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3.5.2.5: allantoinase

This is an abbreviated version!
For detailed information about allantoinase, go to the full flat file.

Word Map on EC 3.5.2.5

Reaction

(S)-allantoin
+
H2O
=
Allantoate

Synonyms

AaALN, allantoin amidohydrolase, allantoinase, ALLase, AllBali, ALN, aln-1, aln-2, Fe-allantoinase, IsoI, IsoII, metal-dependent allantoinase, metal-independent allantoinase, Os04g0680400, OsALN, puuE, Salk_142607, Salk_146783, zinc-amended allantoinase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.5 allantoinase

Crystallization

Crystallization on EC 3.5.2.5 - allantoinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml 14 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 20% w/v PEG 3350, and 0.1 M potassium thiocyanate, pH 6.5, equilibration against 0.5 ml reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution