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3.5.3.12: agmatine deiminase

This is an abbreviated version!
For detailed information about agmatine deiminase, go to the full flat file.

Word Map on EC 3.5.3.12

Reaction

agmatine
+
H2O
=
N-Carbamoylputrescine
+
NH3

Synonyms

AgD, AgDI, agmatine amidinohydrolase, agmatine deiminase, agmatine deiminaseHpAgD, agmatine iminohydrolase, agu2A, AguA, aguA protein, AguA1, aguA2, AIH, More, PgAgD, putrescine synthase, SmAgD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.12 agmatine deiminase

Purification

Purification on EC 3.5.3.12 - agmatine deiminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2470fold
-
enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography
-
HisTrap Ni-affinity column chromatography and Superdex 200 HR gel filtration
nickel-chelated agarose gel column chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography
strain PAO4495, 21fold purified to near homogeneity, gel filtration
-
the protein is recombinantly expressed, lysed by sonication, and then purified by a combination of anion exchange, heparin affinity, and gel filtration