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3.5.2.3: dihydroorotase

This is an abbreviated version!
For detailed information about dihydroorotase, go to the full flat file.

Word Map on EC 3.5.2.3

Reaction

(S)-dihydroorotate
+
H2O
=
N-carbamoyl-L-aspartate

Synonyms

amidohydrolase family protein, BcDHOase, CAD, carbamoylaspartic dehydrase, Class I DHOase, DHO, DHOase, dihydroorotase, dihydroorotase domain, dihydroorotate dehydrolase, hDHOase, huDHOase, human DHOase domain, LdDHOase, More, pyrC, type I DHOase, type II DHO, VcDHO, YpDHO

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.3 dihydroorotase

Specific Activity

Specific Activity on EC 3.5.2.3 - dihydroorotase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0014
-
crude extract
0.4985
-
after 356fold purification
0.68
purified recombinant detagged enzyme, pH 8.3, 25°C
10.57
presence of Mn2+ in culture medium, pH 9.0, 25°C
14.46
presence of Ni2+ in culture medium, pH 9.0, 25°C
18
-
L-dihydroorotate as substrate
18.3
in the biosynthetic direction
18.33
purified recombinant enzyme
18.4
in the degradative direction
2.66
-
-
22.22
presence of Mg2+ in culture medium, pH 9.0, 25°C
22.79
presence of Co2+ in culture medium, pH 9.0, 25°C
68
-
L-carbamoylaspartate as substrate
7.14
no addition of metal ions to culture medium, pH 9.0, 25°C