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8.2 - 84
(2S,5R,6R)-6-[[(2R)-2-carboxy-2-thiophen-3-ylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
1.02 - 1180
(6R,7R)-3-[(acetyloxy)methyl]-7-(formylamino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
1.6
(6R,7R)-3-[(acetyloxy)methyl]-7-(hydroxyamino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.46
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.32
(6R,7R)-3-[(acetyloxy)methyl]-7-[formyl(hydroxy)amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.121 - 0.49
(6R,7R)-3-[(acetyloxy)methyl]-7-[hydroxy(phenylacetyl)amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
0.45 - 1.5
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
1.81 - 690
(6R,7R)-3-[(acetyloxy)methyl]-8-oxo-7-[(thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
0.08 - 1.6
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
2.56
(6R,7Z)-3-[(acetyloxy)methyl]-7-(hydroxyimino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.54
(6R,7Z)-7-(hydroxyimino)-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate 5,5-dioxide
-
pH and temperature not specified in the publication
0.25
3-([3-dibenzylamino-3-oxopropanoyl]oxy)benzoic acid
pH 7.5, 25°C
0.33
3-([3-[benzyl(methyl)amino]-3-oxopropanoyl]oxy)benzoic acid
pH 7.5, 25°C
25 - 125
3-([N-(phenylacetyl)glycyl]oxy)benzoic acid
11 - 19
3-([N-[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]glycyl]oxy)benzoic acid
2.9
3-([[(2R)-3-(benzylamino)-2-methoxy-3-oxopropanoyl]oxy]methyl)benzoic acid
pH 7.5, 25°C
1.17
3-([[(2R)-3-(benzylamino)-2-methyl-3-oxopropanoyl]oxy]methyl)benzoic acid
pH 7.5, 25°C
7.4
3-([[(2S)-3-(benzylamino)-2-methoxy-3-oxopropanoyl]oxy]methyl)benzoic acid
pH 7.5, 25°C
6.6
3-([[(2S)-3-(benzylamino)-2-methyl-3-oxopropanoyl]oxy]methyl)benzoic acid
pH 7.5, 25°C
0.05
3-([[2-benzyl-3-oxo-3-(benzylamino)propanoyl]oxy]methyl)benzoic acid
pH 7.5, 25°C
10.9
3-nitrophenyl (2S)-2-hydroxy-3-phenylpropanoate
pH not specified in the publication, temperature not specified in the publication
11 - 19
3-[2-(2-aminothiazol-4-yl)2-(Z)-methoxyiminoacetylglycyl]oxybenzoic acid
6.9
3-[[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]benzoic acid
pH not specified in the publication, temperature not specified in the publication
659
6-aminopenicillanic acid
-
-
300
6-beta-iodopenicillanate
-
-
667
7-(thienyl-2-acetamido)-3-[2-(4-,N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid
-
-
50
7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid
-
-
5.9
benzyl [[(2R)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate
pH not specified in the publication, temperature not specified in the publication
3.5
benzyl [[(2S)-2-hydroxy-2-phenylacetyl]oxy]carbamate
pH not specified in the publication, temperature not specified in the publication
8.7
benzyl [[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate
pH not specified in the publication, temperature not specified in the publication
1.5
benzyl [[(2S)-2-hydroxy-4-phenylbutanoyl]oxy]carbamate
pH not specified in the publication, temperature not specified in the publication
1.5
benzyl [[(2S)-2-hydroxypropanoyl]oxy]carbamate
pH not specified in the publication, temperature not specified in the publication
0.004 - 2650
benzylpenicillin
0.004 - 1200
carbenicillin
110
cefcapene
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
83
cefdinir
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
0.21 - 229.5
cefoperazone
25
cefsulodine
30°C, pH 7.0
0.3
ceftaxidime
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
0.00833 - 160
ceftazidime
120
cephaloridin
30°C, pH 7.0
0.002 - 2530
cephaloridine
0.2 - 280
cephalosporin C
0.0023 - 7150
cephalothin
35
Penicillin V
-
in 100 mM MES (pH 6.4)
2550
phenoxymethylpenicillin
-
-
0.0005
tebipenem
pH 6.5, 22°C, recombinant enzyme
0.3
temocillin
pH 7.0, 30°C
additional information
aztreonam
8.2
(2S,5R,6R)-6-[[(2R)-2-carboxy-2-thiophen-3-ylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
beta-lactamases CTX-M-25
84
(2S,5R,6R)-6-[[(2R)-2-carboxy-2-thiophen-3-ylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
beta-lactamases CTX-M-26
1.02
(6R,7R)-3-[(acetyloxy)methyl]-7-(formylamino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
1180
(6R,7R)-3-[(acetyloxy)methyl]-7-(formylamino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.121
(6R,7R)-3-[(acetyloxy)methyl]-7-[hydroxy(phenylacetyl)amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.49
(6R,7R)-3-[(acetyloxy)methyl]-7-[hydroxy(phenylacetyl)amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.45
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
mutant enzyme S164R
1.5
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
wild-type enzyme
1.81
(6R,7R)-3-[(acetyloxy)methyl]-8-oxo-7-[(thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
690
(6R,7R)-3-[(acetyloxy)methyl]-8-oxo-7-[(thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
-
pH and temperature not specified in the publication
0.08
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
D66W mutant, pH 7.4, room temperature
0.17
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
D66M mutant, pH 7.4, room temperature
0.19
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
D66A mutant, pH 7.4, room temperature
0.25
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
D66Y mutant, pH 7.4, room temperature
1.2
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
D66N mutant, pH 7.4, room temperature
1.6
(6R,7S)-3-[(3-carboxy-4-nitro-phenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo-[4.2.0]-oct-2-ene-2-carboxylic acid
wild type, pH 7.4, room temperature
25
3-([N-(phenylacetyl)glycyl]oxy)benzoic acid
-
pH 7.5, 25°C, strain GCI
125
3-([N-(phenylacetyl)glycyl]oxy)benzoic acid
-
pH 7.5, 25°C, strain P99
11
3-([N-[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]glycyl]oxy)benzoic acid
-
GC1 beta-lactamase
19
3-([N-[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]glycyl]oxy)benzoic acid
-
P99 beta-lactamase
11
3-[2-(2-aminothiazol-4-yl)2-(Z)-methoxyiminoacetylglycyl]oxybenzoic acid
-
pH 7.5, 25°C, strain GCI
19
3-[2-(2-aminothiazol-4-yl)2-(Z)-methoxyiminoacetylglycyl]oxybenzoic acid
-
pH 7.5, 25°C, strain P99
1.77
amoxicillin
pH 8.0, 30°C, recombinant wild-type enzyme
2.03
amoxicillin
pH 7.5, 25°C, recombinant His-tagged mutant E164A
2.38
amoxicillin
pH 7.5, 25°C, recombinant His-tagged mutant E162A
5.67
amoxicillin
-
in 100 mM MES (pH 6.4)
10
amoxicillin
30°C, pH 7.0
15.2
amoxicillin
30°C, pH not specified in the publication
21
amoxicillin
pH and temperature not specified in the publication
85
amoxicillin
at 30°C in 100 mM sodium phosphate (pH 7)
532.5
amoxicillin
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
460
amoxycillin
-
-
0.01
ampicillin
below, mutant D120C, pH 7.0, 25°C
0.01
ampicillin
below, mutant D120S, pH 7.0, 25°C
0.0126
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.06
ampicillin
30°C, pH 7.0
