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3.5.2.3: dihydroorotase

This is an abbreviated version!
For detailed information about dihydroorotase, go to the full flat file.

Word Map on EC 3.5.2.3

Reaction

(S)-dihydroorotate
+
H2O
=
N-carbamoyl-L-aspartate

Synonyms

amidohydrolase family protein, BcDHOase, CAD, carbamoylaspartic dehydrase, Class I DHOase, DHO, DHOase, dihydroorotase, dihydroorotase domain, dihydroorotate dehydrolase, hDHOase, huDHOase, human DHOase domain, LdDHOase, More, pyrC, type I DHOase, type II DHO, VcDHO, YpDHO

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.3 dihydroorotase

Cloned

Cloned on EC 3.5.2.3 - dihydroorotase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into expression vector pET21-DEST, expression in Escherichia coli
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli SO1263 cells
-
expressed in Escherichia coli strain SO1263
expressed in Escherichia coli strain X7014
expression in Escherichia coli
expression in Escherichia coli mutant strain SØ1263 and strain X7014
expression in Escherichia coli strain BL21
expression in Escherichia coli strain SØ1263/pyrC-
-
expression in Escherichia coli strain X7014a
expression in Escherichia coli, His-tag
-
expression in Escherichia colipyrC mutant strain X7014a, genes pyrC(PA3527) and pyrC2(PA5541) can complement DHOase mutant, pyrC constitutively expressed, pyrC2 can only complement the DHOase mutant when expressed from the lacZalpha promotor
expression of yeast-hamster chimeric proteins in Escherichia coli
gene DHO, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, quantitative real-time PCR expression analysis
gene pyrC, DNA and amino acid sequence determination and analysis, sequence comparison, expression of N-terminal His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3) with an HRV 3C protease recognition site inserted between the SUMO tag and SaPyrC to allow for improved cleavage by HRV protease, method optimization
gene pyrC, recombinant enzyme expression in Escherichia coli strain Rosetta-gami 2(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene pyrC, recombinant expression of His6-tagged wildtype and mutant enzymes in Escherichia coli strain BL21(DE3) Gold
gene pyrC, recombinant expression of wild-type and mutant DHO enzymes in Escherichia coli strain BL21(DE3), coexpression with pyrB encoding with aspartate transcarbamoylase (ATC, EC 2.1.3.2)
gene pyrC, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
human cad promoter cloned into pGL3 basic vector
-
overexpression in Escherichia coli BL21(DES/pLysS)
-
overexpression in Escherichia coli strain BL21(DE3)
-
overexpression in Escherichia coli strain SØ1263/pyrC-
-
overproduction in transformed hamster cell line 165-23
Betapolyomavirus macacae
-
recombinant expression of the mutant His-tagged enzyme
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
with a fusion peptide containing six histidine residues at the amino terminus