3.5.2.5: allantoinase

This is an abbreviated version!
For detailed information about allantoinase, go to the full flat file.

Word Map on EC 3.5.2.5

3.5.2.5

allantoic

ureide

uricase

allantoicase

urate

hydantoinase

dihydropyrimidinase

ureidoglycolate

hydantoin

imidase

dihydroorotase

amidohydrolases

1.7.3.3

diagnostics

Reaction

Synonyms

AaALN, allantoin amidohydrolase, allantoinase, ALLase, AllBali, ALN, aln-1, aln-2, Fe-allantoinase, IsoI, IsoII, metal-dependent allantoinase, metal-independent allantoinase, Os04g0680400, OsALN, puuE, Salk_142607, Salk_146783, zinc-amended allantoinase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.2 In cyclic amides

3.5.2.5 allantoinase

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Results	in table
7	Activating Compound
5472	AA Sequence
1	Application
1	CAS Registry Number
16	Cloned(Commentary)
3	Crystallization (Commentary)
6	Expression
18	General Information
3	IC50 Value
40	Inhibitors
4	kcat/KM [mM/s]
2	Ki Value [mM]
36	KM Value [mM]
15	Localization
10	Metals/Ions
20	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
67	Organism
7	Pathway
16	PDB ID
20	pH Optimum
2	pH Range
4	Posttranslational Modification
32	Protein Variants
29	Purification (Commentary)
2	Reaction
3	Reaction Type
58	Reference
1	Renatured (Commentary)
58	Source Tissue
24	Specific Activity [micromol/min/mg]
6	Storage Stability
74	Substrates and Products (Substrate)
15	Subunits
45	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 3.5.2.5 - allantoinase

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15 entries

DEAE-Sephacel column chromatography, MonoQ HR column chromatography, and Sephacryl S-300 gel filtration

Phaseolus vulgaris

689405

native enzyme from seeds

Glycine max

684413

native isozymes 1 and 2 from developing fruits 150fold and 286fold, respectively, to homogeneity involving heat treatment at 55°C for 20 min, anion exchange chromatography, gel filtration, and another step of anion exchange chromatography

Phaseolus vulgaris

689405

recombinant by gel filtration and affinity chromatography to near homogeneity

Ctenocephalides felis

668941

recombinant enzyme

2 entries

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis

Salmonella enterica

733422

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, ultrafiltration, and dialysis

Bacillus licheniformis

Q65LN0

733032

recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, gel fltration, and ultrafiltration

Bacillus licheniformis

L0CR34

733438

Sephacryl S-200 column gel filtration chromatography at 0-4°C

Lithobates catesbeianus

650627