protein, expressed in Escherichia coli at levels similar to the wild type
Q43H
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
Q43R
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
R40E
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
R40E-R98H
exclusively monomeric, expressed in Escherichia coli at levels similar to the wild type
R40E/R98H
about 95% of wild type activity, stable monomer
R40W
not expressed in Escherichia coli at levels similar to the wild type
R98H
weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type
R98N
not expressed in Escherichia coli at levels similar to the wild type
S248N
-
mutant is still capable of substrate turnover and is still inactivated by 2-hydroxymethyl-4-chloropyridine with a second order inactivation rate constant that is approximately 2fold less than wild type DDAH