0.06
ampicillin
30°C, pH 7.0
0.06
ampicillin
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.065
ampicillin
30°C, pH 7.0
0.07
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.132
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.43
ampicillin
37°C, pH 7.0
0.46
ampicillin
-
at 30°C in 50 mM sodium phosphate buffer (pH 7.0)
0.53
ampicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.54
ampicillin
-
wild-type enzyme
0.55
ampicillin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
0.55
ampicillin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.55
ampicillin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.63
ampicillin
pH 7.0, 30°C
0.65
ampicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.9
ampicillin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N166A/N170G
1
ampicillin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
1
ampicillin
-
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
1
ampicillin
30°C, pH not specified in the publication
1.4
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
1.42
ampicillin
-
mutant enzyme K232N
2.12
ampicillin
-
mutant enzyme D170N
2.4
ampicillin
TEM-149 mutant T182M, pH 7.0, 25°C
2.4
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
3
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
3.2
ampicillin
-
mutant enzyme P51L
3.3
ampicillin
25°C, pH 7.0
3.3
ampicillin
pH 7.2, 25°C
5
ampicillin
pH 7.0, 30°C
5.3
ampicillin
His6-tagged wild type OXA-1, pH 7.4, room temperature
5.4
ampicillin
TEM-149 wild-type, pH 7.0, 25°C
5.9
ampicillin
beta-lactamases CTX-M-25
6.6
ampicillin
at 25°C in 100 mM sodium phosphate (pH 7.0)
6.7
ampicillin
wild type OXA-1, pH 7.4, room temperature
7
ampicillin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
8
ampicillin
30°C, pH 7.0
9.5
ampicillin
pH 7.0, 25°C
9.62
ampicillin
pH 8.0, 30°C, recombinant wild-type enzyme
10.3
ampicillin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
10.6
ampicillin
D66Y mutant, pH 7.4, room temperature
11
ampicillin
-
in 100 mM MES (pH 6.4)
12
ampicillin
beta-lactamases CTX-M-26
13
ampicillin
D66A mutant, pH 7.4, room temperature
15
ampicillin
pH 7.5, 25°C, recombinant His-tagged mutant E162A
18
ampicillin
-
wild-type enzyme
18
ampicillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
18.5
ampicillin
-
at 30°C
21.5
ampicillin
pH 7.5, 25°C, recombinant His-tagged mutant E164A
22
ampicillin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170A
32
ampicillin
-
enzyme 2, BRO-2
33
ampicillin
-
enzyme 1, BRO-1
36.9
ampicillin
37°C, pH 7.0
43
ampicillin
D66M mutant, pH 7.4, room temperature
56
ampicillin
D66W mutant, pH 7.4, room temperature
65
ampicillin
pH 7.5, 30°C
70
ampicillin
30°C, pH 7.0
77
ampicillin
mutant D120N, pH 7.0, 25°C
82
ampicillin
pH 7.5, 37°C
133
ampicillin
-
beta-lactamase D
146
ampicillin
-
mutant enzyme S164R
155
ampicillin
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
160
ampicillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
190
ampicillin
pH not specified in the publication, 30°C, recombinant enzyme
210
ampicillin
pH 7.0, 25°C
220
ampicillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
273
ampicillin
pH 7.2, 25°C, recombinant enzyme
308
ampicillin
-
beta-lactamase A
355
ampicillin
-
beta-lactamase C
357
ampicillin
-
pH and temperature not specified in the publication
357
ampicillin
-
pH and temperature not specified in the publication, OXA-1 beta-lactamase
408
ampicillin
pH 7.2, 25°C, recombinant enzyme
412
ampicillin
-
beta-lactamase B
447
ampicillin
pH 7.2, 25°C, recombinant enzyme
467
ampicillin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170G
477
ampicillin
pH 7.2, 25°C, recombinant enzyme
480
ampicillin
-
30°C, pH 7.5
480
ampicillin
D66N mutant, pH 7.4, room temperature
520
ampicillin
wild-type enzyme, pH 7.0, 25°C
520
ampicillin
wild type, pH 7.4, room temperature
544
ampicillin
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
560
ampicillin
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
724
ampicillin
pH 7.2, 25°C, recombinant enzyme
840
ampicillin
pH 7.0, 30°C, mutant R234K
900
ampicillin
pH 7.2, 25°C, recombinant enzyme
955
ampicillin
pH 7.0, 30°C
989
ampicillin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
1085
ampicillin
plasmid pBG66
-
pH and temperature not specified in the publication, wild-type enzyme
1170
ampicillin
pH 7.0, 30°C, wild-type enzyme
1375
ampicillin
pH 7.2, 25°C, recombinant enzyme
3100
ampicillin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
37
azlocillin
-
-
0.01
aztreonam
or below, pH 7.2, 25°C
0.24
aztreonam
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
0.3
aztreonam
kcat less than 0.3/sec at 30°C in 100 mM sodium phosphate (pH 7)
1.5
aztreonam
pH and temperature not specified in the publication
3.6
aztreonam
TEM-149 mutant T182M, pH 7.0, 25°C
4.7
aztreonam
TEM-149 wild-type, pH 7.0, 25°C
9
aztreonam
-
wild-type enzyme
10
aztreonam
30°C, pH 7.0
17
aztreonam
-
mutant enzyme P51L
30
aztreonam
30°C, pH 7.0
84
aztreonam
beta-lactamases CTX-M-25
100
aztreonam
beta-lactamases CTX-M-26
150
aztreonam
-
mutant enzyme S164R
162
aztreonam
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
0.004
benzylpenicillin
K226Q mutant, 30°C, pH 6.5
0.004
benzylpenicillin
V67A mutant, 30°C, pH 6.5
0.007
benzylpenicillin
H118A mutant, 30°C, pH 6.5
0.0074
benzylpenicillin
30°C, pH 7.0
0.03
benzylpenicillin
wild-type, 30°C, pH 6.5
0.03
benzylpenicillin
above, K224Q mutant, 30°C, pH 6.5
0.03
benzylpenicillin
above, V67D mutant, 30°C, pH 6.5
0.04
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.08
benzylpenicillin
V67I mutant, 30°C, pH 6.5
0.08
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.1
benzylpenicillin
in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C
0.1
benzylpenicillin
above, H196A mutant, 30°C, pH 6.5
0.13
benzylpenicillin
N116H/N220G/K224Q mutant, 30°C, pH 6.5
0.13
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.17
benzylpenicillin
mutant enzyme D120A, 1 mM Zn(NO3)2 added
0.2
benzylpenicillin
30°C, pH 7.0
0.53
benzylpenicillin
mutant enzyme D120A
0.533
benzylpenicillin
-
-
0.7
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
1
benzylpenicillin
T157A mutant, 30°C, pH 6.5
1.5
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
2.5
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
3
benzylpenicillin
30°C, pH 7.0
3.3
benzylpenicillin
N116H/N220G mutant, 30°C, pH 6.5
3.5
benzylpenicillin
TEM-149 wild-type, pH 7.0, 25°C
4
benzylpenicillin
30°C, pH 7.0
4
benzylpenicillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
4.9
benzylpenicillin
at 25°C in 100 mM sodium phosphate (pH 7.0)
10
benzylpenicillin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
10.5
benzylpenicillin
TEM-149 mutant T182M, pH 7.0, 25°C
10.92
benzylpenicillin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
11
benzylpenicillin
30°C, pH not specified in the publication
13
