3.5.2.10: creatininase

This is an abbreviated version!
For detailed information about creatininase, go to the full flat file.

Reaction

Synonyms

creatinine amidohydrolase, creatinine hydrolase, CrnA

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.2 In cyclic amides

                3.5.2.10 creatininase

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Results	in table
1	Activating Compound
2108	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
6	Crystallization (Commentary)
5	Disease/ Diagnostics
1	General Stability
19	Inhibitors
8	kcat/KM [mM/s]
13	KM Value [mM]
13	Metals/Ions
10	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
18	Organism
1	Oxidation Stability
5	Pathway
72	PDB ID
5	pH Optimum
4	pH Range
3	pH Stability
1	Posttranslational Modification
9	Protein Variants
5	Purification (Commentary)
4	Reaction
1	Reaction Type
17	Reference
1	Renatured (Commentary)
2	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
19	Substrates and Products (Substrate)
8	Subunits
3	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
4	Temperature Stability [°C]
8	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.5.2.10 - creatininase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.13

Pseudomonas putida

P83772

mutant E122Q, presence of Zn2+, pH not specified in the publication, temperature not specified in the publication

712749

100

Pseudomonas sp.

-

-

172082

13.9

Pseudomonas putida

P83772

mutant E122Q, presence of Mn2+, pH not specified in the publication, temperature not specified in the publication

712749

140

Pseudomonas putida

P83772

mutant Y121A, presence of Mn2+, pH not specified in the publication, temperature not specified in the publication

712749

1560

Pseudomonas putida

P83772

wild-type, presence of Mn2+, pH not specified in the publication, temperature not specified in the publication

712749

178

Pseudomonas putida

P83772

wild-type, presence of EDTA, semi-apo-enzyme, pH not specified in the publication, temperature not specified in the publication

712749

370

Pseudomonas putida

P83772

wild-type, presence of Zn2+, pH not specified in the publication, temperature not specified in the publication

712749

4.9

Pseudomonas putida

P83772

mutant W154F, presence of Mn2+, pH not specified in the publication, temperature not specified in the publication

712749

488

Pseudomonas putida

-

-

172079

740

Pseudomonas putida

P83772

mutant W174F, presence of Mn2+, pH not specified in the publication, temperature not specified in the publication

712749

additional information

Pseudomonas putida

-

by Escherichia coli transformants pCRN741 without and with IPTG 0.678 U per ml

172078