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3.5.2.16: maleimide hydrolase

This is an abbreviated version!
For detailed information about maleimide hydrolase, go to the full flat file.

Word Map on EC 3.5.2.16

Reaction

Maleimide
+
H2O
=
maleamic acid

Synonyms

CIH, cyclic imide hydrolase, cyclic-imide amidohydrolase (decyclicizing), imidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.16 maleimide hydrolase

Engineering

Engineering on EC 3.5.2.16 - maleimide hydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C108G
72% of wild-type activity. Like wild-type, mutant forms tetramers. It has a high binding ability for Zn2+
C7G
complete loss of activity. Mutant is a mixture of monomers and oligomers and displays decreased binding of Zn2+
C7G/C108G
H151G
-
120% of wild-type activity
H247A
-
decrease in absorbance at 500 nm by 32.4%. Less than 10% of wild-type activity
H86A
-
decrease in absorbance at 500 nm by 14.7%. Less than 10% of wild-type activity
C108G
-
72% of wild-type activity. Like wild-type, mutant forms tetramers. It has a high binding ability for Zn2+
-
C7G
-
complete loss of activity. Mutant is a mixture of monomers and oligomers and displays decreased binding of Zn2+
-
C7G/C108G
H151G
-
120% of wild-type activity
-
H247A
-
decrease in absorbance at 500 nm by 32.4%. Less than 10% of wild-type activity
-
H86A
-
decrease in absorbance at 500 nm by 14.7%. Less than 10% of wild-type activity
-