benzylpenicillin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
14
benzylpenicillin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
14
benzylpenicillin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
18
benzylpenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
20
benzylpenicillin
-
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
33
benzylpenicillin
beta-lactamases CTX-M-25
34.3
benzylpenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
40
benzylpenicillin
-
enzyme 1, BRO-1
41
benzylpenicillin
-
enzyme 2, BRO-2
45
benzylpenicillin
30°C, pH 7.0
48
benzylpenicillin
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
54
benzylpenicillin
pH 7.5, 30°C
55
benzylpenicillin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
75
benzylpenicillin
-
at 30°C in 50 mM sodium phosphate buffer (pH 7.0)
85
benzylpenicillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
97
benzylpenicillin
at 30°C in 100 mM sodium phosphate (pH 7)
110
benzylpenicillin
30°C, pH 7.0
120
benzylpenicillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
130
benzylpenicillin
30°C, pH 7.0
317
benzylpenicillin
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
420
benzylpenicillin
beta-lactamases CTX-M-26
650
benzylpenicillin
wild-type enzyme
650
benzylpenicillin
pH 7.0, 25°C
830
benzylpenicillin
30°C
830
benzylpenicillin
at 30°C
1010
benzylpenicillin
-
-
1160
benzylpenicillin
plasmid pQE32Chl17
-
pH 7.0, 22°C, wild-type enzyme
1350
benzylpenicillin
-
-
1400
benzylpenicillin
-
pH 7.0, 30°C
2000
benzylpenicillin
plasmid pQE32Chl17
-
pH 7.0, 22°C, mutant enzyme G240H
2200
benzylpenicillin
-
-
2650
benzylpenicillin
-
-
0.0015
biapenem
D120A mutant, 30°C, pH 6.5
0.005
biapenem
above, H263A mutant, 30°C, pH 6.5
0.1
biapenem
H118A mutant, 30°C, pH 6.5
0.26
biapenem
N116H/N220G/K224Q mutant, 30°C, pH 6.5
0.3
biapenem
mutant D120C, pH 7.0, 25°C
0.5
biapenem
mutant D120S, pH 7.0, 25°C
2.4
biapenem
above, V67LFKHV mutant, 30°C, pH 6.5
3.6
biapenem
mutant D120N, pH 7.0, 25°C
5
biapenem
N116H/N220G mutant, 30°C, pH 6.5
50
biapenem
above, V67I mutant, 30°C, pH 6.5
60
biapenem
above, K224Q mutant, 30°C, pH 6.5
100
biapenem
above, V67D mutant, 30°C, pH 6.5
100
biapenem
V67A mutant, 30°C, pH 6.5
130
biapenem
above, T157A mutant, 30°C, pH 6.5
134
biapenem
wild-type enzyme, pH 7.0, 25°C
160
biapenem
30°C, pH 6.5, mutant enzyme N220G
300
biapenem
30°C, pH 6.5, wild-type enzyme
300
biapenem
wild-type, 30°C, pH 6.5
1000
biapenem
K226Q mutant, 30°C, pH 6.5
3.72
Bocillin-FL
pH 8.0, 30°C, recombinant enzyme Y194F
12.03
Bocillin-FL
pH 8.0, 30°C, recombinant enzyme E193A
0.004
carbenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.52
carbenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
1
carbenicillin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
1.1
carbenicillin
at 25°C in 100 mM sodium phosphate (pH 7.0)
4.1
carbenicillin
25°C, pH 7.0
9.15
carbenicillin
pH 7.0, 25°C
10.22
carbenicillin
pH 8.0, 30°C, recombinant wild-type enzyme
16.96
carbenicillin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
57
carbenicillin
pH 7.0, 30°C, mutant R234K
100
carbenicillin
pH 7.0, 25°C
1200
carbenicillin
pH 7.0, 30°C, wild-type enzyme
0.28
cefaclor
mutant D120S, pH 7.0, 25°C
0.3
cefaclor
mutant D120C, pH 7.0, 25°C
6.3
cefaclor
mutant D120N, pH 7.0, 25°C
14
cefaclor
-
lactamase CoCo-L1, 25°C, pH 7.0
16
cefaclor
-
lactamase ZnFe-L1, 25°C, pH 7.0
26
cefaclor
-
lactamase ZnCo-L1, 25°C, pH 7.0
27
cefaclor
-
lactamase ZnNi-L1, 25°C, pH 7.0
29
cefaclor
-
lactamase Cu-L1, 25°C, pH 7.0
38
cefaclor
-
lactamase ZnZn-L1, 25°C, pH 7.0
42
cefaclor
wild-type enzyme, pH 7.0, 25°C
65
cefaclor
-
enzyme 1, BRO-1
69
cefaclor
-
enzyme 2, BRO-2
1020
cefaloridine
-
-
1300
cefaloridine
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
440
cefalothin
-
440
cefalothin
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
510
cefalothin
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
4.22
cefalotin
-
9
cefalotin
pH 7.2, 25°C, recombinant enzyme
18
cefalotin
pH 7.2, 25°C, recombinant enzyme
25
cefalotin
pH 7.2, 25°C, recombinant enzyme
45
cefalotin
pH 7.2, 25°C, recombinant enzyme
53
cefalotin
pH 7.2, 25°C, recombinant enzyme
63
cefalotin
pH 7.2, 25°C, recombinant enzyme
75
cefalotin
pH 7.2, 25°C, recombinant enzyme
630
cefalotin
R276H mutant, 25°C
680
cefalotin
R276G mutant, 25°C
700
cefalotin
R276C mutant, 25°C
1180
cefalotin
R276W mutant, 25°C
1190
cefalotin
CTX-M-1 wild type, 25°C
1290
cefalotin
R276N mutant, 25°C
1800
cefalotin
R276S mutant, 25°C
0.05
cefamandole
-
beta-lactamase A
0.05
cefamandole
-
beta-lactamase D
0.3
cefamandole
-
beta-lactamase C
0.35
cefamandole
-
beta-lactamase B
58.3
cefamandole
-
in 100 mM MES (pH 6.4)
0.25
cefazolin
pH 7.0, 30°C
0.46
cefazolin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
0.55
cefazolin
pH 7.0, 30°C
0.56
cefazolin
TEM-149 mutant T182M, pH 7.0, 25°C
1
cefazolin
-
beta-lactamase A
1.92
cefazolin
-
beta-lactamase D
2.2
cefazolin
TEM-149 wild-type, pH 7.0, 25°C
3.95
cefazolin
-
beta-lactamase C
4.67
cefazolin
-
beta-lactamase B
61
cefazolin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
69
cefazolin
plasmid pQE32Chl17
-
pH 7.0, 22°C, wild-type enzyme
118
cefazolin
plasmid pQE32Chl17
-
pH 7.0, 22°C, mutant enzyme G240H
325
cefazolin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
476
cefazolin
-
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
560
cefazolin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
1300
cefazolin
-
at 30°C in 50 mM sodium phosphate buffer (pH 7.0)
3000
cefazolin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.008
cefepime
30°C, pH 7.0
0.008
cefepime
D66W mutant, pH 7.4, room temperature
0.0094
cefepime
pH 7.0, 30°C
0.25
cefepime
mutant AmpC-B2-S287N
0.35
cefepime
pH 7.5, 30°C
0.43
cefepime
D66M mutant, pH 7.4, room temperature
0.43
cefepime
TEM-149 mutant T182M, pH 7.0, 25°C
0.55
cefepime
D66A mutant, pH 7.4, room temperature
0.58
cefepime
pH 7.2, 37°C, recombinant enzyme
0.64
cefepime
pH 7.0, 25°C
1.35
cefepime
mutant AmpC-A-S287N
2.4
cefepime
TEM-149 wild-type, pH 7.0, 25°C
3.8
cefepime
D66Y mutant, pH 7.4, room temperature
27
cefepime
D66N mutant, pH 7.4, room temperature
34
cefepime
wild type, pH 7.4, room temperature
40
cefepime
kcat above 40/sec at 30°C in 100 mM sodium phosphate (pH 7)
67.7
cefepime
37°C, pH 7.0
0.21
cefoperazone
pH 7.5, 25°C, recombinant His-tagged mutant E162A
0.22
cefoperazone
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
0.705
cefoperazone
pH 7.5, 25°C, recombinant His-tagged mutant E164A
229.5
cefoperazone
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
0.0002
cefotaxime
above, wild-type, 30°C, pH 6.5
0.0006
cefotaxime
K226Q mutant, 30°C, pH 6.5
0.001
cefotaxime
K224Q mutant, 30°C, pH 6.5
0.004
cefotaxime
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.01
cefotaxime
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.015
cefotaxime
pH 7.0, 30°C
0.015
cefotaxime
N116H/N220G/K224Q mutant, 30°C, pH 6.5
0.023
cefotaxime
T157A mutant, 30°C, pH 6.5
0.032
cefotaxime
30°C, pH 7.0
0.032
cefotaxime
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.045
cefotaxime
mutant AmpC-B2-S287N
0.05
cefotaxime
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
0.07
cefotaxime
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.07
cefotaxime
TEM-149 wild-type, pH 7.0, 25°C
0.08
cefotaxime
mutant AmpC-A-S287N
0.09
cefotaxime
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.1
cefotaxime
wild-type AmpC-B2
0.2
cefotaxime
wild-type AmpC-A
0.3
cefotaxime
30°C, pH 7.0
0.3
cefotaxime
N116H/N220G mutant, 30°C, pH 6.5
0.31
cefotaxime
D66M mutant, pH 7.4, room temperature
0.37
cefotaxime
37°C, pH 7.0
0.37
cefotaxime
D66A mutant, pH 7.4, room temperature
0.41
cefotaxime
-
pH 7.5, 25°C, strain P99
0.41
cefotaxime
-
P99 beta-lactamase
0.41
cefotaxime
TEM-149 mutant T182M, pH 7.0, 25°C
0.45
cefotaxime
-
mutant enzyme S164R
0.7
cefotaxime
pH 7.5, 30°C
0.71
cefotaxime
D66Y mutant, pH 7.4, room temperature
0.74
cefotaxime
plasmid pQE32Chl17
-
pH 7.0, 22°C, wild-type enzyme
1.1
cefotaxime
25°C, pH 7.0
1.5
cefotaxime
-
wild-type enzyme
1.53
cefotaxime
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
1.572
cefotaxime
pH 8.0, 30°C, recombinant wild-type enzyme
2.7
cefotaxime
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
2.7
cefotaxime
plasmid pQE32Chl17
-
pH 7.0, 22°C, mutant enzyme G240H
2.9
cefotaxime
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
3
cefotaxime
pH 7.0, 30°C
3.3
cefotaxime
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
3.5
cefotaxime
D66N mutant, pH 7.4, room temperature
5.3
cefotaxime
wild type, pH 7.4, room temperature
8.3
cefotaxime
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
20
cefotaxime
30°C, pH 7.0
21
cefotaxime
R276G mutant, 25°C
22
cefotaxime
at 30°C in 100 mM sodium phosphate (pH 7)
30
cefotaxime
30°C, pH 7.0
33
cefotaxime
pH 7.5, 37°C
43
cefotaxime
plasmid pBC
-
pH 7.4, 22°C, T237S mutant enzyme
44
cefotaxime
R276S mutant, 25°C
56
cefotaxime
pH not specified in the publication, 30°C, recombinant enzyme
62
cefotaxime
-
pH 7.5, 25°C, strain GCI
62
cefotaxime
-
GC1 beta-lactamase
80
cefotaxime
-
30°C, pH 7.5
91
cefotaxime
R276C mutant, 25°C
92
cefotaxime
R276W mutant, 25°C
101
cefotaxime
beta-lactamases CTX-M-25
107
cefotaxime
R276N mutant, 25°C
120
cefotaxime
beta-lactamases CTX-M-26
120
cefotaxime
R276H mutant, 25°C
140
cefotaxime
CTX-M-1 wild type, 25°C
142
cefotaxime
plasmid pBC
-
pH 7.4, 22°C, wild-type enzyme
197
cefotaxime
37°C, pH 7.0
398
cefotaxime
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
440
cefotaxime
wild type OXA-1, pH 7.4, room temperature
452
cefotaxime
pH and temperature not specified in the publication
520
cefotaxime
His6-tagged wild type OXA-1, pH 7.4, room temperature
1400
cefotaxime
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
0.00043
cefoxitin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
0.01
cefoxitin
kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7)
0.032
cefoxitin
pH 7.0, 25°C
0.038
cefoxitin
30°C, pH 7.0
0.043
cefoxitin
37°C, pH 7.0
0.05
cefoxitin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.08
cefoxitin
mutant AmpC-B2-S287N
0.1
cefoxitin
mutant AmpC-A-S287N
0.23
cefoxitin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.27
cefoxitin
pH 7.2, 37°C, recombinant enzyme
0.3
cefoxitin
30°C, pH 7.0
0.3
cefoxitin
pH 7.2, 25°C
0.3
cefoxitin
wild-type AmpC-A
0.34
cefoxitin
mutant D120S, pH 7.0, 25°C
0.37
cefoxitin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
0.4
cefoxitin
wild-type AmpC-B2
0.56
cefoxitin
mutant D120C, pH 7.0, 25°C
0.64
cefoxitin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
0.8
cefoxitin
-
in 100 mM MES (pH 6.4)
0.88
cefoxitin
pH 7.0, 30°C
1.53
cefoxitin
mutant D120N, pH 7.0, 25°C
1.9
cefoxitin
wild-type enzyme, pH 7.0, 25°C
3
cefoxitin
pH 7.2, 25°C, recombinant enzyme
4
cefoxitin
pH 7.2, 25°C, recombinant enzyme
4.2
cefoxitin
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
6
cefoxitin
pH 7.2, 25°C, recombinant enzyme
7
cefoxitin
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
8.3
cefoxitin
25°C, pH 7.0
9.6
cefoxitin
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
10
cefoxitin
pH 7.2, 25°C, recombinant enzyme
14
cefoxitin
pH 7.2, 25°C, recombinant enzyme
18
cefoxitin
pH 7.2, 25°C, recombinant enzyme
35
cefoxitin
pH 7.5, 37°C
0.1
cefpirome
30°C, pH 7.0
1.5
cefpirome
37°C, pH 7.0
23
cefpirome
pH 7.0, 30°C
95
cefpirome
at 30°C in 100 mM sodium phosphate (pH 7)
0.00833
ceftazidime
-
-
0.01
ceftazidime
mutant AmpC-B2-S287N
0.015
ceftazidime
beta-lactamases CTX-M-26
0.033
ceftazidime
-
in 100 mM MES (pH 6.4)
0.04
ceftazidime
30°C, pH 7.0
0.07
ceftazidime
pH 7.2, 25°C
0.084
ceftazidime
37°C, pH 7.0
0.09
ceftazidime
pH 7.2, 37°C, recombinant enzyme
0.11
ceftazidime
pH 7.0, 30°C
0.2
ceftazidime
-
at 30°C
0.2
ceftazidime
mutant AmpC-A-S287N
0.3
ceftazidime
wild-type AmpC-B2
0.5
ceftazidime
wild-type AmpC-A
1
ceftazidime
pH 7.5, 30°C
1
ceftazidime
30°C, pH 7.0
1.3
ceftazidime
pH and temperature not specified in the publication
1.5
ceftazidime
30°C, pH 7.0
2 - 8
ceftazidime
pH 7.2, 25°C, recombinant enzyme
2
ceftazidime
30°C, pH 7.0
2.1
ceftazidime
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
3.3
ceftazidime
kcat above than 3.3/sec at 30°C in 100 mM sodium phosphate (pH 7)
5
ceftazidime
pH 7.2, 25°C, recombinant enzyme
8.3
ceftazidime
TEM-149 mutant T182M, pH 7.0, 25°C
8.3
ceftazidime
TEM-149 wild-type, pH 7.0, 25°C
9
ceftazidime
pH 7.5, 37°C
12
ceftazidime
-
wild-type enzyme
12
ceftazidime
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
15
ceftazidime
pH 7.0, 30°C
15
ceftazidime
pH 7.2, 25°C, recombinant enzyme
16
ceftazidime
pH 7.2, 25°C, recombinant enzyme
18
ceftazidime
25°C, pH 7.0
20
ceftazidime
-
30°C, pH 7.5
23
ceftazidime
pH 7.2, 25°C, recombinant enzyme
24
ceftazidime
pH 7.2, 25°C, recombinant enzyme
29
ceftazidime
pH 7.2, 25°C, recombinant enzyme
33
ceftazidime
beta-lactamases CTX-M-25
60
ceftazidime
-
mutant enzyme P51L
0.042
ceftriaxone
pH 7.0, 30°C
0.82
ceftriaxone
-
in 100 mM MES (pH 6.4)
1.825
ceftriaxone
pH 8.0, 30°C, recombinant wild-type enzyme
25
ceftriaxone
30°C, pH 7.0
30
ceftriaxone
at 30°C in 100 mM sodium phosphate (pH 7)
0.00833
cefuroxime
-
beta-lactamase A
0.017
cefuroxime
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.02
cefuroxime
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.02
cefuroxime
wild-type, pH 7.0, 22°C
0.042
cefuroxime
pH 7.0, 30°C
0.1
cefuroxime
pH 7.2, 25°C
0.12
cefuroxime
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
0.28
cefuroxime
-
pH 7.5, 25°C, strain P99
0.28
cefuroxime
-
P99 beta-lactamase
0.9
cefuroxime
pH 7.5, 30°C
1
cefuroxime
30°C, pH 7.0
2
cefuroxime
pH 7.0, 30°C
2.5
cefuroxime
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
3.4
cefuroxime
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
4.1
cefuroxime
-
enzyme 1, BRO-1
4.8
cefuroxime
-
enzyme 2, BRO-2
5.9
cefuroxime
25°C, pH 7.0
8.17
cefuroxime
-
in 100 mM MES (pH 6.4)
10
cefuroxime
30°C, pH 7.0
11
cefuroxime
pH 7.2, 37°C, recombinant enzyme
15
cefuroxime
at 30°C in 100 mM sodium phosphate (pH 7)
21
cefuroxime
beta-lactamases CTX-M-26
23
cefuroxime
pH 7.5, 37°C
74.8
cefuroxime
-
GC1 beta-lactamase
120
cefuroxime
pH 7.0, 25°C
140
cefuroxime
-
30°C, pH 7.5
170
cefuroxime
30°C, pH 7.0
190
cefuroxime
beta-lactamases CTX-M-25
286
cefuroxime
pH and temperature not specified in the publication
0.42
CENTA
at 30°C
29.5
CENTA
-
in 100 mM MES (pH 6.4)
0.079
cephalexin
pH 8.0, 30°C, recombinant wild-type enzyme
1
cephalexin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170A
4.83
cephalexin
-
pH 7.0, 25°C
13
cephalexin
plasmid pBG66
-
pH and temperature not specified in the publication, wild-type enzyme
16
cephalexin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170G
26
cephalexin
-
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
31
cephalexin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
55
cephalexin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
55
cephalexin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
72
cephalexin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
80
cephalexin
-
at 30°C in 50 mM sodium phosphate buffer (pH 7.0)
85
cephalexin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
115
cephalexin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
160
cephalexin
pH 7.0, 30°C
0.002
cephaloridine
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.0035
cephaloridine
mutant enzyme D120A, 1 mM Zn(NO3)2 added
0.0036
cephaloridine
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.004
cephaloridine
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.006
cephaloridine
below, wild-type, 30°C, pH 6.5
0.006
cephaloridine
K226Q mutant, 30°C, pH 6.5
0.01
cephaloridine
above, H118A mutant, 30°C, pH 6.5
0.015
cephaloridine
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.022
cephaloridine
mutant enzyme D120E, 1 mM Zn(NO3)2 added
0.024
cephaloridine
K224Q mutant, 30°C, pH 6.5
0.03
cephaloridine
N116H/N220G/K224Q mutant, 30°C, pH 6.5
0.05
cephaloridine
30°C, pH 7.0
0.11
cephaloridine
above, H196A mutant, 30°C, pH 6.5
0.22
cephaloridine
mutant enzyme D120E
0.23
cephaloridine
mutant enzyme D120A
0.9
cephaloridine
N116H/N220G mutant, 30°C, pH 6.5
1.25
cephaloridine
-
beta-lactamase D
1.3
cephaloridine
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
1.95
cephaloridine
-
beta-lactamase A
4.8
cephaloridine
-
enzyme 1, BRO-1
5.6
cephaloridine
-
enzyme 2, BRO-2
5.6
cephaloridine
-
beta-lactamase C
6.65
cephaloridine
-
beta-lactamase B
10
cephaloridine
30°C, pH 7.0
13
cephaloridine
-
wild-type enzyme
15
cephaloridine
-
wild-type enzyme
16
cephaloridine
-
pH and temperature not specified in the publication
16
cephaloridine
-
pH and temperature not specified in the publication, OXA-1 beta-lactamase
22
cephaloridine
-
mutant enzyme P51L
30
cephaloridine
30°C, pH 7.0
35
cephaloridine
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
50
cephaloridine
mutant AmpC-A-S287N
64
cephaloridine
37°C, pH 7.0
70
cephaloridine
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
90
cephaloridine
wild-type enzyme
112
cephaloridine
mutant AmpC-B2-S287N
115
cephaloridine
at 30°C in 100 mM sodium phosphate (pH 7)
127
cephaloridine
-
mutant enzyme K104E
155
cephaloridine
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
190
cephaloridine
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
215
cephaloridine
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
215
cephaloridine
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
230
cephaloridine
wild-type AmpC-A
240
cephaloridine
wild-type AmpC-B2
590
cephaloridine
-
mutant enzyme S164R
0.2
cephalosporin C
-
-
4.7
cephalosporin C
-
enzyme 2, BRO-2
5
cephalosporin C
-
enzyme 1, BRO-1
17.83
cephalosporin C
-
in 100 mM MES (pH 6.4)
0.0023
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.003
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.003
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.004
cephalothin
30°C, pH 7.0
0.00667
cephalothin
-
beta-lactamase D
0.012
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.0167
cephalothin
-
beta-lactamase A
0.1
cephalothin
-
beta-lactamase B
0.1
cephalothin
-
beta-lactamase C
0.1
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.15
cephalothin
D66W mutant, pH 7.4, room temperature
0.15
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
0.2
cephalothin
30°C, pH not specified in the publication
0.27
cephalothin
D66A mutant, pH 7.4, room temperature
0.28
cephalothin
-
wild-type enzyme
0.28
cephalothin
D66Y mutant, pH 7.4, room temperature
0.31
cephalothin
mutant D120C, pH 7.0, 25°C
0.37
cephalothin
mutant D120S, pH 7.0, 25°C
0.38
cephalothin
D66M mutant, pH 7.4, room temperature
0.43
cephalothin
pH 7.5, 25°C, recombinant His-tagged mutant E164A
0.44
cephalothin
pH 7.5, 25°C, recombinant His-tagged mutant E162A
0.5
cephalothin
in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C
0.6
cephalothin
30°C, pH 7.0
0.65
cephalothin
-
mutant enzyme K232N
0.86
cephalothin
-
mutant enzyme D170N
1.031
cephalothin
pH 8.0, 30°C, recombinant wild-type enzyme
1.6
cephalothin
D66N mutant, pH 7.4, room temperature
2
cephalothin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
2.5
cephalothin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2.7
cephalothin
wild type, pH 7.4, room temperature
4.6
cephalothin
-
enzyme 2, BRO-2
4.9
cephalothin
-
enzyme 1, BRO-1
6
cephalothin
30°C, pH 7.0
6
cephalothin
in 20 mM phosphate buffer, (pH 7.0) at 30°C
8
cephalothin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
8.1
cephalothin
mutant D120N, pH 7.0, 25°C
10
cephalothin
mutant AmpC-B2-S287N
10
cephalothin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
11
cephalothin
-
GC1 beta-lactamase
12.1
cephalothin
-
at 30°C
14
cephalothin
30°C, pH 7.0
14
cephalothin
in 20 mM phosphate buffer, (pH 7.0) at 30°C
14
cephalothin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
16
cephalothin
25°C, pH 7.0
22
cephalothin
at 30°C in 100 mM sodium phosphate (pH 7)
26
cephalothin
plasmid pBC
-
pH 7.4, 22°C, T237A mutant enzyme
31
cephalothin
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170G
44
cephalothin
pH 7.0, 30°C
44
cephalothin
plasmid pBC
-
pH 7.4, 22°C, wild-type enzyme
49.7
cephalothin
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
56
cephalothin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
60
cephalothin
pH not specified in the publication, 30°C, recombinant enzyme
63
cephalothin
plasmid pQE32Chl17
-
pH 7.0, 22°C, mutant enzyme G240H
65
cephalothin
mutant AmpC-A-S287N
65
cephalothin
plasmid pBC
-
pH 7.4, 22°C, T237S mutant enzyme
74.8
cephalothin
-
pH 7.5, 25°C, strain GCI
77
cephalothin
plasmid pQE32Chl17
-
pH 7.0, 22°C, wild-type enzyme
82
cephalothin
wild-type enzyme, pH 7.0, 25°C
113
cephalothin
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
140
cephalothin
30°C, pH 7.0
140
cephalothin
in 20 mM phosphate buffer, (pH 7.0) at 30°C
146
cephalothin
plasmid pBG66
-
pH and temperature not specified in the publication, wild-type enzyme
150
cephalothin
30°C, pH 7.0
160
cephalothin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
160
cephalothin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
185
cephalothin
37°C, pH 7.0
230
cephalothin
beta-lactamases CTX-M-25
280
cephalothin
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
300
cephalothin
30°C, pH 7.0
400
cephalothin
30°C, pH 7.0
460
cephalothin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
470
cephalothin
pH 7.0, 30°C
480
cephalothin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
500
cephalothin
wild-type AmpC-A
530
cephalothin
beta-lactamases CTX-M-26
540
cephalothin
wild-type AmpC-B2
543
cephalothin
pH and temperature not specified in the publication
690
cephalothin
-
pH 7.5, 25°C, strain P99
690
cephalothin
-
P99 beta-lactamase
0.2
cephalotin
30°C, pH 7.0
0.4
cephalotin
pH 7.5, 30°C
1
cephalotin
pH 7.0, 30°C
8.17
cephalotin
-
in 100 mM MES (pH 6.4)
25
cephalotin
pH 7.5, 37°C
125
cephalotin
-
pH 7.0, 30°C
500
cephalotin
pH 7.0, 25°C
0.25
cephapirin
-
beta-lactamase D
0.367
cephapirin
-
beta-lactamase A
1.18
cephapirin
-
beta-lactamase C
1.82
cephapirin
-
beta-lactamase B
0.004
Cloxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.2
Cloxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.42
Cloxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.5
Cloxacillin
30°C, pH 7.0
0.51
Cloxacillin
-
wild-type enzyme
1.04
Cloxacillin
-
mutant enzyme K232N
1.12
Cloxacillin
-
mutant enzyme D170N
1.8
Cloxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2.5
Cloxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
3.5
Cloxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
4
Cloxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
5
Cloxacillin
-
enzyme 1, BRO-1
8.6
Cloxacillin
-
enzyme 2, BRO-2
120
Cloxacillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
500
Cloxacillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.00033
doripenem
pH 6.5, 22°C, recombinant enzyme
0.04
doripenem
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
51
doripenem
pH not specified in the publication, 30°C, recombinant enzyme
75
doripenem
pH 7.2, 25°C, recombinant enzyme
170
doripenem
pH 7.2, 25°C, recombinant enzyme
226
doripenem
pH 7.2, 25°C, recombinant enzyme
267
doripenem
pH 7.2, 25°C, recombinant enzyme
348
doripenem
pH 7.2, 25°C, recombinant enzyme
743
doripenem
pH 7.2, 25°C, recombinant enzyme
1032
doripenem
pH 7.2, 25°C, recombinant enzyme
0.00033
ertapenem
pH 6.5, 22°C, recombinant enzyme
0.03
ertapenem
pH 7.5, 30°C
0.13
ertapenem
pH 7.0, 30°C
0.4
ertapenem
pH 7.2, 25°C
1.8
ertapenem
pH 7.5, 37°C
4
ertapenem
pH 7.2, 37°C, recombinant enzyme
4.3
ertapenem
pH 7.2, 37°C, recombinant enzyme, in presence of NaCl
69
ertapenem
pH not specified in the publication, 30°C, recombinant enzyme
0.038
faropenem
pH 7.0, 30°C
0.00024
Imipenem
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
0.002
Imipenem
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.003
Imipenem
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
0.011
Imipenem
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
0.012
Imipenem
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
0.02
Imipenem
H263A mutant, 30°C, pH 6.5
0.024
Imipenem
D120A mutant, 30°C, pH 6.5
0.025
Imipenem
D120T mutant, 30°C, pH 6.5
0.028
Imipenem
-
at 40?C in 50 mM HEPES buffer (pH 6.8)
0.033
Imipenem
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.06
Imipenem
at 30°C in 100 mM sodium phosphate (pH 7)
0.06
Imipenem
mutant AmpC-A-S287N
0.06
Imipenem
mutant AmpC-B2-S287N
0.07
Imipenem
30°C, pH 7.0
0.07
Imipenem
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.09
Imipenem
wild-type AmpC-B2
0.09
Imipenem
30°C, pH not specified in the publication
0.1
Imipenem
30°C, pH 7.0
0.1
Imipenem
30°C, pH 7.0
0.1
Imipenem
30°C, pH 7.0
0.1
Imipenem
30°C, pH 7.0
0.1
Imipenem
wild-type AmpC-A
0.11
Imipenem
30°C, pH 7.0
0.11
Imipenem
in 20 mM phosphate buffer, (pH 7.0) at 30°C
0.167
Imipenem
-
in 100 mM MES (pH 6.4)
0.36
Imipenem
mutant D120C, pH 7.0, 25°C
0.63
Imipenem
mutant D120S, pH 7.0, 25°C
0.9
Imipenem
N116H/N220G/K224Q mutant, 30°C, pH 6.5
1.2
Imipenem
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
1.7
Imipenem
pH 7.2, 25°C
2.03
Imipenem
mutant enzyme D120A
2.4
Imipenem
H118A mutant, 30°C, pH 6.5
3.17
Imipenem
mutant enzyme D120A, 1 mM Zn(NO3)2 added
3.7
Imipenem
mutant enzyme D120E
4.8
Imipenem
pH 7.0, 30°C
6.8
Imipenem
plasmid pBC
-
pH 7.4, 22°C, T237A mutant enzyme
7.28
Imipenem
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
8
Imipenem
pH 7.2, 37°C, recombinant enzyme
12
Imipenem
-
lactamase ZnCo-L1, 25°C, pH 7.0
13
Imipenem
-
lactamase ZnZn-L1, 25°C, pH 7.0
15
Imipenem
mutant D120N, pH 7.0, 25°C
16
Imipenem
H196A mutant, 30°C, pH 6.5
16
Imipenem
N116H/N220G mutant, 30°C, pH 6.5
19
Imipenem
plasmid pBC
-
pH 7.4, 22°C, wild-type enzyme
21
Imipenem
plasmid pBC
-
pH 7.4, 22°C, T237S mutant enzyme
30
Imipenem
above, V67LFKHV mutant, 30°C, pH 6.5
43
Imipenem
-
lactamase CoCo-L1, 25°C, pH 7.0
49
Imipenem
wild type enzyme, at pH 9.0
54
Imipenem
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
54
Imipenem
pH 7.2, 25°C, recombinant enzyme
59
Imipenem
-
lactamase ZnFe-L1, 25°C, pH 7.0
120
Imipenem
pH 7.5, 37°C
120
Imipenem
-
30°C, pH 7.5
123.3
Imipenem
wild-type enzyme
127
Imipenem
pH 7.2, 25°C, recombinant enzyme
140
Imipenem
wild type enzyme, at pH 8.0
166
Imipenem
-
lactamase ZnNi-L1, 25°C, pH 7.0
205
Imipenem
-
lactamase Cu-L1, 25°C, pH 7.0
210
Imipenem
wild type enzyme, at pH 7.0
214
Imipenem
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
252
Imipenem
pH 7.2, 25°C, recombinant enzyme
292
Imipenem
E152C mutant, pH 7.0, 25°C
295
Imipenem
spin-labeled enzyme, pH 7.0, 25°C
312
Imipenem
pH not specified in the publication, 30°C, recombinant enzyme
332
Imipenem
pH 7.2, 25°C, recombinant enzyme
355
Imipenem
pH 7.2, 25°C, recombinant enzyme
370
Imipenem
wild-type enzyme, pH 7.0, 25°C
390
Imipenem
30°C, pH 6.5, mutant enzyme N220G
490
Imipenem
above, K224Q mutant, 30°C, pH 6.5
600
Imipenem
pH 7.2, 25°C, recombinant enzyme
757
Imipenem
pH 7.2, 25°C, recombinant enzyme
1000
Imipenem
K226Q mutant, 30°C, pH 6.5
1000
Imipenem
V67A mutant, 30°C, pH 6.5
1200
Imipenem
30°C, pH 6.5, wild-type enzyme
1200
Imipenem
wild-type, 30°C, pH 6.5
1280
Imipenem
V67D mutant, 30°C, pH 6.5
1680
Imipenem
T157A mutant, 30°C, pH 6.5
1770
Imipenem
V67I mutant, 30°C, pH 6.5
79
Loracarbef
-
enzyme 1, BRO-1
95
Loracarbef
-
enzyme 2, BRO-2
29
mecillinam
-
enzyme 2, BRO-2
31
mecillinam
-
enzyme 1, BRO-1
0.0013
meropenem
-
in 100 mM MES (pH 6.4)
0.0013
meropenem
pH 6.5, 22°C, recombinant enzyme
0.007
meropenem
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
0.01
meropenem
30°C, pH not specified in the publication
0.05
meropenem
pH 7.5, 30°C
0.05
meropenem
30°C, pH 7.0
0.06
meropenem
30°C, pH 7.0
0.07
meropenem
pH 7.0, 30°C
0.29
meropenem
mutant D120C, pH 7.0, 25°C
0.75
meropenem
mutant D120S, pH 7.0, 25°C
1.4
meropenem
pH 7.5, 37°C
1.4
meropenem
pH 7.2, 25°C
2.7
meropenem
25°C, pH 7.0
4.13
meropenem
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
6.5
meropenem
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
10.6
meropenem
mutant D120N, pH 7.0, 25°C
13
meropenem
pH 7.2, 37°C, recombinant enzyme
40
meropenem
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
109
meropenem
pH 7.0, 25°C
142
meropenem
pH 7.2, 25°C, recombinant enzyme
157
meropenem
wild-type enzyme, pH 7.0, 25°C
194
meropenem
pH 7.2, 25°C, recombinant enzyme
200
meropenem
-
30°C, pH 7.5
220
meropenem
pH not specified in the publication, 30°C, recombinant enzyme
238
meropenem
pH 7.2, 25°C, recombinant enzyme
301
meropenem
pH 7.2, 25°C, recombinant enzyme
362
meropenem
pH 7.2, 25°C, recombinant enzyme
413
meropenem
pH 7.2, 25°C, recombinant enzyme
583
meropenem
pH 7.2, 25°C, recombinant enzyme
145
Methicillin
-
-
168
Methicillin
-
enzyme 1, BRO-1
190
Methicillin
-
enzyme 2, BRO-2
0.01
moxalactam
kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7)
0.017
moxalactam
pH 7.0, 25°C
14
moxalactam
25°C, pH 7.0
0.0002
nitrocefin
D120T mutant, 30°C, pH 6.5
0.0024
nitrocefin
D120A mutant, 30°C, pH 6.5
0.003
nitrocefin
H263A mutant, 30°C, pH 6.5
0.004
nitrocefin
V67D mutant, 30°C, pH 6.5
0.004
nitrocefin
V67I mutant, 30°C, pH 6.5
0.005
nitrocefin
H118A mutant, 30°C, pH 6.5
0.005
nitrocefin
V67A mutant, 30°C, pH 6.5
0.006
nitrocefin
H196A mutant, 30°C, pH 6.5
0.006
nitrocefin
above, V67LFKHV mutant, 30°C, pH 6.5
0.007
nitrocefin
K224Q mutant, 30°C, pH 6.5
0.008
nitrocefin
wild-type, 30°C, pH 6.5
0.01
nitrocefin
K226Q mutant, 30°C, pH 6.5
0.014
nitrocefin
pH 8.0, 30°C, recombinant wild-type enzyme
0.015
nitrocefin
T157A mutant, 30°C, pH 6.5
0.028
nitrocefin
mutant D120S, pH 7.0, 25°C
0.06
nitrocefin
pH 7.0, 25°C
0.08
nitrocefin
pH 7.5, 25°C, recombinant His-tagged mutant E162A
0.125
nitrocefin
pH 7.5, 25°C, recombinant His-tagged mutant E164A
0.18
nitrocefin
mutant D120C, pH 7.0, 25°C
0.2
nitrocefin
N116H/N220G/K224Q mutant, 30°C, pH 6.5
0.27
nitrocefin
mutant enzyme D120A
0.4
nitrocefin
30°C, pH 7.0
0.55
nitrocefin
mutant enzyme D120E
0.6
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
0.7
nitrocefin
N116H/N220G mutant, 30°C, pH 6.5
1
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
2
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2.5
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2.54
nitrocefin
mutant D120N, pH 7.0, 25°C
2.6
nitrocefin
lactamase 1Fe-L1, 0.9 Co(2+), 0.20 Zn(2+)
3 - 6
nitrocefin
-
lactamase NiZn-L1, 25°C, pH 7.0
4
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
4.17
nitrocefin
mutant enzyme D120E, 1 mM Zn(NO3)2 added
4.4
nitrocefin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
5
nitrocefin
-
wild-type enzyme
5.8
nitrocefin
25°C, pH 7.0
5.85
nitrocefin
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
6
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
6.3
nitrocefin
pH 7.2, 37°C, recombinant enzyme
7.4
nitrocefin
TEM-149 wild-type, pH 7.0, 25°C
7.5
nitrocefin
mutant enzyme D120A, 1 mM Zn(NO3)2 added
8
nitrocefin
-
mutant enzyme P51L
8
nitrocefin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
11
nitrocefin
lactamase 1Co-L1, 0.9 Co(2+), 0.10 Zn(2+)
13.7
nitrocefin
-
beta-lactamase C
15
nitrocefin
pH 7.2, 25°C, recombinant enzyme
16.5
nitrocefin
-
beta-lactamase B
20
nitrocefin
lactamase ZnFe-L1, 1.0 Zn(2+), 1.0 Fe(3+)
23.2
nitrocefin
-
beta-lactamase A
24
nitrocefin
-
30°C, pH 8.0
24
nitrocefin
-
lactamase Ni-L1, 25°C, pH 7.0
24
nitrocefin
lactamase ZnFe-L1, 1.2 Zn(2+), 0.9 Fe(3+)
25.6
nitrocefin
-
beta-lactamase D
26
nitrocefin
lactamase ZnCo-L1, 1.0 Zn(2+), 1.0 Co(2+)
26
nitrocefin
TEM-149 mutant T182M, pH 7.0, 25°C
29
nitrocefin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
29.7
nitrocefin
-
mutant enzyme K232N
30
nitrocefin
pH 7.2, 25°C, recombinant enzyme
30
nitrocefin
lactamase 1Zn-L1, 1.0 Zn(2+)
33
nitrocefin
pH 7.2, 25°C, recombinant enzyme
38
nitrocefin
pH 7.2, 25°C, recombinant enzyme
39
nitrocefin
lactamase ZnZn-L1, 2.0 Zn(2+) added
41
nitrocefin
wild-type enzyme, pH 7.0, 25°C
44
nitrocefin
pH 7.2, 25°C, recombinant enzyme
49
nitrocefin
pH 7.2, 25°C, recombinant enzyme
50
nitrocefin
plasmid pBC
-
pH 7.4, 22°C, T237S mutant enzyme
52
nitrocefin
plasmid pBC
-
pH 7.4, 22°C, wild-type enzyme
55
nitrocefin
pH 7.5, 30°C
63
nitrocefin
-
wild-type enzyme
63
nitrocefin
lactamase CoCo-L1, 1.80 Co(2+)
65
nitrocefin
pH 7.2, 25°C, recombinant enzyme
72
nitrocefin
pH 7.0, 30°C
79.5
nitrocefin
-
mutant enzyme D170N
93
nitrocefin
30°C, pH 7.0
96
nitrocefin
-
lactamase Cu-L1, 25°C, pH 7.0
96
nitrocefin
-
pH and temperature not specified in the publication
96
nitrocefin
-
pH and temperature not specified in the publication, OXA-1 beta-lactamase
100
nitrocefin
30°C, pH 7.0
111.3
nitrocefin
-
in 100 mM MES (pH 6.4)
135
nitrocefin
pH 7.5, 25°C
137
nitrocefin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
151
nitrocefin
-
enzyme 1, BRO-1
176
nitrocefin
-
mutant enzyme K104E
191
nitrocefin
plasmid pBC
-
pH 7.4, 22°C, T237A mutant enzyme
233
nitrocefin
-
enzyme 2, BRO-2
237
nitrocefin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
252
nitrocefin
37°C, pH 7.0
330
nitrocefin
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
360
nitrocefin
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
400
nitrocefin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
466.7
nitrocefin
wild-type enzyme
535
nitrocefin
wild-type enzyme
550
nitrocefin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
600
nitrocefin
-
mutant enzyme S164R
690
nitrocefin
pH 7.0, 25°C
765
nitrocefin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
765
nitrocefin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
780
nitrocefin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
940
nitrocefin
pH 7.0, 30°C
1240
nitrocefin
-
at 30°C in 50 mM sodium phosphate buffer (pH 7.0)
1300
nitrocefin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
1407
nitrocefin
-
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
1700
nitrocefin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2220
nitrocefin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.008
Oxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.009
Oxacillin
-
30°C, pH 7.0, plasmid-encoded, pBC3e, beta-lactamase CMY-1
0.015
Oxacillin
-
30°C, pH 7.0, plasmid-encoded, pBC4e, beta-lactamase CMY-2
0.022
Oxacillin
30°C, pH 7.0
0.044
Oxacillin
-
30°C, pH 7.0, plasmid-encoded, pBC1e, beta-lactamase ACT-1
0.18
Oxacillin
-
30°C, pH 7.0, plasmid-encoded, pBC2e, beta-lactamase MIR-1
0.2
Oxacillin
30°C, pH 7.0
0.21
Oxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.25
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
1.23
Oxacillin
-
wild-type enzyme
2
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
2.37
Oxacillin
-
mutant enzyme D170N
3.07
Oxacillin
-
mutant enzyme K232N
3.5
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
4
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
4.2
Oxacillin
30°C, pH not specified in the publication
4.5
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
11.9
Oxacillin
pH 8.0, 30°C, recombinant wild-type enzyme
35
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
90
Oxacillin
plasmid pGEM-T-easy
-
mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
113
Oxacillin
at 25°C in 100 mM sodium phosphate (pH 7.0)
130
Oxacillin
pH 7.0, 30°C
300
Oxacillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate
577
Oxacillin
-
pH and temperature not specified in the publication
577
Oxacillin
-
pH and temperature not specified in the publication, OXA-1 beta-lactamase
600
Oxacillin
plasmid pGEM-T-easy
-
wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate
1.4
panipenem
pH 7.0, 30°C
500
panipenem
pH not specified in the publication, 30°C, recombinant enzyme
6.6
penicillin
25°C, pH 7.0
0.01
penicillin G
below, mutant D120C, pH 7.0, 25°C
0.01
penicillin G
below, mutant D120S, pH 7.0, 25°C
0.32
penicillin G
mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C
0.4
penicillin G
-
wild-type enzyme
0.61
penicillin G
-
mutant enzyme D170N
0.75
penicillin G
-
mutant enzyme K232N
0.8
penicillin G
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N166A/N170G
2.1
penicillin G
mutant E166C, pH 7.0, 22°C
9.33
penicillin G
-
in 100 mM MES (pH 6.4)
12.3
penicillin G
-
mutant R61H/E64H/43H, 25°C, pH not specified in the publication
23
penicillin G
-
mutant enzyme P51L
24.8
penicillin G
-
mutant M182T/T195S/A224V, 25°C, pH not specified in the publication
26
penicillin G
-
wild-type enzyme
27
penicillin G
pH 7.0, 30°C
27.6
penicillin G
pH 8.0, 30°C, recombinant wild-type enzyme
31
penicillin G
pH and temperature not specified in the publication
33
penicillin G
-
mutant enzyme K104E
34
penicillin G
-
at 40?C in 50 mM HEPES buffer (pH 6.8)
45
penicillin G
mutant D120N, pH 7.0, 25°C
45
penicillin G
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170A
46
penicillin G
-
beta-lactamase D
64
penicillin G
plasmid pBG66
-
pH and temperature not specified in the publication, mutant enzyme N170G
118
penicillin G
-
lactamase CoCo-L1, 25°C, pH 7.0
129
penicillin G
-
wild-type, 25°C, pH not specified in the publication
156
penicillin G
-
beta-lactamase B
171
penicillin G
-
beta-lactamase A
200
penicillin G
-
mutant enzyme S164R
210
penicillin G
-
beta-lactamase C
430
penicillin G
pH 7.0, 30°C, mutant R234K
600
penicillin G
wild-type enzyme, pH 7.0, 25°C
628
penicillin G
-
pH 7.0, 25°C
692
penicillin G
-
lactamase ZnCo-L1, 25°C, pH 7.0
761
penicillin G
-
lactamase ZnZn-L1, 25°C, pH 7.0
890
penicillin G
pH 7.0, 30°C, wild-type enzyme
950
penicillin G
plasmid pBG66
-
pH and temperature not specified in the publication, wild-type enzyme
2612
penicillin G
wild-type, pH 7.0, 22°C
0.2
piperacillin
30°C, pH 7.0
0.5
piperacillin
in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C
0.54
piperacillin
pH 7.0, 30°C
1.5
piperacillin
30°C, pH 7.0
2
piperacillin
30°C, pH 7.0
2.1
piperacillin
at 25°C in 100 mM sodium phosphate (pH 7.0)
2.11
piperacillin
-
wild-type enzyme
2.2
piperacillin
30°C, pH not specified in the publication
2.2
piperacillin
pH 7.5, 25°C, recombinant His-tagged mutant E164A
2.46
piperacillin
pH 7.5, 25°C, recombinant His-tagged mutant E162A
2.7
piperacillin
TEM-149 wild-type, pH 7.0, 25°C
3
piperacillin
30°C, pH 7.0
3.3
piperacillin
pH 7.2, 25°C
4
piperacillin
at 30°C in 100 mM sodium phosphate buffer, pH 7.0
4.31
piperacillin
-
mutant enzyme K232N
5
piperacillin
30°C, pH 7.0
5
piperacillin
-
in 0.01 M phosphate buffer (pH 7.0), at 25°C
5
piperacillin
TEM-149 mutant T182M, pH 7.0, 25°C
5.7
piperacillin
-
mutant enzyme D170N
6.9
piperacillin
25°C, pH 7.0
8
piperacillin
pH and temperature not specified in the publication
9
piperacillin
pH not specified in the publication, 30°C, recombinant enzyme
11
piperacillin
pH 7.0, 30°C
11.5
piperacillin
-
in 100 mM MES (pH 6.4)
14.61
piperacillin
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
22.02
piperacillin
pH 8.0, 30°C, recombinant wild-type enzyme
38
piperacillin
at 30°C in 100 mM sodium phosphate (pH 7)
39
piperacillin
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
42
piperacillin
plasmid pBC
-
pH 7.4, 22°C, T237A mutant enzyme
42
piperacillin
plasmid pBC
-
pH 7.4, 22°C, T237S mutant enzyme
44.7
piperacillin
-
at 25°C in 50 mM sodium phosphate (pH 7.0)
47
piperacillin
-
enzyme 1, BRO-1
51
piperacillin
-
enzyme 2, BRO-2
51
piperacillin
plasmid pBC
-
pH 7.4, 22°C, wild-type enzyme
100
piperacillin
-
30°C, pH 7.5
280
piperacillin
pH 7.5, 37°C
328
piperacillin
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
677
piperacillin
pH 7.5, 25°C, recombinant His-tagged wild-type enzyme
898
piperacillin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
976
piperacillin
-
pH 7.4, 22°C, wild-type enzyme SHV-1
3.3
Sulbactam
in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4
20
Sulbactam
-
in 50 mM phosphate buffer (pH 7.0), at 30°C
8.2
tazobactam
-
8.2
tazobactam
enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
0.1
ticarcillin
in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C
0.3
ticarcillin
30°C, pH 7.0
1
ticarcillin
30°C, pH 7.0
1.7
ticarcillin
30°C, pH not specified in the publication
1.722
ticarcillin
pH 8.0, 30°C, recombinant wild-type enzyme
2.3
ticarcillin
25°C, pH 7.0
5
ticarcillin
at 30°C in 100 mM sodium phosphate (pH 7)
5
ticarcillin
pH and temperature not specified in the publication
6
ticarcillin
30°C, pH 7.0
8.2
ticarcillin
beta-lactamases CTX-M-25
15
ticarcillin
30°C, pH 7.0
30
ticarcillin
pH 7.5, 30°C
84
ticarcillin
beta-lactamases CTX-M-26
190
ticarcillin
pH not specified in the publication, 30°C, recombinant enzyme
220
ticarcillin
pH 7.5, 37°C
additional information
aztreonam
unable to determine as Km is too high
additional information
aztreonam
-
unable to determine as Km is too high
additional information
cefepime
above 8, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
additional information
cefepime
-
above 8, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
additional information
ertapenem
above 25, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
additional information
ertapenem
-
above 25, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin
additional information
additional information
no hydrolysis detected with enzyme concentrations up to 4 microM for temocillin, cefepime, imipenem, and aztreonam
-
additional information
additional information
-
no hydrolysis detected with enzyme concentrations up to 4 microM for temocillin, cefepime, imipenem, and aztreonam
-